Abstract: A holding device for a dried biological fluid spotting membrane includes a plastic top face, a plastic bottom face, and a pocket disposed between the top face and the bottom face. The plastic top face includes a window formed therein. The pocket is configured for receiving a dried biological fluid spotting membrane that is accessible through the window by a user during dried biological fluid spot testing. A method for assembling a holding device includes positioning the membrane within a pocket of a plastic membrane housing, and securing the membrane within the pocket. The holding device may be utilized for forming dried biological fluid spots and extracting samples therefrom for analysis.