Patents by Inventor Ivo Piest

Ivo Piest has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • siRNA knockout assay method and constructs
    Publication number: 20050214851
    Abstract: Isolated polynucleotides, and vectors including the same, are disclosed as useful for down-regulation of specific RNA in cells, including a first sequence of about 17 to about 23 nucleotides, complementary to said RNA, and linked to a second sequence capable of forming a loop when said second sequence is RNA. The polynucleotides include self-complementing single-stranded polynucleotides, including a third sequence linked by said second sequence where all nucleotides in said first and said third sequences are complementary. Functional genomic, diagnostic and therapeutic methods are disclosed that involve reducing the amount of a unique RNA sequence in cells using a vector encoding the self-complementing polynucleotide including a first sequence complementary to said RNA sequence. Methods are also disclosed for preparing the polynucleotides, vectors, libraries of vectors, and the temporary knock-down of proteins, such as lethal proteins, during virus or recombinant protein production.
    Type: Application
    Filed: May 13, 2005
    Publication date: September 29, 2005
    Applicant: Galapagos N.V.
    Inventors: Gert-Jan Arts, Ellen Langemeijer, Ivo Piest, Helmuth Van Es, Godefridus Michiels
  • siRNA knockout assay method and constructs
    Publication number: 20030198627
    Abstract: Isolated polynucleotides, and vectors including the same, are disclosed as useful for down-regulation of specific RNA in cells, including a first sequence of about 17 to about 23 nucleotides, complementary to said RNA, and linked to a second sequence capable of forming a loop when said second sequence is RNA. The polynucleotides include self-complementing single-stranded polynucleotides, including a third sequence linked by said second sequence where all nucleotides in said first and said third sequences are complementary. Functional genomic, diagnostic and therapeutic methods are disclosed that involve reducing the amount of a unique RNA sequence in cells using a vector encoding the self-complementing polynucleotide including a first sequence complementary to said RNA sequence. Methods are also disclosed for preparing the polynucleotides, vectors, libraries of vectors, and the temporary knock-down of proteins, such as lethal proteins, during virus or recombinant protein production.
    Type: Application
    Filed: August 23, 2002
    Publication date: October 23, 2003
    Inventors: Gert-Jan Arts, Ellen Vera Langemeijer, Ivo Piest, Helmuth Hendrikus Gerardus Van Es, Godefridus Augustinus Maria Michiels
  • Swap/counter selection: a rapid cloning method
    Publication number: 20020177229
    Abstract: A method for transferring a polynucleic acid sequence from a donor vector to an acceptor vector wherein said donor vector includes a first antibiotic resistance functioning sequence (ARFS) and said acceptor vector includes a second ARFS comprising:
    Type: Application
    Filed: January 18, 2002
    Publication date: November 28, 2002
    Inventors: Ivo Piest, Godefridus A.M. Michiels, Helmuth H.G. van Es, Onno van de Stolpe