Patents by Inventor Ivor T. Knight
Ivor T. Knight has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 11351545Abstract: A method and system have been provided to perform high speed nucleic acid melting analysis while still obtaining accurate melting curve sufficient for genotyping. This rapid ability to interrogate DNA should be useful whenever time to result is important, such as in molecular point of care testing. Specifically, microfluidics enables genotyping by melting analysis at rates up to 50° C./s, requiring less than is to acquire an entire melting curve. High speed melting reduces the time for melting analysis, decreases errors, and improves genotype discrimination of small amplicons.Type: GrantFiled: July 31, 2017Date of Patent: June 7, 2022Assignees: Canon U.S.A., Inc., University of Utah Research FoundationInventors: Robert J. Pryor, Carl T. Wittwer, Scott O. Sundberg, Ivor T. Knight, Joseph T. Myrick, Robert A. Palais, Jeanette Y. Paek
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Patent number: 11162910Abstract: The present invention provides novel methods and devices that employ microfluidic technology to generate molecular melt curves. In particular, the devices and methods in accordance with the invention are useful in providing for the analysis of PCR amplification products.Type: GrantFiled: June 27, 2016Date of Patent: November 2, 2021Assignees: CALIPER LIFE SCIENCES, INC., Canon U.S.A., Inc.Inventors: Steven A. Sundberg, Michael R. Knapp, Ivor T. Knight, Deborah J. Boles, Aaron Rulison, Wesley B. Dong, Andrew Fabans, Edward Donlon, Robert Moti, Michael Slater
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Patent number: 10871460Abstract: The present invention provides novel methods and devices that employ microfluidic technology to generate molecular melt curves. In particular, the devices and methods in accordance with the invention are useful in providing for the analysis of PCR amplification products.Type: GrantFiled: May 25, 2018Date of Patent: December 22, 2020Assignee: Canon U.S.A., Inc.Inventors: Ivor T. Knight, Deborah John Boles, Aaron Rulison, Wesley B. Dong, Andrew Fabans, Allen Boronkay, Edward Donlon, Robert Moti, Michael Slater, Steven A. Sundberg, Michael R. Knapp
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Patent number: 10814321Abstract: At least one exemplary embodiment of the invention is directed to a molecular diagnostic device that comprises a cartridge configured to eject samples comprising genomic material into a microfluidic chip that comprises an amplification area, a detection area, and a matrix analysis area.Type: GrantFiled: June 4, 2018Date of Patent: October 27, 2020Assignee: Canon U.S.A., Inc.Inventors: Hiroshi Inoue, Ivor T. Knight, Gregory A. Dale, Rita R. Colwell
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Patent number: 10363558Abstract: The present invention relates to systems and methods for the real time processing of nucleic acid during polymerase chain reaction (PCR) and thermal melt applications. According to an aspect of the invention, a system for the rapid serial processing of multiple nucleic acid assays is provided. In one embodiment, the system includes, but is not limited to: a microfluidic cartridge having microfluidic (flow-through) channels, a fluorescence imaging system, a temperature measurement and control system; a pressure measurement and control system for applying variable pneumatic pressures to the microfluidic cartridge; a storage device for holding multiple reagents (e.g., a well-plate); a liquid handling system comprising at least one robotic pipettor for aspirating, mixing, and dispensing reagent mixtures to the microfluidic cartridge; systems for data storage, processing, and output; and a system controller to coordinate the various devices and functions.Type: GrantFiled: August 24, 2015Date of Patent: July 30, 2019Assignee: Canon U.S. Life Sciences, Inc.Inventors: Ivor T. Knight, Kenton C. Hasson, Johnathan S. Coursey, Hongye Liang, Sami Kanderian, Gregory H. Owen, Weidong Cao, Ying-Xin Wang, Scott Corey, Ben Lane, Conrad Laskowski, Alex Flamm, Brian Murphy, Eric Schneider, Takayoshi Hanagata, Hiroshi Inoue, Shulin Zeng, Brian Bean, Franklin Regan
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Publication number: 20180321170Abstract: The present invention provides novel methods and devices that employ microfluidic technology to generate molecular melt curves. In particular, the devices and methods in accordance with the invention are useful in providing for the analysis of PCR amplification products.Type: ApplicationFiled: May 25, 2018Publication date: November 8, 2018Applicants: Canon U.S. Life Sciences, Inc., CALIPER LIFE SCIENCES, INC.Inventors: Ivor T. Knight, Deborah John Boles, Aaron Rulison, Wesley B. Dong, Andrew Fabans, Allen Boronkay, Edward Donlon, Robert Moti, Michael Slater, Steven A. Sundberg, Michael R. Knapp
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Publication number: 20180311661Abstract: At least one exemplary embodiment of the invention is directed to a molecular diagnostic device that comprises a cartridge configured to eject samples comprising genomic material into a microfluidic chip that comprises an amplification area, a detection area, and a matrix analysis area.Type: ApplicationFiled: June 4, 2018Publication date: November 1, 2018Applicant: Canon U.S. Life Sciences, Inc.Inventors: Hiroshi Inoue, Ivor T. Knight, Gregory A. Dale, Rita R. Colwell
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Patent number: 9987627Abstract: At least one exemplary embodiment of the invention is directed to a molecular diagnostic device that comprises a cartridge configured to eject samples comprising genomic material into a microfluidic chip that comprises an amplification area, a detection area, and a matrix analysis area.Type: GrantFiled: March 28, 2011Date of Patent: June 5, 2018Assignee: Canon U.S. Life Sciences, Inc.Inventors: Hiroshi Inoue, Ivor T. Knight, Gregory A. Dale, Rita R. Colwell
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Patent number: 9983155Abstract: The present invention provides novel methods and devices that employ microfluidic technology to generate molecular melt curves. In particular, the devices and methods in accordance with the invention are useful in providing for the analysis of PCR amplification products.Type: GrantFiled: March 15, 2013Date of Patent: May 29, 2018Assignees: Canon U.S. Life Sciences, Inc., CALIPER LIFE SCIENCES, INC.Inventors: Ivor T. Knight, Deborah John Boles, Aaron Rulison, Wesley B. Dong, Andrew Fabans, Allen Boronkay, Edward Donlon, Robert Moti, Michael Slater, Steven A. Sundberg, Michael R. Knapp
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Patent number: 9962692Abstract: In one aspect, the present invention provides methods, devices, and systems for ensuring that multiple components of a mixture are fully mixed in a continuous flow microfluidic system while ensuring that mixing between segments flowing through the chip is minimized. In some embodiments, the present invention includes mixing fluids in a droplet maintained at the tip of a pipette before the mixture is introduced to the microfluidic device. In another aspect, the present invention provides a pipette tip having a ratio of an outside diameter to an inside diameter that provides sufficient surface area for a droplet comprising up to the entire volume of the liquid to suspend from the pipette tip intact. In yet another aspect, the present invention provides methods, devices, and systems for delivering a reaction mixture to a microfluidic chip comprising a docking receptacle, an access tube and a reservoir.Type: GrantFiled: August 31, 2011Date of Patent: May 8, 2018Assignee: Canon U.S. Life Sciences, Inc.Inventors: Ivor T. Knight, Scott Corey, Ben Lane, Conrad Laskowski, Alex Flamm, Brian Murphy
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Publication number: 20180111125Abstract: A method and system have been provided to perform high speed nucleic acid melting analysis while still obtaining accurate melting curve sufficient for genotyping. This rapid ability to interrogate DNA should be useful whenever time to result is important, such as in molecular point of care testing. Specifically, microfluidics enables genotyping by melting analysis at rates up to 50° C./s, requiring less than is to acquire an entire melting curve. High speed melting reduces the time for melting analysis, decreases errors, and improves genotype discrimination of small amplicons.Type: ApplicationFiled: July 31, 2017Publication date: April 26, 2018Applicant: Canon U.S. Life Sciences, Inc.Inventors: Robert J. Pryor, Carl T. Wittwer, Scott O. Sundberg, Ivor T. Knight, Joseph T. Myrick, Robert A. Palais, Jeanette Y. Paek
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Patent number: 9580744Abstract: The present invention provides a method for performing thermal melt analysis using a microfluidic device, the method comprising providing a microfluidic device having at least one microfluidic channel, introducing fluid comprising into the at least one microfluidic channel, continuously flowing the fluid through the at least one microfluidic channel while varying the temperature of the entire fluid stream as it moves through the at least one microfluidic channel by uniformly heating the entire fluid stream, and measuring, while continuously flowing the fluid through the at least one microfluidic channel, a detectable property emanating from the fluid.Type: GrantFiled: July 15, 2014Date of Patent: February 28, 2017Assignee: Canon U.S. Life Sciences, Inc.Inventors: Ivor T. Knight, Deborah J. Boles
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Publication number: 20160377562Abstract: The present invention provides novel methods and devices that employ microfluidic technology to generate molecular melt curves. In particular, the devices and methods in accordance with the invention are useful in providing for the analysis of PCR amplification products.Type: ApplicationFiled: June 27, 2016Publication date: December 29, 2016Applicants: CALIPER LIFE SCIENCES, INC., CANON U.S. LIFE SCIENCES, INC.Inventors: Steven A. Sundberg, Michael R. Knapp, Ivor T. Knight, Deborah J. Boles, Aaron Rulison, Wesley B. Dong, Andrew Fabans, Allen Boronkay, Edwawrd Donlon, Robert Moti, Michael Slater
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Publication number: 20160325280Abstract: An assembly for performing micro-fluidic assays includes a micro-fluidic chip with access ports and micro-channels in communication with the access ports and a fluid cartridge having internal, fluid-containable chambers and a nozzle associated with each internal chamber that is configured to be coupled with an access port. Reaction fluids, such as sample material, buffer, and/or reagent, contained within the cartridge are dispensed from the cartridge into the access ports and micro-channels of the micro-fluidic chip. Embodiments of the invention include a cartridge which includes a waste compartment for receiving used DNA and other reaction fluids from the micro-channel at the conclusion of the assay.Type: ApplicationFiled: March 7, 2016Publication date: November 10, 2016Applicant: Canon U.S. Life Sciences, Inc.Inventors: Gregory A. DALE, Ivor T. KNIGHT
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Patent number: 9376718Abstract: The present invention provides novel methods and devices that employ microfluidic technology to generate molecular melt curves. In particular, the devices and methods in accordance with the invention are useful in providing for the analysis of PCR amplification products.Type: GrantFiled: April 11, 2012Date of Patent: June 28, 2016Assignees: Caliper Life Sciences, Inc., Canon U.S. Life Sciences, Inc.Inventors: Steven A. Sundberg, Michael R. Knapp, Ivor T. Knight, Deborah J. Boles, Aaron J. Rulison, Wesley B. Dong, Edward P. Donlon, Robert J. Moti, Andrew G. Fabans, Allen R. Boronkay, Michael Slater
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Patent number: 9278321Abstract: An assembly for performing micro-fluidic assays includes a micro-fluidic chip with access ports and micro-channels in communication with the access ports and a fluid cartridge having internal, fluid-containable chambers and a nozzle associated with each internal chamber that is configured to be coupled with an access port. Reaction fluids, such as sample material, buffer, and/or reagent, contained within the cartridge are dispensed from the cartridge into the access ports and micro-channels of the micro-fluidic chip. Embodiments of the invention include a cartridge which includes a waste compartment for receiving used DNA and other reaction fluids from the micro-channel at the conclusion of the assay.Type: GrantFiled: September 5, 2007Date of Patent: March 8, 2016Assignee: Canon U.S. Life Sciences, Inc.Inventors: Gregory A. Dale, Ivor T. Knight
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Publication number: 20160051985Abstract: The present invention relates to systems and methods for the real time processing of nucleic acid during polymerase chain reaction (PCR) and thermal melt applications. According to an aspect of the invention, a system for the rapid serial processing of multiple nucleic acid assays is provided. In one embodiment, the system includes, but is not limited to: a microfluidic cartridge having microfluidic (flow-through) channels, a fluorescence imaging system, a temperature measurement and control system; a pressure measurement and control system for applying variable pneumatic pressures to the microfluidic cartridge; a storage device for holding multiple reagents (e.g., a well-plate); a liquid handling system comprising at least one robotic pipettor for aspirating, mixing, and dispensing reagent mixtures to the microfluidic cartridge; systems for data storage, processing, and output; and a system controller to coordinate the various devices and functions.Type: ApplicationFiled: August 24, 2015Publication date: February 25, 2016Applicant: Canon U.S. Life Sciences, Inc.Inventors: Ivor T. KNIGHT, Kenton C. Hasson, Johnathan S. Coursey, Hongye Liang, Sami Kanderian, Gregory H. Owen, Weidong Cao, Ying-Xin Wang, Scott Corey, Ben Lane, Conrad Laskowski, Alex Flamm, Brian Murphy, Eric Schneider, Takayoshi Hanagata, Hiroshi Inoue, Shulin Zeng, Brian Bean, Franklin Regan
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Patent number: 9114399Abstract: The present invention relates to systems and methods for the real time processing of nucleic acid during polymerase chain reaction (PCR) and thermal melt applications. According to an aspect of the invention, a system for the rapid serial processing of multiple nucleic acid assays is provided. In one embodiment, the system includes, but is not limited to: a microfluidic cartridge having microfluidic (flow-through) channels, a fluorescence imaging system, a temperature measurement and control system; a pressure measurement and control system for applying variable pneumatic pressures to the microfluidic cartridge; a storage device for holding multiple reagents (e.g., a well-plate); a liquid handling system comprising at least one robotic pipettor for aspirating, mixing, and dispensing reagent mixtures to the microfluidic cartridge; systems for data storage, processing, and output; and a system controller to coordinate the various devices and functions.Type: GrantFiled: August 31, 2011Date of Patent: August 25, 2015Assignee: Canon U.S. Life Sciences, Inc.Inventors: Ivor T. Knight, Kenton C. Hasson, Johnathan S. Coursey, Hongye Liang, Sami Kanderian, Gregory H. Owen, Weidong Cao, Ying-Xin Wang, Scott Corey, Ben Lane, Conrad Laskowski, Alex Flamm, Brian Murphy, Eric Schneider, Takayoshi Hanagata, Hiroshi Inoue, Shulin Zeng, Brian Bean, Franklin Regan
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Patent number: 9114398Abstract: A method and device for digital multiplex PCR assays employ a microfluidic chip for performing real-time, continuous flow PCR within microchannels of the chip. A stream of sample material is introduced into each microchannel and alternating boluses of assay-specific reagents and buffer are introduced into the stream to form sequentially configured test boluses. A PCR procedure is performed on the test boluses followed by a thermal melt procedure. During the thermal melt procedure, fluorescent output is detected and fluorescence vs temperature data is collected and compared to expected normal correlations. The results, positive or negative, are converted to digital format, with positive results designated as “1” and negative results designated as “0”, or vice versa.Type: GrantFiled: November 29, 2007Date of Patent: August 25, 2015Assignee: Canon U.S. Life Sciences, Inc.Inventors: Ivor T. Knight, Hiroshi Inoue
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Patent number: D767782Type: GrantFiled: November 13, 2014Date of Patent: September 27, 2016Assignee: Canon U.S. Life Sciences, Inc.Inventors: Weidong Cao, Kenton C. Hasson, Ivor T. Knight, Takayoshi Hanagata, Chris Felice, Kazuhiko Hasegawa, Ronald Kurz, Scott Sundberg, Ralph McCann, Jason Clemons