Patents by Inventor Iwao Kiyokawa

Iwao Kiyokawa has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • DNA encoding canine monomeric CRP and expression vector containing the DNA
    Patent number: 9359416
    Abstract: Pentameric CRP is produced at a high efficiency by transferring DNA, which encodes monomeric CRP, into a silkworm to thereby construct a transgenic silkworm and then collecting and purifying pentameric CRP that is produced by the transgenic silkworm constructed above.
    Type: Grant
    Filed: September 16, 2014
    Date of Patent: June 7, 2016
    Assignees: NITTO BOSEKI CO., LTD., NATIONAL INSTITUTE OF AGROBIOLOGICAL SCIENCES
    Inventors: Iwao Kiyokawa, Yuji Arimatsu, Toshihide Miura, Ryo Kojima, Hideki Sezutsu, Keiro Uchino, Isao Kobayashi, Toshiki Tamura
  • 5.9 kDa peptide immunoassay method
    Patent number: 9017959
    Abstract: Disclosed is an immunoassay method whereby a 5.9 kDa peptide which results from the degradation of the ?-E chain and ? chain of human fibrinogens and which is used as a peptide marker for diagnosing hepatic disease can be specifically assayed in a biological sample containing contaminating peptides by bringing antibodies that recognize the N terminal of said peptide marker and antibodies that recognize the C terminal of said peptide marker into contact with said peptide marker, forming immune complexes of said peptide marker and the two antibodies, and immunoassaying the obtained immune complexes.
    Type: Grant
    Filed: October 13, 2010
    Date of Patent: April 28, 2015
    Assignee: Nitto Boseki Co., Ltd.
    Inventors: Wataru Kikuchi, Momoe Sato, Kenta Noda, Iwao Kiyokawa, Toshihide Miura, Ryo Kojima, Fumio Nomura, Kazuyuki Sogawa
  • Antibody-producing transgenic silkworms and methods for producing the same
    Patent number: 8952215
    Abstract: The present inventors produced transgenic silkworms which comprise a promoter of a DNA encoding a protein specifically expressed in the silk gland and a DNA encoding a recombinant antibody whose expression is regulated directly or indirectly by the promoter, and which secrete the recombinant antibody into the silk gland. The recombinant antibodies produced from the silk gland of the transgenic silkworms were confirmed to be active.
    Type: Grant
    Filed: October 18, 2006
    Date of Patent: February 10, 2015
    Assignees: Nitto Boseki Co., Ltd., National Institute of Agrobiological Sciences
    Inventors: Toshiki Tamura, Isao Kobayashi, Toshio Kanda, Keiro Uchino, Katsuhiro Katayama, Tatsuya Ohashi, Iwao Kiyokawa, Hisae Arai, Noriyuki Funahashi
  • DNA ENCODING CANINE MONOMERIC CRP AND EXPRESSION VECTOR CONTAINING THE DNA
    Publication number: 20150037880
    Abstract: Pentameric CRP is produced at a high efficiency by transferring DNA, which encodes monomeric CRP, into a silkworm to thereby construct a transgenic silkworm and then collecting and purifying pentameric CRP that is produced by the transgenic silkworm constructed above.
    Type: Application
    Filed: September 16, 2014
    Publication date: February 5, 2015
    Inventors: Iwao KIYOKAWA, Yuji Arimatsu, Toshihide Miura, Ryo Kojima, Hideki Sezutsu, Keiro Uchino, Isao Kobayashi, Toshiki Tamura
  • Pentameric CRP-producing transgenic silkworm
    Patent number: 8865966
    Abstract: Pentameric CRP is produced at a high efficiency by transferring DNA, which encodes monomeric CRP, into a silkworm to thereby construct a transgenic silkworm and then collecting and purifying pentameric CRP that is produced by the transgenic silkworm constructed above.
    Type: Grant
    Filed: May 30, 2011
    Date of Patent: October 21, 2014
    Assignees: Nitto Boseki Co., Ltd., National Institute of Agrobiological Sciences
    Inventors: Iwao Kiyokawa, Yuji Arimatsu, Toshihide Miura, Ryo Kojima, Hideki Sezutsu, Keiro Uchino, Isao Kobayashi, Toshiki Tamura
  • METHOD FOR PRODUCING PENTAMERIC CRP, PENTAMERIC CRP-PRODUCING TRANSGENIC SILKWORM AND METHOD FOR CONSTRUCTING SAME, DNA ENCODING CANINE MONOMERIC CRP AND EXPRESSION VECTOR CONTAINING THE DNA
    Publication number: 20130212717
    Abstract: Pentameric CRP is produced at a high efficiency by transferring DNA, which encodes monomeric CRP, into a silkworm to thereby construct a transgenic silkworm and then collecting and purifying pentameric CRP that is produced by the transgenic silkworm constructed above.
    Type: Application
    Filed: May 30, 2011
    Publication date: August 15, 2013
    Applicants: NATIONAL INSTITUE OF AGROBIOLOGICAL SCIENCES, NITTO BOSEKI CO., LTD.
    Inventors: Iwao Kiyokawa, Yuji Arimatsu, Toshihide Miura, Ryo Kojima, Hideki Sezutsu, Keiro Uchino, Isao Kobayashi, Toshiki Tamura
  • Method for production of TRACP5b
    Patent number: 8426674
    Abstract: Silkworms which have (i) a DNA encoding a transcriptional regulator operably linked downstream of a promoter of a DNA encoding a protein specifically expressed in the silk gland and (ii) a DNA encoding TRACP5 operably linked downstream of a target promoter of the transcriptional regulator were produced. The result showed that active TRACP5b was produced from the silkworms. This means that TRACP5 produced from the silk gland of the silkworms undergoes processing in the silk gland that is similar to the processing taking place at bone resorption sites.
    Type: Grant
    Filed: December 28, 2007
    Date of Patent: April 23, 2013
    Assignees: Nitto Boseki Co., Ltd., National Institute of Agrobiological Sciences
    Inventors: Iwao Kiyokawa, Tatsuya Ohashi, Toshihide Miura, Katsuhiro Katayama, Toshiki Tamura, Isao Kobayashi, Hideki Sezutsu
  • MARKER FOR DETECTION AND/OR DISCRIMINATION OF NON-ALCOHOLIC STEATOHEPATITIS, METHOD FOR DETECTION AND/OR DISCRIMINATION OF NON-ALCOHOLIC STEATOHEPATITIS, AND KIT FOR USE IN THE METHOD
    Publication number: 20130089871
    Abstract: The detection of a non-alcoholic steatohepatitis patient and the accurate discrimination between a normal person and a non-alcoholic steatohepatitis patient can be achieved by measuring alanine-glyoxylate amino transferase in a sample.
    Type: Application
    Filed: June 13, 2011
    Publication date: April 11, 2013
    Applicants: NATIONAL UNIVERSITY CORPORATION CHIBA UNIVERSITY, NITTOBO MEDICAL CO., LTD., NITTO BOSEKI CO., LTD.
    Inventors: Fumio Nomura, Motoi Nishimura, Katsuhiro Katayama, Iwao Kiyokawa
  • 5.9 kDa PEPTIDE IMMUNOASSAY METHOD
    Publication number: 20120276562
    Abstract: Disclosed is an immunoassay method whereby a 5.9 kDa peptide which results from the degradation of the ?-E chain and ? chain of human fibrinogens and which is used as a peptide marker for diagnosing hepatic disease can be specifically assayed in a biological sample containing contaminating peptides by bringing antibodies that recognize the N terminal of said peptide marker and antibodies that recognize the C terminal of said peptide marker into contact with said peptide marker, forming immune complexes of said peptide marker and the two antibodies, and immunoassaying the obtained immune complexes.
    Type: Application
    Filed: October 13, 2010
    Publication date: November 1, 2012
    Applicant: Nitto Boseki Co., Ltd.
    Inventors: Wataru Kikuchi, Momoe Sato, Kenta Noda, Iwao Kiyokawa, Toshihide Miura, Ryo Kojima, Fumio Nomura, Kazuyuki Sogawa
  • METHOD FOR PRODUCTION OF TRACP5b
    Publication number: 20110239313
    Abstract: Silkworms which have (i) a DNA encoding a transcriptional regulator operably linked downstream of a promoter of a DNA encoding a protein specifically expressed in the silk gland and (ii) a DNA encoding TRACP5 operably linked downstream of a target promoter of the transcriptional regulator were produced. The result showed that active TRACP5b was produced from the silkworms. This means that TRACP5 produced from the silk gland of the silkworms undergoes processing in the silk gland that is similar to the processing taking place at bone resorption sites.
    Type: Application
    Filed: December 28, 2007
    Publication date: September 29, 2011
    Applicants: NITTO BOSEKI CO., LTD., NATIONAL INSTITUTE OF AGROBIOLOGICAL SCIENCES
    Inventors: Iwao Kiyokawa, Tatsuya Ohashi, Toshihide Miura, Katsuhiro Katayama, Toshiki Tamura, Isao Kobayashi, Hideki Sezutsu
  • TRANSGENIC SILKWORM CAPABLE OF PRODUCING ANTIBODY AND METHOD FOR PRODUCTION THEREOF
    Publication number: 20110021757
    Abstract: The present inventors produced transgenic silkworms which comprise a promoter of a DNA encoding a protein specifically expressed in the silk gland and a DNA encoding a recombinant antibody whose expression is regulated directly or indirectly by the promoter, and which secrete the recombinant antibody into the silk gland. The recombinant antibodies produced from the silk gland of the transgenic silkworms were confirmed to be active.
    Type: Application
    Filed: October 18, 2006
    Publication date: January 27, 2011
    Applicants: NATIONAL INSTITUTE OF AGROBIOLOGICAL SCIENCES, NITTO BOSEKI CO., LTD., UNITECH CO., LTD
    Inventors: Toshiki Tamura, Isao Kobayashi, Toshio Kanda, Keiro Uchino, Katsuhiro Katayama, Tatsuya Ohashi, Iwao Kiyokawa, Hisae Arai, Noriyuki Funahashi