Patents by Inventor Izumu Saito
Izumu Saito has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20210395774Abstract: The following are provided: a novel adenovirus vector having a shortened backbone; a nucleic acid vector expressing five or more Cas guide RNA; a nucleic acid vector containing a Cas protein-coding gene and a Cas guide RNA expression unit; a composition for genome editing containing these vectors; and a gene therapeutic method using these.Type: ApplicationFiled: September 24, 2019Publication date: December 23, 2021Applicant: Microbial Chemistry Research FoundationInventors: Izumu Saito, Tomoko Nakanishi
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Patent number: 8591879Abstract: The present invention provides a novel adenovirus vector for which inflammation during the in vivo administration thereof is alleviated by inhibiting the induction of expression of an adenovirus gene by a foreign promoter inserted into the adenovirus genome, and a method for producing the vector, a cell line for use in the production of the recombinant adenovirus vector, or a gene therapy method using the recombinant adenovirus vector.Type: GrantFiled: June 3, 2011Date of Patent: November 26, 2013Assignee: Dainippon Sumitomo Pharma Co., LtdInventors: Michio Nakai, Kazuo Komiya, Masashi Murata, Naoki Tohdoh, Izumu Saito
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Patent number: 8216833Abstract: A novel cosmid vector and the like effectively used in generating a recombinant adenoviral vector are provided. More specifically, there are provided a cosmid vector characterized by: (1) containing an adenoviral genome having adenoviral inverted terminal repeat sequences each having a complete nucleotide sequence, (2) having a deletion in an adenovirus E1 gene region, and (3) containing a restriction enzyme recognition sequence not present in the adenoviral genome, on both sides of the adenoviral genome; a method of generating a recombinant adenoviral vector using the cosmid vector; and a reagent for generating a recombinant adenoviral vector containing the cosmid vector as a component.Type: GrantFiled: November 19, 2003Date of Patent: July 10, 2012Assignees: Izumu Saito, Dainippon Sumitomo Pharma Co., Ltd.Inventors: Izumu Saito, Yumi Saito
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Publication number: 20120020924Abstract: The present invention provides a novel adenovirus vector for which inflammation during the in vivo administration thereof is alleviated by inhibiting the induction of expression of an adenovirus gene by a foreign promoter inserted into the adenovirus genome, and a method for producing the vector, a cell line for use in the production of the recombinant adenovirus vector, or a gene therapy method using the recombinant adenovirus vector.Type: ApplicationFiled: June 3, 2011Publication date: January 26, 2012Applicant: DAINIPPON SUMITOMO PHARMA CO., LTD.Inventors: Michio Nakai, Kazuo Komiya, Masashi Murata, Naoki Tohdoh, Izumu Saito
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Publication number: 20090104691Abstract: The present invention provides a novel adenovirus vector for which inflammation during the in vivo administration thereof is alleviated by inhibiting the induction of expression of an adenovirus gene by a foreign promoter inserted into the adenovirus genome, and a method for producing the vector, a cell line for use in the production of the recombinant adenovirus vector, or a gene therapy method using the recombinant adenovirus vector.Type: ApplicationFiled: September 16, 2008Publication date: April 23, 2009Applicant: Dainippon Sumitomo Pharma Co., Ltd.Inventors: Michio Nakai, Kazuo Komiya, Masashi Murata, Naoki Tohdoh, Izumu Saito
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Patent number: 7476539Abstract: To provide DNA comprising mutant FRT sequence which causes recombination reaction between two mutant FRT sequences having an identical sequence to each other but does not cause recombination reaction with a wild-type FRT sequence, in the presence of FLP recombinase; and a method for performing high-efficiency, gene insertion or gene replacement. A DNA comprising a mutant FRT sequence (any one of SEQ ID NOs: 2 to 5); a DNA comprising a mutant FRT sequence possessing (A) causing no specific DNA recombination reaction with wild type FRT, even if FLP recombinase is present, and (B) causing specific DNA recombination reaction with another mutant FRT sequence having an identical sequence thereto in the presence of recombinase FLP, wherein the mutant FRT sequence has substitutions of at least one nucleotide in a region other than the spacer region in the sequence and a cell which is transformed with the DNA.Type: GrantFiled: November 7, 2005Date of Patent: January 13, 2009Assignee: Dainippon Sumitomo Pharma Co., Ltd.Inventors: Izumu Saito, Yumi Saito
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Publication number: 20080319177Abstract: In accordance with the present invention, there are provided cells for expressing recombinase Cre in the presence of recombinase FLP in a FLP-dependent manner, methods of expressing recombinase Cre by introducing recombinase FLP into the above cells, methods of producing recombinant viral vectors using cells that express recombinase Cre in the presence of recombinase FLP in a FLP-dependent manner, and methods of producing recombinant adenovirus vectors using the above method of producing Cre and the above method of producing recombinant viral vectors.Type: ApplicationFiled: April 28, 2008Publication date: December 25, 2008Applicant: Dainippon Sumitomo Pharma Co., Ltd.Inventors: Izumu SAITO, Yumi Kanegae
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Patent number: 7445929Abstract: The present invention provides a novel adenovirus vector for which inflammation during the in vivo administration thereof is alleviated by inhibiting the induction of expression of an adenovirus gene by a foreign promoter inserted into the adenovirus genome, and a method for producing the vector, a cell line for use in the production of the recombinant adenovirus vector, or a gene therapy method using the recombinant adenovirus vector.Type: GrantFiled: May 24, 2001Date of Patent: November 4, 2008Assignee: Dainippon Sumitomo Pharma Co., Ltd.Inventors: Michio Nakai, Kazuo Komiya, Masashi Murata, Naoki Tohdoh, Izumu Saito
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Publication number: 20060172421Abstract: A novel cosmid vector and the like effectively used in generating a recombinant adenoviral vector are provided. More specifically, there are provided a cosmid vector characterized by: (1) containing an adenoviral genome having adenoviral inverted terminal repeat sequences each having a complete nucleotide sequence, (2) having a deletion in an adenovirus E1 gene region, and (3) containing a restriction enzyme recognition sequence not present in the adenoviral genome, on both sides of the adenoviral genome; a method of generating a recombinant adenoviral vector using the cosmid vector; and a reagent for generating a recombinant adenoviral vector containing the cosmid vector as a component.Type: ApplicationFiled: November 19, 2003Publication date: August 3, 2006Inventors: Izumu Saito, Yumi Saito
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Patent number: 7060499Abstract: To provide DNA comprising mutant FRT sequence which causes recombination reaction between two mutant FRT sequences having an identical sequence to each other but does not cause recombination reaction with a wild-type FRT sequence, in the presence of FLP recombinase; and a method for performing high-efficiency, gene insertion or gene replacement. A DNA comprising a mutant FRT sequence. A DNA comprising a mutant FRT sequence possessing (A) causing no specific DNA recombination reaction with wild type FRT, even if FLP recombinase is present, and (B) causing specific DNA recombination reaction with another mutant FRT sequence having an identical sequence thereto in the presence of recombinase FLP; gene replacement method using the DNA in the presence of recombinase FLP; and a specific DNA recombination method, characterized in that a specific DNA recombination reaction is carried out by using two mutant FRT sequences in the presence of recombinase FLP.Type: GrantFiled: September 28, 2000Date of Patent: June 13, 2006Assignees: Dainippon Sumitomo Pharma Co., Ltd.Inventors: Izumu Saito, Yumi Saito
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Publication number: 20060094111Abstract: To provide DNA comprising mutant FRT sequence which causes recombination reaction between two mutant FRT sequences having an identical sequence to each other but does not cause recombination reaction with a wild-type FRT sequence, in the presence of FLP recombinase; and a method for performing high-efficiency, gene insertion or gene replacement.Type: ApplicationFiled: November 7, 2005Publication date: May 4, 2006Inventors: Izumu Saito, Yumi Saito
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Publication number: 20060003443Abstract: In accordance with the present invention, there are provided cells for expressing recombinase Cre in the presence of recombinase FLP in a FLP-dependent manner, methods of expressing recombinase Cre by introducing recombinase FLP into the above cells, methods of producing recombinant viral vectors using cells that express recombinase Cre in the presence of recombinase FLP in a FLP-dependent manner, and methods of producing recombinant adenovirus vectors using the above method of producing Cre and the above method of producing recombinant viral vectors.Type: ApplicationFiled: September 2, 2005Publication date: January 5, 2006Inventors: Izumu Saito, Yumi Kanegae
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Patent number: 6740515Abstract: Recombinant adenoviruses comprising the following sequences in the genome: (1) a left inverted terminal repeat: (2) a packaging signal: (3) a recombinase recognition sequence located at a region in between said left inverted terminal repeat and said packaging signal: and (4) at least one more recombinase recognition sequence which is located downstream of said packaging signal and which is recognized by the recombinase that recognizes the above recombinase recognition sequence are useful as a material for constructing highly safe vectors for gene therapy in the field of gene therapy.Type: GrantFiled: August 16, 2001Date of Patent: May 25, 2004Assignee: Sumitomo Pharmaceuticals Company, LimitedInventors: Izumu Saito, Yumi Saito
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Publication number: 20040091456Abstract: The present invention provides a novel adenovirus vector for which inflammation during the in vivo administration thereof is alleviated by inhibiting the induction of expression of an adenovirus gene by a foreign promoter inserted into the adenovirus genome, and a method for producing the vector, a cell line for use in the production of said recombinant adenovirus vector, or a gene therapy method using said recombinant adenovirus vector.Type: ApplicationFiled: November 26, 2002Publication date: May 13, 2004Inventors: Michio Nakai, Kazuo Komiya, Masashi Murata, Naoki Tohdoh, Izumu Saito
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Patent number: 6465254Abstract: Highly efficient gene integration or gene replacement in the higher eucaryote including animal cells can be performed by using mutant loxP site having the following properties (a)-(c) in the present invention. (a) a nucleotide sequence wherein, in a wild-type loxP site of the following formula (SEQ ID NO: 1) derived from E.Type: GrantFiled: July 7, 2000Date of Patent: October 15, 2002Assignee: Sumitomo Pharmaceuticals Company, LimitedInventors: Izumu Saito, Keiji Tanaka
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Patent number: 6429355Abstract: A hepatitis type C animal model into which cDNA derived from hepatitis C virus has been introduced. This animal model is useful for clarification of an onset mechanism of hepatitis C and as well as for development of means for treating the disease.Type: GrantFiled: September 14, 2000Date of Patent: August 6, 2002Assignees: Tokyo Metropolitan Institute of Medical Science, Chugai Seiyaku Kabushiki KaishaInventors: Michinori Kohara, Takaji Wakita, Hiromichi Yonekawa, Choji Taya, Izumu Saito
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Patent number: 6201166Abstract: Transgenic mice are described which serve as a model for Hepatitis C infection. The transgenic mice contain, in their germline and somatic cells, the Hepatitis C viral fragment CN2, N24 or CR under the control of a Cre-loxP switch-expression system. Administration of Cre to the mice results in a phenotype of increased serum GTP levels, emergence of acidophilic bodies in the liver, exfoliation of hepatic cells, hypertrophy and hyperplasia of Kupffer's cells, and conglomeration of lymphocytes.Type: GrantFiled: January 21, 1999Date of Patent: March 13, 2001Assignee: Tokyo Metropolitan Institute of Medical ScienceInventors: Michinori Kohara, Takaji Wakita, Hiromichi Yonekawa, Choji Taya, Izumu Saito
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Patent number: 6063904Abstract: A method for extracellularly producing an ectoprotein of hepatitis C virus comprises the steps of cultivating a transformant which is transformed with an expression vector containing a DNA fragment coding for the ectoprotein of hepatitis C virus and recovering the ectoprotein of hepatitis C virus extracellularly produced by the transformant. The protein originated from the E1 region prepared by the method can be used as a material for preparing a vaccine for preventing HCV infection. In addition, a diagnostic agent containing the protein is useful for the detection of an HCV antibody or the confirmation of the presence thereof in sera or the like. In other words, the protein of the present invention permits the diagnosis of C type hepatitis in high specificity and sensitivity.Type: GrantFiled: August 3, 1998Date of Patent: May 16, 2000Assignee: Japan as represented by the Director General of the Agency of National Institute of HealthInventors: Tatsuo Miyamura, Izumu Saito, Yoshiharu Matsuura, Yoshikazu Honda, Makoto Seki
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Patent number: 5830691Abstract: A method for extracellularly producing an ectoprotein of hepatitis C virus comprises the steps of cultivating a transformant which is transformed with an expression vector containing a DNA fragment coding for the ectoprotein of hepatitis C virus and recovering the ectoprotein of hepatitis C virus extracellularly produced by the transformant. The protein originated from the E1 region prepared by the method can be used as a material for preparing a vaccine for preventing HCV infection. In addition, a diagnostic agent containing the protein is useful for the detection of an HCV antibody or the confirmation of the presence thereof in sera or the like. In other words, the protein of the present invention permits the diagnosis of C type hepatitis in high specificity and sensitivity.Type: GrantFiled: April 24, 1996Date of Patent: November 3, 1998Assignee: Japan as respresented by the Director General of the Agency of National Institute of HealthInventors: Tatsuo Miyamura, Izumu Saito, Yoshiharu Matsuura, Yoshikazu Honda, Makoto Seki
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Patent number: 5817492Abstract: An animal cell is co-transfected with both a recombinant DNA viral vector which bears a promoter, a recombinase gene and a poly(A) sequence and a recombinant DNA viral vector which bears two recombinase-recognizing sequences and which further bears an origin of replication, a promoter, a foreign gene and a poly(A) sequence, each of which is located between the two recombinase-recognizing sequences. Thereafter, in the co-transfected animal cell, a DNA fragment containing the origin of replication, promoter, foreign gene and poly(A) sequence is excised from the vector by the action of a recombinase expressed in the another vector. The DNA fragment forms a circular DNA molecule which autonomously replicates in the co-transfected animal cell due to the origin of replication, whereby the foreign gene is continuously expressed.Type: GrantFiled: August 30, 1995Date of Patent: October 6, 1998Assignee: Sumitomo Pharmaceuticals Company, Ltd.Inventors: Izumu Saito, Yumi Kanegae