Patents by Inventor J. Aquiles Sanchez

J. Aquiles Sanchez has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • REAGENTS FOR IMPROVING PCR ACCURACY
    Publication number: 20220090216
    Abstract: Provided herein are reagents for improving PCR accuracy.
    Type: Application
    Filed: September 28, 2021
    Publication date: March 24, 2022
    Inventors: John RICE, Lawrence WANGH, Arthur H. REIS, JR., Kenneth PIERCE, Cristina HARTSHORN, J. Aquiles SANCHEZ, Stephen VAN HOOSER, Skye FISHBEIN
  • Reagents for improving PCR accuracy
    Patent number: 11162146
    Abstract: Provided herein are reagents for improving PCR accuracy.
    Type: Grant
    Filed: April 1, 2019
    Date of Patent: November 2, 2021
    Assignee: Brandeis University
    Inventors: John Rice, Lawrence Wangh, Arthur H. Reis, Jr., Kenneth Pierce, Cristina Hartshorn, J. Aquiles Sanchez, Stephen Van Hooser, Skye Fishbein
  • Kits and reaction mixtures for analyzing single-stranded nucleic acid sequences
    Patent number: 10570462
    Abstract: Provided herein are kits for performing for nucleic acid sequences.
    Type: Grant
    Filed: May 27, 2016
    Date of Patent: February 25, 2020
    Assignee: Brandeis University
    Inventors: Lawrence J. Wangh, John E. Rice, J. Aquiles Sanchez, Arthur H. Reis, Jr.
  • REAGENTS FOR IMPROVING PCR ACCURACY
    Publication number: 20190292610
    Abstract: Provided herein are reagents for improving PCR accuracy.
    Type: Application
    Filed: April 1, 2019
    Publication date: September 26, 2019
    Inventors: John RICE, Lawrence Wangh, Arthur H. Reis, JR., Kenneth Pierce, Cristina Hartshorn, J. Aquiles Sanchez, Stephen Van Hooser, Skye Fishbein
  • Methods for sequential DNA amplification and sequencing
    Patent number: 9745624
    Abstract: Homogenous detection during or following PCR amplification, preferably LATE-PCR, utilizing fluorescent DNA dye and indirectly excitable labeled primers and probes, improves reproducibility and quantification. Low-temperature homogeneous detection during or following non-symmetric PCR amplification, preferably LATE-PCR, utilizing fluorescent DNA dye and indirectly excitable labeled mismatch-tolerant probes permits analysis of complex targets. Sequencing sample preparation methods following LATE-PCR amplifications reduce complexity and permit “single-tube” processing.
    Type: Grant
    Filed: August 1, 2012
    Date of Patent: August 29, 2017
    Assignee: Brandeis University
    Inventors: Lawrence J. Wangh, John E. Rice, J. Aquiles Sanchez, Kenneth E. Pierce, Jesse Salk, Arthur H. Reis, Cristina Hartshorn
  • Late-PCR
    Patent number: 9476092
    Abstract: A non-symmetric polymerize chain reaction (PCR) amplification method employing a limiting primer in low concentration whose concentration-adjusted melting point at least equals, and preferably exceeds, that of the excess primer, the latter in turn not being more than 25° C. below the melting temperature of the amplicon. Assays employing such amplification and labeled hybridization probes, including assays that include a detection step following primer extension or a low-temperature probe, or both. Kits for performing such assays and primer or primer-and-probe sets for performing the foregoing amplifications and assays.
    Type: Grant
    Filed: January 4, 2013
    Date of Patent: October 25, 2016
    Assignee: Brandeis University
    Inventors: Lawrence J. Wangh, Kenneth Pierce, Cristina Hartshorn, John Rice, J. Aquiles Sanchez
  • Kits and Reaction Mixtures for Analyzing Single-Stranded Nucleic Acid Sequences
    Publication number: 20160258001
    Abstract: Provided herein are kits for performing for nucleic acid sequences.
    Type: Application
    Filed: May 27, 2016
    Publication date: September 8, 2016
    Inventors: Lawrence J. Wangh, John E. Rice, J. Aquiles Sanchez, Arthur H. Reis, JR.
  • Methods, kits and reaction mixtures for analyzing single-stranded nucleic acid sequences
    Patent number: 9353407
    Abstract: Provided herein are fluorescence detection methods for nucleic acid sequences and to kits for performing such methods.
    Type: Grant
    Filed: October 21, 2010
    Date of Patent: May 31, 2016
    Assignee: Brandeis University
    Inventors: Lawrence Wangh, John Rice, J. Aquiles Sanchez, Arthur Reis
  • Detecting nucleic acid variations within populations of genomes
    Patent number: 9169514
    Abstract: This disclosure relates to amplification and detection of a rare variant or variants of a DNA sequence in an abundant variant of the sequence, such as detection of a low-level somatic mutations and minority alleles in an excess of normal nucleic acid target sequences.
    Type: Grant
    Filed: December 1, 2011
    Date of Patent: October 27, 2015
    Assignee: Brandeis University
    Inventors: Yanwei Jia, J. Aquiles Sanchez, John E. Rice, Lawrence J. Wangh
  • REAGENTS FOR IMPROVING PCR ACCURACY
    Publication number: 20140206564
    Abstract: Provided herein are reagents for improving PCR accuracy.
    Type: Application
    Filed: January 23, 2014
    Publication date: July 24, 2014
    Applicant: Brandeis University
    Inventors: John Rice, Lawrence Wangh, Arthur H. Reis, JR., Kenneth Pierce, Cristina Hartshorn, J. Aquiles Sanchez, Stephen Van Hooser, Skye Fishbein
  • DETECTION OF SEQUENCE VARIANTS IN THE HUMAN EPIDERMAL GROWTH FACTOR RECEPTOR (EGFR) GENE
    Publication number: 20140147846
    Abstract: Provided herein are methods for detecting and identifying sequence variants in the human epidermal growth factor receptor (EGFR) gene, and compositions and kits for performing such methods. In particular, nucleic acid amplification and fluorescence detection methods are provided for the detection and identification of EGFR sequence variants.
    Type: Application
    Filed: May 25, 2012
    Publication date: May 29, 2014
    Applicant: BRANDEIS UNIVERSITY
    Inventors: Lawrence J. Wangh, Shana Marie Tetrault, J. Aquiles Sanchez
  • DETECTING NUCLEIC ACID VARIATIONS WITHIN POPULATIONS OF GENOMES
    Publication number: 20140024033
    Abstract: This disclosure relates to amplification and detection of a rare variant or variants of a DNA sequence in an abundant variant of the sequence, such as detection of a low-level somatic mutations and minority alleles in an excess of normal nucleic acid target sequences.
    Type: Application
    Filed: December 1, 2011
    Publication date: January 23, 2014
    Applicant: BRANDEIS UNIVERSITY
    Inventors: Yanwei Jia, J. Aquiles Sanchez, John E. Rice, Lawrence J. Wangh
  • COMPOSITIONS AND METHODS FOR NUCLEIC ACID BASED DIAGNOSTIC ASSAYS
    Publication number: 20130295570
    Abstract: The present invention relates to compositions and methods for nucleic acid based diagnostic assays. In particular, the present invention provides probes and non-amplifiable controls for asymmetric PCR and other amplification modalities. In some embodiments, the present invention provides probe design criteria for probes for use in amplification/detection assays. Further embodiments of the present invention provide non-amplifiable controls for use in generating reference probe signal ratios in amplification detection assays.
    Type: Application
    Filed: October 7, 2011
    Publication date: November 7, 2013
    Applicant: BRANDEIS UNIVERSITY
    Inventors: Lawrence J. Wangh, J. Aquiles Sanchez
  • LATE-PCR
    Publication number: 20130210656
    Abstract: A non-symmetric polymerise chain reaction (PCR) amplification method employing a limiting primer in low concentration whose concentration-adjusted melting point at least equals, and preferably exceeds, that of the excess primer, the latter in turn not being more than 25° C. below the melting temperature of the amplicon. Assays employing such amplification and labeled hybridization probes, including assays that include a detection step following primer extension or a low-temperature probe, or both. Kits for performing such assays and primer or primer-and-probe sets for performing the foregoing amplifications and assays.
    Type: Application
    Filed: January 4, 2013
    Publication date: August 15, 2013
    Applicant: Brandeis University
    Inventors: Lawrence J. Wangh, Kenneth Pierce, Cristina Hartshorn, John Rice, J. Aquiles Sanchez
  • Late-PCR
    Patent number: 8367325
    Abstract: A non-symmetric polymerise chain reaction (PCR) amplification method employing a limiting primer in low concentration whose concentration-adjusted melting point at least equals, and preferably exceeds, that of the excess primer, the latter in turn not being more than 25° C. below the melting temperature of the amplicon. Assays employing such amplification and labeled hybridization probes, including assays that include a detection step following primer extension or a low-temperature probe, or both. Kits for performing such assays and primer or primer-and-probe sets for performing the foregoing amplifications and assays.
    Type: Grant
    Filed: February 2, 2007
    Date of Patent: February 5, 2013
    Assignee: Brandeis University
    Inventors: Lawrence J. Wangh, Kenneth Pierce, Cristina Hartshorn, John Rice, J. Aquiles Sanchez
  • METHODS, KITS AND REACTION MIXTURES FOR ANALYZING SINGLE-STRANDED NUCLEIC ACID SEQUENCES
    Publication number: 20120282611
    Abstract: Provided herein are fluorescence detection methods for nucleic acid sequences and to kits for performing such methods.
    Type: Application
    Filed: October 21, 2010
    Publication date: November 8, 2012
    Applicant: BRANDEIS UNIVERSITY
    Inventors: Lawrence Wangh, John Rice, J. Aquiles Sanchez, Arthur Reis
  • Primers, probes and methods for nucleic acid amplification
    Publication number: 20120040352
    Abstract: Homogenous detection during or following PCR amplification, preferably LATE-PCR, utilizing fluorescent DNA dye and indirectly excitable labeled primers and probes, improves reproducibility and quantification. Low-temperature homogeneous detection during or following non-symmetric PCR amplification, preferably LATE-PCR, utilizing fluorescent DNA dye and indirectly excitable labeled mismatch-tolerant probes permits analysis of complex targets. Sequencing sample preparation methods following LATE-PCR amplifications reduce complexity and permit “single-tube” processing.
    Type: Application
    Filed: November 10, 2008
    Publication date: February 16, 2012
    Inventors: Lawrence J. Wangh, John Rice, J. Aquiles Sanchez, Kenneth Pierce, Jesse Salk, Arthur Reis, Cristina Hartshorn
  • RT-LATE-PCR
    Publication number: 20110311971
    Abstract: An assay comprising more than one primer pair and more than one detection probe, a low copy number synthetic amplicon corresponding to each of the primer pairs. The assay can detect and distinguish between various sub-types and strains of an influenza virus using any suitable nucleic acid amplification technique. Related kits and methods are also described.
    Type: Application
    Filed: May 9, 2011
    Publication date: December 22, 2011
    Applicant: BRANDEIS UNIVERSITY
    Inventors: Cristina Hartshorn, Kenneth E. Pierce, Arthur Henry Reis, JR., John E. Rice, J. Aquiles Sanchez, Lawrence J. Wangh
  • Detection and analysis of influenza virus
    Patent number: 7972786
    Abstract: An assay comprising more than one primer pair and more than one detection probe, a low copy number synthetic amplicon corresponding to each of the primer pairs. The assay can detect and distinguish between various sub-types and strains of an influenza virus using any suitable nucleic acid amplification technique. Related kits and methods are also described.
    Type: Grant
    Filed: July 6, 2007
    Date of Patent: July 5, 2011
    Assignee: Brandeis University
    Inventors: Cristina Hartshorn, Kenneth E. Pierce, Arthur Henry Reis, Jr., John E. Rice, J. Aquiles Sanchez, Lawrence J. Wangh
  • Primers, probes and methods for nucleic acid amplification
    Patent number: 7632642
    Abstract: Homogenous detection during or following PCR amplification, preferably LATE-PCR, utilizing fluorescent DNA dye and indirectly excitable labeled primers and probes, improves reproducibility and quantification. Low-temperature homogeneous detection during or following non-symmetric PCR amplification, preferably LATE-PCR, utilizing fluorescent DNA dye and indirectly excitable labeled mismatch-tolerant probes permits analysis of complex targets. Sequencing sample preparation methods following LATE-PCR amplifications reduce complexity and permit “single-tube” processing.
    Type: Grant
    Filed: October 17, 2005
    Date of Patent: December 15, 2009
    Assignee: Brandeis University
    Inventors: Lawrence J. Wangh, John Rice, J. Aquiles Sanchez, Kenneth Pierce, Jesse Salk, Arthur Reis, Cristina Hartshorn