Patents by Inventor J. Fred Crawford

J. Fred Crawford has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Methods of determining deficiencies in intracellular levels on cysteine and glutathione
    Patent number: 7279301
    Abstract: The present invention provides a method for determining an antioxidant deficiency in a lymphocyte by determining the level of intracellular cysteine in the lymphocyte by measuring lymphocyte growth in a serum-free cell culture medium comprising cumene hydroperoxide and N-acetyl-L-cysteine (NAC), whereby any antioxidant deficiency correlates with lymphocyte growth in such a cell culture medium.
    Type: Grant
    Filed: September 26, 2005
    Date of Patent: October 9, 2007
    Assignee: Research Development Foundation
    Inventor: J. Fred Crawford
  • Cell culture media containing N-acetyl-L-cysteine and uses thereof
    Patent number: 6949382
    Abstract: The present invention provides cell culture media and methods useful for determining levels of intracellular function of glutathione or cysteine and for providing biochemical analysis of antioxidant function in human lymphocytes.
    Type: Grant
    Filed: October 29, 2003
    Date of Patent: September 27, 2005
    Assignee: Research Development Foundation
    Inventor: J. Fred Crawford
  • Methods of determining deficiencies in intracellular levels of cysteine and glutathione
    Publication number: 20040087023
    Abstract: The present invention provides cell culture media and methods useful for determining levels of intracellular function of glutathione or cysteine and for providing biochemical analysis of antioxidant function in human lymphocytes.
    Type: Application
    Filed: October 29, 2003
    Publication date: May 6, 2004
    Applicant: Research Development Foundation
    Inventor: J. Fred Crawford
  • Methods of determining deficiencies in intracellular levels of cysteine and glutathione
    Patent number: 6709835
    Abstract: The present invention provides cell culture mediums and methods for determining levels of intracellular concentration of glutathione or cysteine in lymphocytes, indicative of nutritional status of an individual.
    Type: Grant
    Filed: December 13, 2001
    Date of Patent: March 23, 2004
    Inventor: J. Fred Crawford
  • Assessment of intracellular cysteine and glutathione concentrations
    Publication number: 20020068270
    Abstract: The present invention provides a cell culture medium and methods useful for determining levels of intracellular function of glutathione or cysteine, and for providing biochemical analysis of antioxidant function in human lymphocytes. The medium of the present invention comprises: a buffered, serum-free solution containing a carbohydrate selected from the group consisting of glucose and a compound biologically capable of producing glucose in the cells, a biologically usable form of pantothenic acid, choline or a biological usable form of a substance capable of producing choline in the cells, inorganic ions comprising chloride, phosphate, calcium, magnesium, potassium, sodium, and iron in a biologically utilizable form, deionized water, and a mitogen in an amount effective to stimulate the lymphocytes being assayed. In addition to the preceding ingredients, the medium effective for measuring glutathione concentration also contains L-Buthionine-[S.R.]-Sulfoximine.
    Type: Application
    Filed: December 13, 2001
    Publication date: June 6, 2002
    Applicant: Research Development Foundation
    Inventor: J. Fred Crawford
  • Biochemical analysis of antioxidant function of lymphocytes in culture
    Patent number: 5985665
    Abstract: The present invention provides a cell culture medium useful for a biochemical analysis of antioxidant function in human lymphocytes, said medium comprising, a buffered, serum-free solution containing the following ingredients: a carbohydrate selected from the group consisting of glucose and a compound biologically capable of producing glucose in the cells, a biologically usable form of pantothenic acid, choline or a biological usable form of a substance capable of producing choline in the cells, inorganic ions comprising chloride, phosphate, calcium, magnesium, potassium, sodium, and iron in a biologically utilizable form, cumene hydroperoxide, deionized water, and a mitogen in an amount effective to stimulate the lymphocytes being assayed; said buffered, serum-free solution having a pH from about 6.8 to 7.6, said cell culture medium characterized by being effective to determine nutritional deficiencies, inadequacies, and imbalances and to biochemically analyze antioxidant function of the lymphocytes.
    Type: Grant
    Filed: June 19, 1996
    Date of Patent: November 16, 1999
    Assignee: Research Development Foundation
    Inventors: J. Fred Crawford, Luke Bucci