Abstract: The present invention relates to methods of identifying multi-finger Zf polypeptides that bind to a sequence of interest. Zf polypeptides identified using the methods described herein can have affinity and specificity for their target sites that is superior to those produced by alternative methods.

Abstract: The present invention relates to non-naturally occurring zinc finger proteins that are selected for binding to a DNA sequence of interest. The non-naturally occurring zinc finger proteins of the present invention are based on the sequence of zinc finger proteins having more than three zinc fingers, such as NRSF, and are capable of binding extended DNA target sequences with high affinity and specificity.

Abstract: The present invention provides methods and compositions for interaction trap assays for detecting protein-protein, protein-DNA, or protein-RNA interactions. The methods and compositions of the invention may also be used to identify agents which may agonize or antagonize a protein-protein, protein-DNA, or protein-RNA interactions. In certain embodiments, the interaction trap system of the invention is useful for screening libraries with greater than 107 members. In other embodiments, the interaction trap system of the invention is used in conjunction with flow cytometry. The invention further provides a means for simultaneously screening a target protein or nucleic acid sequence for the ability to interact with two or more test proteins or nucleic acids.

Abstract: The present invention makes available an interaction trap system (hereinafter “ITS”) which is derived using recombinantly engineered prokaryotic cells.

Abstract: An immobilized biocatalyst suitable for fermenting to produce ethanol is prepared by mixing a biocatalyst such as a microorganism with a reaction product of a homogeneous dispersion of an anionic polysaccharide such as alginate and a cationic polymer such as polyethyleneimine, and combining the resultant dispersion with an oil phase to form beads. A surtactant may be present when combining the dispersion with the oil phase. A water soluble-oil insoluble curing powder includinga salt of a multivalent cation such as calcium chloride is mixed with the beads in the oil phase to gell and dehydrate the beads and to prevent the beads from adhering to one another until individual bead surfaces become hardened.

Abstract: A surgeon's glove which is free of mold release powder of mineral origin. The glove is "talc free" and contains on a surface a lipo compound and a surfactant for such lipo compound. The glove is manufactured by a process which includes coating a glove form with a coagulant containing the lipo compound and its surfactant in a dispersion which stay with the glove after it is stripped from the mold providing a mold release surface for such glove.

Abstract: A powder free surgeon's glove of a material such as latex rubber, which has covalently bonded to its outer surface a halogen resistant layer, such as silicone. The inner surface of the glove has been halogen treated for smooth nontacky donning characteristics.

Abstract: A hypoallergenic surgeon's glove with an allergenic elastomeric support glove, such as natural latex, has secured to its inner surface by covalent chemical bonds a barrier glove of a nonallergenic elastomer, such as silicone. Sharp angular flexing in the knuckle area, as well as stretching and snapping during donning, does not crack the barrier glove nor delaminate it from the support glove. This hypoallergenic glove requires only about 1/3 the amount of lubricating donning powder in a natural latex glove. In another example, a slip resistant glove, such as silicone, is secured by covalent chemical bonds to an external surface of the support glove.