Abstract: A method of increasing production of a methylmalonyl-CoA derivative in a bacterium is disclosed that includes providing a bacterium that produces a methylmalonyl-CoA derivative in which methylmalonyl-CoA mutase function has been inhibited and culturing the bacterium in a medium containing an effective amount of methylmalonyl-CoA or a precursor thereof to increase the production of the methylmalonyl-CoA derivative by the bacterium. The production of the methylmalonyl-CoA derivative is increased when compared to production of the methylmalonyl-CoA derivative by the bacterium when cultured in a medium without the effective amount of methylmalonyl-CoA or a precursor thereof.

Abstract: A process of increasing the cellular production of biologically active compounds is provided. The process is particularly useful for increasing antibiotic production by bacterial cells. The process includes the step of inhibiting the activity of methylmalonyl-CoA mutase.

Abstract: A process of increasing the cellular production of biologically active compounds is provided. The process is particularly useful for increasing antibiotic production by bacterial cells. The process includes the step of inhibiting the activity of methylmalonyl-CoA mutase.

Abstract: A method for obtaining improved yields of isoflavones from spent fermentation beer produced by Actinomycetes grown on leguminous plant materials is demonstrated. This method involves conversion of isoflavone biotransformation products to isoflavone aglycones followed by isolation of the isoflavone aglycones. Formation of the biotransformation products had previously limited the production of isoflavone aglycones in these Actinomycete fermentations.

Abstract: The present invention relates to a method of improving the strain used for the production of erythromycin through the disruption of the melA gene.

Abstract: We have developed a new gene transfer system for extreme thermophiles of the genus Thermus, including Thermus flavus., using a chromosomal gene, and a thermostable derivative of the kanamycin-resistance gene (kantr2). A plasmid mediated gene-replacement process is used to insert it into the chromosome resulting in the production of Leu−Kmr transformants. This system not only allows stable, single-copy gene insertion into the chromosome of an extreme thermophile, but can be used in the thermo-genetic process described here to generate thermo-stabilized enzymes and proteins for industrial processes. This host-vector environment makes it possible to generate further thermo-stabilizing mutations in the kan gene beyond those levels previously reported.

Abstract: The instant disclosure teaches transposons and methods of the instant invention for making Protein Fusions by rapid, random shuffling of protein domains to produce novel protein fusions. This system is generally applicable to production of multifunctional chimeric proteins in vivo and in vitro. The methods and constructs of the instant invention can be used to randomly create both carboxy- and amino-terminal protein fusions in vivo. The methods and contructs of the instant invention are useful in the development of a protein domain library, in the construction of multifunctional enzymes, and in the accelerated evolution of new enzymatic activities.

Abstract: The present invention provides isolated and purified polypeptides that increase antibiotic production, polynucleotides that encode those polypeptides, expression vectors containing those polynucleotides, host cells transformed with those expression vectors, a process of making the polypeptides using those polynucleotides and vectors, and processes using those polypeptides and polynucleotides.

Abstract: We have developed a new gene transfer system for extreme thermophiles of the genus Thermus, including Thermus flavus., using a chromosomal gene, and a thermostable derivative of the kanamycin-resistance gene (kan.sup.tr2). A plasmid mediated gene-replacement process is used to insert it into the chromosome resulting in the production of Leu.sup.- Km.sup.r transformants. This system not only allows stable, single-copy gene insertion into the chromosome of an extreme thermophile, but can be used in the thermo-genetic process described here to generate thermo-stabilized enzymes and proteins for industrial processes. This host-vector environment makes it possible to generate further thermo-stabilizing mutations in the kan gene beyond those levels previously reported.

Abstract: We have developed a new gene transfer system for extreme thermophiles of the genus Thermus, including Thermus flavus., using a chromosomal gene, and a thermostable derivative of the kanamycin-resistance gene (kan.sup.tr2). A plasmid mediated gene-replacement process is used to insert it into the chromosome resulting in the production of Leu.sup.- Km.sup.r transformants. This system not only allows stable, single-copy gene insertion into the chromosome of an extreme thermophile, but can be used in the thermo-genetic process described here to generate thermo-stabilized enzymes and proteins for industrial processes. This host-vector environment makes it possible to generate further thermo-stabilizing mutations in the kan gene beyond those levels previously reported.

Abstract: The present invention provides a process of preparing the isoflavone genistein. Genistein is prepared by fermenting the bacteria Saccharopolyspora erythraea in the presence of a soy-based substrate. A process of recovering genistein from a bacterial fermentation medium using alkaline, organic solvent extraction is also provided.

Abstract: Novel 6-deoxyerythromycin derivatives are disclosed, as are methods for their preparation and use as antiinfective agents. The compounds of the invention include 6-deoxyerythromycins and 6-deoxy-15-norerythromycins which are represented by the structural formula ##STR1## in which R.sub.1 is selected from OH and H, and R.sub.2 and R.sub.3 are independently selected from H and CH.sub.3, as well as the pharmaceutically acceptable salts and esters of the above compounds. Additionally disclosed are genetically modified microorganisms which produce the compounds of the invention and means for the preparation of those microorganisms.