Patents by Inventor Jérôme Larghero

Jérôme Larghero has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 8361796
    Abstract: The present invention is directed to a method for the in vitro preparation of cardiovascular progenitors cells from mammalian embryonic stem cells (ES cells) or mammalian embryonic-like state cells, preferably from primate, wherein said method comprises the use of the CD15 (SSEAI) marker as a positive cardiovascular progenitors differentiation marker. The present invention also claimed the use of a receptor tyrosine kinase inhibitor, particularly the SU5402 or SU11248 in association with the BMP2 for improving the efficiency of the desired differentiation. The present invention is also directed to the use of platelet lysate as foetal animal serum substitute in a culture medium intended to the proliferation or propagation of primate ES cells maintaining their pluripotency feature. Derived compositions or kits in relation with the claimed methods or product obtainable by the claimed methods form also part of the present invention.
    Type: Grant
    Filed: March 10, 2009
    Date of Patent: January 29, 2013
    Assignees: Assistance Publique—Hopitaux de Paris, Institut National de la Sante et de la Recherche Medicale (Inserm), Universite Paris Descartes, Universite Paris Diderot Paris 7
    Inventors: Philippe Menasche, Michel Puceat, Jérôme Larghero, Guillaume Blin, David Nury, Sonia Stefanovic
  • Publication number: 20110189772
    Abstract: The invention relates to a method for using a dual-compartment bioreactor that comprises a flexible pouch (1) with two compartments (2, 3) separated by a filter (4), as well as at least one inlet opening (5) in communication with one of the compartments (2) and at least one outlet opening (6) in communication with the other compartment (3), wherein said method comprises the implementation thereof in biological processing equipment particularly for washing or converting a tissue or biological product, characterised in that the method comprises, for a tissue dissociation, using said bioreactor as a digestion chamber and manually kneading the flexible pouch (1) for dissociating the tissues during a digestion step in which a digestion solution containing collagenase flows between the inlet (5) and outlet (6) openings of the pouch.
    Type: Application
    Filed: May 13, 2009
    Publication date: August 4, 2011
    Inventors: Marc Benbunan, Pirre Cattan, Jérôme Larghero
  • Publication number: 20110014691
    Abstract: The present invention is directed to a method for the in vitro preparation of cardiovascular progenitors cells from mammalian embryonic stem cells (ES cells) or mammalian embryonic-like state cells, preferably from primate, wherein said method comprises the use of the CD15 (SSEAI) marker as a positive cardiovascular progenitors differentiation marker. The present invention also claimed the use of a receptor tyrosine kinase inhibitor, particularly the SU5402 or SU11248 in association with the BMP2 for improving the efficiency of the desired differentiation. The present invention is also directed to the use of platelet lysate as foetal animal serum substitute in a culture medium intended to the proliferation or propagation of primate ES cells maintaining their pluripotency feature. Derived compositions or kits in relation with the claimed methods or product obtainable by the claimed methods form also part of the present invention.
    Type: Application
    Filed: March 10, 2009
    Publication date: January 20, 2011
    Applicants: ASSISTANCE PUBLIQUE-HOPITAUX DE PARIS, INSTITUT NATIONAL DE LA SANTE ET DE LA RECHERCHE MEDICALE (INSERM), UNIVERSITE PARIS DECARTES, UNIVERSITE PARIS DIDEROT PARIS 7, UNIVERSITE MONTPELLIER 2 SCIENCES ET TECHNIQUES
    Inventors: Philippe Menasche, Michel Puceat, Jérôme Larghero, Guillaume Blin, David Nury, Sonia Stefanovic