Abstract: The present disclosure provides a pet food, comprising: a) a pet food core, b) one or more active layers surrounding all, or a portion of, the pet food core, and c) an outer layer at least partially surrounding the active layer, wherein the outer layer comprises a fat and/or a pallatant, wherein the active layer may comprise one or more of an antioxidant compound and a pro-oxidant compound. The active layer may be enriched with the pro-oxidant mineral compared to the pet food core, and/or the pet food core and the active layer may have different total concentrations of antioxidant compounds and/or the pet food core and the active layer may comprise different antioxidant compounds and/or the same antioxidant compounds in different concentrations.

Abstract: The invention relates to a method comprising the following steps: a) use of a support which has at least two different microparticle populations immobilized thereon; b) measuring the fluorescence of the support from step a) with an optical resolution 1, said resolution 1 permitting differentiation of microparticle singlets, doublets, triplets, multiplets and monolayers and determination of the localized position of individual immobilized microparticles; c) contacting the support from step a) with the sample to be analyzed; d) performing at least one additional measurement of the fluorescence of the support during or after contacting in accordance with step c) with a resolution 2; e) assigning the fluorescence values measured with resolution 2 to the individual microparticle singlets, doublets, triplets, multiplets and monolayers locally identified on the support in accordance with step b) and assigned to a particular acceptor molecule population; f) determining the change in fluorescence.