Patents by Inventor Jörg Siebenmorgen

Jörg Siebenmorgen has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 11892618
    Abstract: A light beam shaping arrangement for a light microscope has a first and a second liquid crystal region or lifting micromirror region, each of which has a plurality of independently switchable liquid crystal elements or mirrors with which a phase of incident light is changeable in a settable manner, an input-/output-coupling polarization beam splitter, a polarization beam splitter arranged between the input-/output-coupling polarization beam splitter and the liquid crystal regions or lifting micromirror regions such that the polarization beam splitter separates the light coming from the input-/output-coupling polarization beam splitter in a polarization-dependent manner into a first partial beam.
    Type: Grant
    Filed: April 11, 2019
    Date of Patent: February 6, 2024
    Assignee: Carl Zeiss Microscopy GmbH
    Inventors: Jörg Siebenmorgen, Ingo Kleppe, Ralf Netz
  • Patent number: 11796782
    Abstract: An optics arrangement for flexible multi-color illumination for a light microscope includes an acousto-optical tunable filter (“AOTF”). The AOTF is set up to diffract two light components from incident illumination light into different order-of-diffraction directions. The two light components differ in their wavelengths and polarizations. Alternatively, an electro-optical modulator (“EOM”) can be used, with which two temporally successive light components of different wavelengths are set to different polarization directions. A polarization beam splitter separates the two light components of different wavelengths and polarizations into reflection light, which is reflected at the polarization beam splitter, and transmission light, which is transmitted at the polarization beam splitter. A light structuring apparatus imprints different structures onto the transmission light and the reflection light.
    Type: Grant
    Filed: April 11, 2019
    Date of Patent: October 24, 2023
    Assignee: CARL ZEISS MICROSCOPY GMBH
    Inventors: Jörg Siebenmorgen, Ingo Kleppe, Ralf Netz
  • Publication number: 20230266246
    Abstract: An apparatus and method for structured illumination microscopy, having an illumination beam path for irradiating a sample with excitation light with a two-dimensional illumination pattern at angles greater than the angle for total internal reflection. The illumination beam path has an illumination objective used to irradiate the sample. A first separation device for separating the excitation light in a first linear coordinate direction in a pupil plane and a displacement device for laterally displacing the illumination pattern in a sample plane, having a detection beam path containing at least one microscope objective for guiding emission light to a camera. The emission light is emitted by the sample as a consequence of the irradiation by the excitation light. A camera for recording images of the sample, has a control unit for calculating microscopic images of the sample using partial images of the sample recorded for different positions of the illumination pattern in the sample plane.
    Type: Application
    Filed: February 6, 2023
    Publication date: August 24, 2023
    Inventors: Joerg SIEBENMORGEN, Ingo KLEPPE, Ralf NETZ
  • Publication number: 20230251480
    Abstract: Techniques an apparatuses for localizing objects of a sample that emit detection radiation are disclosed. At least one light sheet is produced in a light sheet plane and a region of the sample to be imaged is illuminated with the light sheet and detection radiation from the sample is spatially resolved in a detection plane. A light sheet is produced that has, in the light sheet plane, a non-illuminated light sheet section delimited by an illuminated light sheet section or is produced sequentially in the light sheet plane, where a non-illuminated light sheet section remains between the sequentially produced light sheets. An object to be localized is present in the non-illuminated region, and a position of the object is captured if it leaves the non-illuminated region and is excited to emit the detection radiation by one of the light sheets delimiting the non-illuminated region.
    Type: Application
    Filed: February 9, 2023
    Publication date: August 10, 2023
    Inventors: Thomas Kalkbrenner, Jörg Siebenmorgen
  • Patent number: 11719922
    Abstract: A single plane illumination microscope having an illumination optical system for illuminating a sample located on a sample carrier in a medium, and which is parallel to a planar reference surface. The sample is illuminated by a light sheet via an illumination light path. A detection optical system has a detection beam path. The optical axes of the illumination and detection optical systems each define an angle that is not equal to zero degrees along with the normal to the reference surface. A barrier layer system includes at least one layer of a given material having a given thickness and separates the medium from the illumination and detection optical systems. A base area of the barrier layer system is in contact with the region that is accessible for illumination and detection activities, said base area running parallel to the reference surface.
    Type: Grant
    Filed: April 14, 2021
    Date of Patent: August 8, 2023
    Assignee: CARL ZEISS MICROSCOPY GMBH
    Inventors: Thomas Kalkbrenner, Ralf Netz, Helmut Lippert, Joerg Siebenmorgen
  • Publication number: 20230236400
    Abstract: A method, an arrangement for microscopy and a microscope for three-dimensional imaging in microscopy, in which aberrations of a specimen detection radiation coming from a specimen are corrected in a detection beam path by means of a correction element and the corrected specimen detection radiation is captured in a spatially resolved form. The inventions are distinguished by the fact that a best-possible correction setting of the correction element, with which aberrations occurring at the time are reduced as much as possible, is determined; and, on the basis of the best-possible correction setting, a flawed correction setting is determined, a setting with which aberrations occurring lead to an asymmetric point spread function of the specimen detection radiation.
    Type: Application
    Filed: January 25, 2023
    Publication date: July 27, 2023
    Inventors: Thomas Kalkbrenner, Joerg Siebenmorgen
  • Patent number: 11635606
    Abstract: An optical lens for use in a media feed device, having a first lens surface and a second lens surface, wherein the first lens is provided to be facing an object to be observed and the second lens surface is provided to be facing away from the object to be observed. At least one channel opening onto the first lens surface is present, wherein the at least one channel runs through the optical lens and at least one section of a media line is formed in the at least one, channel or, if a plurality of channels are formed, at least one of the plurality of channels opens up outside a highest point in alignment with a line that is vertical with respect to the first lens surface and to the surface of the earth.
    Type: Grant
    Filed: September 28, 2018
    Date of Patent: April 25, 2023
    Assignee: CARL ZEISS MICROSCOPY GMBH
    Inventors: Saskia Pergande, Peter Schacht, Andreas Möbius, Jörg Siebenmorgen, Thomas Kalkbrenner
  • Patent number: 11609414
    Abstract: Method for calibrating a phase mask in a beam path of an optical device with the steps: the phase mask is actuated successively with different patterns of grey levels, wherein a first grey level of a first quantity of segments remains constant and a second grey level of a second quantity of segments is varied from one pattern to the next, light of the optical device impinges on the phase mask, at least one part of the total intensity of the light in the beam path is measured downstream of the phase mask for the different patterns, and a characteristic of the measured intensity is obtained in dependence on the second grey level, a relationship between the second grey level and a phase shift, being imprinted by the phase mask, is obtained from the characteristic and an actuation of the phase mask is calibrated based on the obtained relationship.
    Type: Grant
    Filed: June 18, 2019
    Date of Patent: March 21, 2023
    Assignee: Carl Zeiss Microscopy GmbH
    Inventors: Toni Eichelkraut, Jörg Siebenmorgen
  • Patent number: 11555991
    Abstract: A method for illuminating samples in microscopic imaging methods, wherein a number m of different wavelengths ?i, with m>I and i=I, . . . , m, is selected for the illumination. For each of the wavelengths ?i a target phase function ??i(x, y, ?i) is predefined, wherein x and y denote spatial coordinates in a plane perpendicular to an optical axis z and each target phase function ??i(x, y, ?i) is effective only for the corresponding wavelength ?i. The target phase functions ??i are predefined depending on the structure of the sample and/or the beam shape and/or illumination light structure to be impressed on the light used for illumination. A total phase mask is then produced which realises all target phase functions ??i(x, y, ?i). This total phase mask is then illuminated simultaneously or successively with coherent light of wavelengths ?i such that the predefined structure of the illumination light is generated in the region of the sample.
    Type: Grant
    Filed: May 24, 2019
    Date of Patent: January 17, 2023
    Assignee: CARL ZEISS MICROSCOPY GMBH
    Inventors: Joerg Siebenmorgen, Ralf Netz
  • Patent number: 11550135
    Abstract: An optical arrangement for light beam shaping in a light microscope has a first and a second liquid crystal region, each of which has a plurality of independently switchable liquid crystal elements with which a phase of incident light is changeable in a settable manner. A first polarization beam splitter is arranged in such a way that incident light is split in a polarization-dependent manner into reflection light, which is reflected in the direction of the first liquid crystal region, and transmission light, which is transmitted in the direction of the second liquid crystal region. The first or a second polarization beam splitter is arranged such that the reflection light and transmission light are combined onto a common beam path after phase modulation by means of the liquid crystal regions.
    Type: Grant
    Filed: April 11, 2019
    Date of Patent: January 10, 2023
    Assignee: CARL ZEISS MICROSCOPY GMBH
    Inventors: Jörg Siebenmorgen, Ingo Kleppe, Ralf Netz
  • Publication number: 20220276478
    Abstract: A method for light sheet microscope examination of a specimen, the specimen being illuminated with a light sheet by an illumination objective, and a light sheet microscope for performing the method. Light emitted by the specimen is imaged onto an area-type detector by a detection objective. An optical axis of the detection objective encloses with an optical axis of the illumination objective an angle that differs from 0° and from 180° and intersects the light sheet in a light sheet plane. The area-type detector records an image. A neural network analyzes the image to determine whether the light sheet plane is located in a focal plane of the detection objective, and/or in which direction along the optical axis the light sheet plane is located from the focal plane, and/or at what distance, measured along the optical axis of the detection objective, the light sheet plane is from the focal plane.
    Type: Application
    Filed: February 25, 2022
    Publication date: September 1, 2022
    Inventors: Manuel AMTHOR, Daniel HAASE, Thomas KALKBRENNER, Jörg SIEBENMORGEN
  • Patent number: 11371927
    Abstract: A method for optically examining a plurality of microscopic samples. The samples are channeled one after the other by means of a flow into at least one flow channel in which the samples advance along a flow direction. The samples are illuminated, and light emitted from the samples is detected and analyzed. A device for carrying out the method in which samples are illuminated in that at least one light sheet with a light sheet plane is directed onto the at least one flow channel, wherein the light sheet is oriented so as to intersect the at least one flow channel in an intersection region, and the normal of the light sheet plane forms an angle differing from null together with the flow direction in the intersection region. The light emitted from the sample is registered by an imaging optical detection unit, and the focal plane of said optical detection unit lies in the intersection region.
    Type: Grant
    Filed: September 25, 2018
    Date of Patent: June 28, 2022
    Assignee: Carl Zeiss Microscopy GmbH
    Inventors: Thomas Kalkbrenner, Joerg Siebenmorgen, Thomas Ohrt
  • Patent number: 11327288
    Abstract: A method is useful for generating an overview image using a high-aperture objective. An optical detection beam path with the objective is provided. The path is transferred into a second operating state via a first depth of field increased to a second depth of field. Detection radiation is captured in the second operating state. Detection radiation coming from the sample space is collected by the objective, guided along the path, and captured by a detector sensitive to detection radiation as an image with a lateral image field, which is the same in the first and second operating states.
    Type: Grant
    Filed: June 4, 2019
    Date of Patent: May 10, 2022
    Assignee: Carl Zeiss Microscopy GmbH
    Inventors: Jakob Haarstrich, Jörg Siebenmorgen, Helge Eggert, Martin Beck
  • Publication number: 20220137382
    Abstract: In a light sheet microscope, a detection device images a sample volume and an illumination device illuminates the sample with a light sheet. A light sheet plane is arranged substantially perpendicularly to the optical imaging axis and an illumination width extends perpendicular to the light sheet plane. A processing device is provided to control setting of the location of the light sheet plane in the sample volume, and recording a plurality of images of the sample volume with different locations of the light sheet plane. For the plurality of images recorded, a spacing, which is measured along the optical imaging axis, between adjacent locations of the light sheet plane is greater than or equal to half the illumination width, but not greater than the illumination width. Intermediate images for the stack of images in additional locations of the light sheet plane in the sample volume are computed.
    Type: Application
    Filed: October 28, 2021
    Publication date: May 5, 2022
    Inventors: Ralf NETZ, Joerg SIEBENMORGEN
  • Patent number: 11314068
    Abstract: The invention relates to an illumination apparatus for a microscope, a microscope and a method for operating the illumination apparatus. The illumination apparatus has a sample space for holding a sample that is to be illuminated, and at least one laser light source. An objective for the directional emission of laser radiation of a first wavelength along a first optical axis that is directed into the sample space, and with a cover of the sample space by which the sample space is delimited at least on one of its sides. The cover further has a layer that is either impenetrable for the laser radiation over a blocking angle range of the illumination angle and is transmissive for radiation of a second wavelength over a transmitted light angle range, or has a controllable layer that, in a first control state, is transparent for radiation of the second wavelength and, in a second control state, is impenetrable for the laser radiation of the first wavelength.
    Type: Grant
    Filed: September 18, 2019
    Date of Patent: April 26, 2022
    Assignee: Carl Zeiss Microscopy GmbH
    Inventors: Thomas Kalkbrenner, Jakob Haarstrich, Jörg Siebenmorgen, Andreas Möbius
  • Patent number: 11300770
    Abstract: A method for adjusting a specimen holder in the beam path of a microscope, in which at least one beam of an illumination radiation is directed onto the specimen holder; a component of the illumination radiation reflected by the specimen holder is captured by means of a detector and measurement values of the captured illumination radiation are ascertained. A current actual manner of positioning of the specimen holder in relation to the beam path is established depending on the measurement values; the established actual manner of positioning is compared to an intended manner of positioning, and control commands for modifying the actual manner of positioning are produced, the execution of which causes the specimen holder to be moved into the intended manner of positioning.
    Type: Grant
    Filed: June 29, 2017
    Date of Patent: April 12, 2022
    Assignee: Carl Zeiss Microscopy GmbH
    Inventors: Jörg Siebenmorgen, Thomas Kalkbrenner
  • Patent number: 11300771
    Abstract: A method for operating a microscopy arrangement, and a microscopy arrangement, having a first microscope and at least one further microscope, wherein each of the microscopes have a respective optical axis. The respective optical axes do not coincide. The method provides a three-dimensional reference coordinate system being set; a carrier apparatus, that is embodied in the arrangement to receive and hold a specimen carrier is introduced into a specimen plane of the first microscope that is intersected by the optical axis and onto the optical axis of the first microscope; a reference point is set on the optical axis of the first microscope; the carrier apparatus is delivered to the further microscope, wherein the current coordinates of the reference point are continuously captured and compared to the coordinates of the optical axis of the at least one further microscope; and the reference point is brought onto the optical axis of the at least one further microscope.
    Type: Grant
    Filed: August 11, 2020
    Date of Patent: April 12, 2022
    Assignee: Carl Zeiss Microscopy GmbH
    Inventors: Thomas Kalkbrenner, Saskia Pergande, Jörg Siebenmorgen, Helmut Lippert
  • Publication number: 20220066188
    Abstract: The invention relates to a method and an apparatus for light sheet microscopy, in which a sample is illuminated with a light sheet and is observed with a microscope, wherein illumination light is shaped to form the light sheet using a controllable phase mask, wherein the controllable phase mask is controlled to form a phase pattern in which at least first regions and second regions are arranged in alternation, wherein a greater phase angle deviation is impressed on the illumination light in the first regions than in the second regions, and wherein at least the phase angle deviation of the first regions is controlled in a spatially dependent manner for influencing the intensity of the illumination light at different locations of a cross-sectional area of the light sheet. The invention additionally relates to a method and an apparatus for providing illumination light with variable intensity.
    Type: Application
    Filed: August 26, 2021
    Publication date: March 3, 2022
    Inventors: Jörg Siebenmorgen, Thomas Kalkbrenner
  • Patent number: 11231572
    Abstract: An arrangement for TIRF microscopy, having an illumination optical unit with an illumination objective for illuminating a specimen on a specimen carrier in a specimen plane via an illumination beam path. An optical axis of the illumination objective includes an illumination angle that differs from zero with the normal of the specimen plane. A detection optical unit with a detection objective in a detection beam path includes a detection angle that differs from zero between an optical axis thereof and the normal of the specimen plane. A transition element between the specimen carrier and both objectives is arranged both in the illumination beam path and in the detection beam path. The transition element corrects aberrations that arise on account of the passage through media with different refractive indices of radiation to be detected and/or radiation for illuminating the specimen.
    Type: Grant
    Filed: February 15, 2018
    Date of Patent: January 25, 2022
    Assignee: Carl Zeiss Microscopy GmbH
    Inventors: Jörg Siebenmorgen, Ralf Netz
  • Patent number: 11163147
    Abstract: An arrangement for microscopy, having an illumination optical unit with an illumination objective for illuminating a specimen on a specimen carrier. An optical axis of the illumination objective lies in a plane which includes an illumination angle that differs from zero with the normal of a specimen plane and the illumination is implemented in the plane. A detection optical unit with a detection objective is located in a detection beam path. The optical axis of the detection objective includes a detection angle that differs from zero with the normal of the specimen plane. The illumination objective and/or the detection objective has an illumination correction element. A meniscus lens is located between the specimen carrier and the illumination and detection objectives being arranged both in the illumination beam path and in the detection beam path.
    Type: Grant
    Filed: June 29, 2017
    Date of Patent: November 2, 2021
    Assignee: Carl Zeiss Microscopy GmbH
    Inventors: Jörg Siebenmorgen, Helmut Lippert, Thomas Kalkbrenner, Ingo Kleppe, Ralf Wolleschensky, Artur Degen, Matthias Wald, Lars-Christian Wittig, Michael Gölles, Wolfgang Singer