Patents by Inventor Jörg Siebenmorgen

Jörg Siebenmorgen has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20220276478
    Abstract: A method for light sheet microscope examination of a specimen, the specimen being illuminated with a light sheet by an illumination objective, and a light sheet microscope for performing the method. Light emitted by the specimen is imaged onto an area-type detector by a detection objective. An optical axis of the detection objective encloses with an optical axis of the illumination objective an angle that differs from 0° and from 180° and intersects the light sheet in a light sheet plane. The area-type detector records an image. A neural network analyzes the image to determine whether the light sheet plane is located in a focal plane of the detection objective, and/or in which direction along the optical axis the light sheet plane is located from the focal plane, and/or at what distance, measured along the optical axis of the detection objective, the light sheet plane is from the focal plane.
    Type: Application
    Filed: February 25, 2022
    Publication date: September 1, 2022
    Inventors: Manuel AMTHOR, Daniel HAASE, Thomas KALKBRENNER, Jörg SIEBENMORGEN
  • Patent number: 11371927
    Abstract: A method for optically examining a plurality of microscopic samples. The samples are channeled one after the other by means of a flow into at least one flow channel in which the samples advance along a flow direction. The samples are illuminated, and light emitted from the samples is detected and analyzed. A device for carrying out the method in which samples are illuminated in that at least one light sheet with a light sheet plane is directed onto the at least one flow channel, wherein the light sheet is oriented so as to intersect the at least one flow channel in an intersection region, and the normal of the light sheet plane forms an angle differing from null together with the flow direction in the intersection region. The light emitted from the sample is registered by an imaging optical detection unit, and the focal plane of said optical detection unit lies in the intersection region.
    Type: Grant
    Filed: September 25, 2018
    Date of Patent: June 28, 2022
    Assignee: Carl Zeiss Microscopy GmbH
    Inventors: Thomas Kalkbrenner, Joerg Siebenmorgen, Thomas Ohrt
  • Patent number: 11327288
    Abstract: A method is useful for generating an overview image using a high-aperture objective. An optical detection beam path with the objective is provided. The path is transferred into a second operating state via a first depth of field increased to a second depth of field. Detection radiation is captured in the second operating state. Detection radiation coming from the sample space is collected by the objective, guided along the path, and captured by a detector sensitive to detection radiation as an image with a lateral image field, which is the same in the first and second operating states.
    Type: Grant
    Filed: June 4, 2019
    Date of Patent: May 10, 2022
    Assignee: Carl Zeiss Microscopy GmbH
    Inventors: Jakob Haarstrich, Jörg Siebenmorgen, Helge Eggert, Martin Beck
  • Publication number: 20220137382
    Abstract: In a light sheet microscope, a detection device images a sample volume and an illumination device illuminates the sample with a light sheet. A light sheet plane is arranged substantially perpendicularly to the optical imaging axis and an illumination width extends perpendicular to the light sheet plane. A processing device is provided to control setting of the location of the light sheet plane in the sample volume, and recording a plurality of images of the sample volume with different locations of the light sheet plane. For the plurality of images recorded, a spacing, which is measured along the optical imaging axis, between adjacent locations of the light sheet plane is greater than or equal to half the illumination width, but not greater than the illumination width. Intermediate images for the stack of images in additional locations of the light sheet plane in the sample volume are computed.
    Type: Application
    Filed: October 28, 2021
    Publication date: May 5, 2022
    Inventors: Ralf NETZ, Joerg SIEBENMORGEN
  • Patent number: 11314068
    Abstract: The invention relates to an illumination apparatus for a microscope, a microscope and a method for operating the illumination apparatus. The illumination apparatus has a sample space for holding a sample that is to be illuminated, and at least one laser light source. An objective for the directional emission of laser radiation of a first wavelength along a first optical axis that is directed into the sample space, and with a cover of the sample space by which the sample space is delimited at least on one of its sides. The cover further has a layer that is either impenetrable for the laser radiation over a blocking angle range of the illumination angle and is transmissive for radiation of a second wavelength over a transmitted light angle range, or has a controllable layer that, in a first control state, is transparent for radiation of the second wavelength and, in a second control state, is impenetrable for the laser radiation of the first wavelength.
    Type: Grant
    Filed: September 18, 2019
    Date of Patent: April 26, 2022
    Assignee: Carl Zeiss Microscopy GmbH
    Inventors: Thomas Kalkbrenner, Jakob Haarstrich, Jörg Siebenmorgen, Andreas Möbius
  • Patent number: 11300770
    Abstract: A method for adjusting a specimen holder in the beam path of a microscope, in which at least one beam of an illumination radiation is directed onto the specimen holder; a component of the illumination radiation reflected by the specimen holder is captured by means of a detector and measurement values of the captured illumination radiation are ascertained. A current actual manner of positioning of the specimen holder in relation to the beam path is established depending on the measurement values; the established actual manner of positioning is compared to an intended manner of positioning, and control commands for modifying the actual manner of positioning are produced, the execution of which causes the specimen holder to be moved into the intended manner of positioning.
    Type: Grant
    Filed: June 29, 2017
    Date of Patent: April 12, 2022
    Assignee: Carl Zeiss Microscopy GmbH
    Inventors: Jörg Siebenmorgen, Thomas Kalkbrenner
  • Patent number: 11300771
    Abstract: A method for operating a microscopy arrangement, and a microscopy arrangement, having a first microscope and at least one further microscope, wherein each of the microscopes have a respective optical axis. The respective optical axes do not coincide. The method provides a three-dimensional reference coordinate system being set; a carrier apparatus, that is embodied in the arrangement to receive and hold a specimen carrier is introduced into a specimen plane of the first microscope that is intersected by the optical axis and onto the optical axis of the first microscope; a reference point is set on the optical axis of the first microscope; the carrier apparatus is delivered to the further microscope, wherein the current coordinates of the reference point are continuously captured and compared to the coordinates of the optical axis of the at least one further microscope; and the reference point is brought onto the optical axis of the at least one further microscope.
    Type: Grant
    Filed: August 11, 2020
    Date of Patent: April 12, 2022
    Assignee: Carl Zeiss Microscopy GmbH
    Inventors: Thomas Kalkbrenner, Saskia Pergande, Jörg Siebenmorgen, Helmut Lippert
  • Publication number: 20220066188
    Abstract: The invention relates to a method and an apparatus for light sheet microscopy, in which a sample is illuminated with a light sheet and is observed with a microscope, wherein illumination light is shaped to form the light sheet using a controllable phase mask, wherein the controllable phase mask is controlled to form a phase pattern in which at least first regions and second regions are arranged in alternation, wherein a greater phase angle deviation is impressed on the illumination light in the first regions than in the second regions, and wherein at least the phase angle deviation of the first regions is controlled in a spatially dependent manner for influencing the intensity of the illumination light at different locations of a cross-sectional area of the light sheet. The invention additionally relates to a method and an apparatus for providing illumination light with variable intensity.
    Type: Application
    Filed: August 26, 2021
    Publication date: March 3, 2022
    Inventors: Jörg Siebenmorgen, Thomas Kalkbrenner
  • Patent number: 11231572
    Abstract: An arrangement for TIRF microscopy, having an illumination optical unit with an illumination objective for illuminating a specimen on a specimen carrier in a specimen plane via an illumination beam path. An optical axis of the illumination objective includes an illumination angle that differs from zero with the normal of the specimen plane. A detection optical unit with a detection objective in a detection beam path includes a detection angle that differs from zero between an optical axis thereof and the normal of the specimen plane. A transition element between the specimen carrier and both objectives is arranged both in the illumination beam path and in the detection beam path. The transition element corrects aberrations that arise on account of the passage through media with different refractive indices of radiation to be detected and/or radiation for illuminating the specimen.
    Type: Grant
    Filed: February 15, 2018
    Date of Patent: January 25, 2022
    Assignee: Carl Zeiss Microscopy GmbH
    Inventors: Jörg Siebenmorgen, Ralf Netz
  • Patent number: 11163147
    Abstract: An arrangement for microscopy, having an illumination optical unit with an illumination objective for illuminating a specimen on a specimen carrier. An optical axis of the illumination objective lies in a plane which includes an illumination angle that differs from zero with the normal of a specimen plane and the illumination is implemented in the plane. A detection optical unit with a detection objective is located in a detection beam path. The optical axis of the detection objective includes a detection angle that differs from zero with the normal of the specimen plane. The illumination objective and/or the detection objective has an illumination correction element. A meniscus lens is located between the specimen carrier and the illumination and detection objectives being arranged both in the illumination beam path and in the detection beam path.
    Type: Grant
    Filed: June 29, 2017
    Date of Patent: November 2, 2021
    Assignee: Carl Zeiss Microscopy GmbH
    Inventors: Jörg Siebenmorgen, Helmut Lippert, Thomas Kalkbrenner, Ingo Kleppe, Ralf Wolleschensky, Artur Degen, Matthias Wald, Lars-Christian Wittig, Michael Gölles, Wolfgang Singer
  • Publication number: 20210247600
    Abstract: An optics arrangement for flexible multi-color illumination for a light microscope comprises an AOTF, which is set up to diffract two light components from incident illumination light into different order-of-diffraction directions, wherein the two light components differ in their wavelengths and polarizations, or an EOM, with which two temporally successive light components of different wavelengths are set to different polarization directions. A polarization beam splitter separates the two light components of different wavelengths and polarizations into reflection light, which is reflected at the polarization beam splitter, and transmission light, which is transmitted at the polarization beam splitter. A light structuring apparatus imprints different structures onto the transmission light and the reflection light. The structured transmission light and the structured reflection light are then recombined by the polarization beam splitter or a further polarization beam splitter onto a common beam path.
    Type: Application
    Filed: April 11, 2019
    Publication date: August 12, 2021
    Applicant: Carl Zeiss Microscopy GmbH
    Inventors: Jörg SIEBENMORGEN, lngo KLEPPE, Ralf NETZ
  • Publication number: 20210239961
    Abstract: A method is useful for generating an overview image using a high-aperture objective. An optical detection beam path with the objective is provided. The path is transferred into a second operating state via a first depth of field increased to a second depth of field. Detection radiation is captured in the second operating state. Detection radiation coming from the sample space is collected by the objective, guided along the path, and captured by a detector sensitive to detection radiation as an image with a lateral image field, which is the same in the first and second operating states.
    Type: Application
    Filed: June 4, 2019
    Publication date: August 5, 2021
    Applicant: Carl Zeiss Microscopy GmbH
    Inventors: Jakob Haarstrich, Jörg Siebenmorgen, Helge Eggert, Martin Beck
  • Publication number: 20210231936
    Abstract: A single plane illumination microscope having an illumination optical system for illuminating a sample located on a sample carrier in a medium, and which is parallel to a planar reference surface. The sample is illuminated by a light sheet via an illumination light path. A detection optical system has a detection beam path. The optical axes of the illumination and detection optical systems each define an angle that is not equal to zero degrees along with the normal to the reference surface. A barrier layer system includes at least one layer of a given material having a given thickness and separates the medium from the illumination and detection optical systems. A base area of the barrier layer system is in contact with the region that is accessible for illumination and detection activities, said base area running parallel to the reference surface.
    Type: Application
    Filed: April 14, 2021
    Publication date: July 29, 2021
    Applicant: Carl Zeiss Microscopy GmbH
    Inventors: Thomas Kalkbrenner, Ralf Netz, Helmut Lippert, Joerg Siebenmorgen
  • Publication number: 20210199942
    Abstract: A method for illuminating samples in microscopic imaging methods, wherein a number m of different wavelengths ?i, with m>I and i=I, . . . , m, is selected for the illumination. For each of the wavelengths ?i a target phase function ??i(x, y, ?i) is predefined, wherein x and y denote spatial coordinates in a plane perpendicular to an optical axis z and each target phase function ??i(x, y, ?i) is effective only for the corresponding wavelength ?i. The target phase functions ??i are predefined depending on the structure of the sample and/or the beam shape and/or illumination light structure to be impressed on the light used for illumination. A total phase mask is then produced which realises all target phase functions ??i(x, y, ?i). This total phase mask is then illuminated simultaneously or successively with coherent light of wavelengths ?i such that the predefined structure of the illumination light is generated in the region of the sample.
    Type: Application
    Filed: May 24, 2019
    Publication date: July 1, 2021
    Applicant: Carl Zeiss Microscopy GmbH
    Inventors: Joerg SIEBENMORGEN, Ralf NETZ
  • Publication number: 20210191097
    Abstract: An illumination apparatus for illuminating a sample plane and a sample that is optionally arranged therein along an illumination beam path includes: a first light source (for outputting light of at least one first wavelength (?photo), a second light source for outputting light of at least one second wavelength (?exc), and a diffraction grating in the illumination beam path between the first and second light sources and the sample plane. Light of the first wavelength (?photo) is not diffracted by the diffraction grating, and light of the second wavelength (?exc) is diffracted due to the effect of the diffraction grating. The illumination apparatus can be used in a microscope.
    Type: Application
    Filed: December 3, 2020
    Publication date: June 24, 2021
    Inventors: Jörg Siebenmorgen, Ralf Netz
  • Publication number: 20210132354
    Abstract: An optical arrangement for light beam shaping in a light microscope has a first and a second liquid crystal region, each of which has a plurality of independently switchable liquid crystal elements with which a phase of incident light is changeable in a settable manner. A first polarization beam splitter is arranged in such a way that incident light is split in a polarization-dependent manner into reflection light, which is reflected in the direction of the first liquid crystal region, and transmission light, which is transmitted in the direction of the second liquid crystal region. The first or a second polarization beam splitter is arranged such that the reflection light and transmission light are combined onto a common beam path after phase modulation by means of the liquid crystal regions.
    Type: Application
    Filed: April 11, 2019
    Publication date: May 6, 2021
    Applicant: Carl Zeiss Microscopy GmbH
    Inventors: Jörg SIEBENMORGEN, lngo KLEPPE, Ralf NETZ
  • Patent number: 10989909
    Abstract: A method for providing an overview image of an object that is arranged in a sample plane and includes generating a light sheet, capturing detection light coming from the sample plane, imaging the captured detection light by means of a detector in a detection plane in the form of image data of at least one captured image, wherein the captured image extends in an image plane that is inclined with respect to the sample plane, capturing a number of images of at least one region of the object, in the form of an inclined stack, and transforming the inclined stack to a normalized Z-stack, in which image data of the captured images are assigned with correct orientation with respect to the reference axis. A maximum intensity projection in the normalized Z-stack, wherein a resulting overview image is generated by way of selected image points being imaged as a virtual projection into a projection plane that is parallel to the image plane of the detector.
    Type: Grant
    Filed: May 16, 2019
    Date of Patent: April 27, 2021
    Assignee: Carl Zeiss Microscopy GmbH
    Inventor: Jörg Siebenmorgen
  • Patent number: 10983322
    Abstract: A single plane illumination microscope having an illumination optical system for illuminating a sample located on a sample carrier in a medium, and which is parallel to a planar reference surface. The sample is illuminated by a light sheet via an illumination light path. A detection optical system has a detection beam path. The optical axes of the illumination and detection optical systems each define an angle that is not equal to zero degrees along with the normal to the reference surface. A barrier layer system includes at least one layer of a given material having a given thickness and separates the medium from the illumination and detection optical systems. A base area of the barrier layer system is in contact with the region that is accessible for illumination and detection activities, said base area running parallel to the reference surface.
    Type: Grant
    Filed: October 20, 2017
    Date of Patent: April 20, 2021
    Assignee: Carl Zeiss Microscopy GmbH
    Inventors: Thomas Kalkbrenner, Ralf Netz, Helmut Lippert, Joerg Siebenmorgen
  • Publication number: 20210096358
    Abstract: An optical arrangement for light beam shaping for a light microscope has a first and a second liquid crystal region or lifting micromirror region, each of which has a plurality of independently switchable liquid crystal elements or mirrors with which a phase of incident light is changeable in a settable manner, an input-/output-coupling polarization beam splitter, a polarization beam splitter arranged between the input-/output-coupling polarization beam splitter and the liquid crystal regions or lifting micromirror regions such that the polarization beam splitter separates the light coming from the input-/output-coupling polarization beam splitter in a polarization-dependent manner into a first partial beam, which is directed to the first liquid crystal region or lifting micromirror region, and into a second partial beam, which is directed to the second liquid crystal region or lifting micromirror region, and that the polarization beam splitter combines the two partial beams returning from the liquid crystal
    Type: Application
    Filed: April 11, 2019
    Publication date: April 1, 2021
    Applicant: Cart Zeiss Microscopy GmbH
    Inventors: Dr. Jörg SIEBENMORGEN, Ingo KLEPPE, Ralf NETZ
  • Publication number: 20200371336
    Abstract: A method for operating a microscopy arrangement, and a microscopy arrangement, having a first microscope and at least one further microscope, wherein each of the microscopes have a respective optical axis. The respective optical axes do not coincide. The method provides a three-dimensional reference coordinate system being set; a carrier apparatus, that is embodied in the arrangement to receive and hold a specimen carrier is introduced into a specimen plane of the first microscope that is intersected by the optical axis and onto the optical axis of the first microscope; a reference point is set on the optical axis of the first microscope; the carrier apparatus is delivered to the further microscope, wherein the current coordinates of the reference point are continuously captured and compared to the coordinates of the optical axis of the at least one further microscope; and the reference point is brought onto the optical axis of the at least one further microscope.
    Type: Application
    Filed: August 11, 2020
    Publication date: November 26, 2020
    Applicant: Carl Zeiss Microscopy GmbH
    Inventors: Thomas KALKBRENNER, Saskia PERGANDE, Jörg SIEBENMORGEN, Helmut LIPPERT