Abstract: An electrical switching circuit for operating a bus of a field bus system senses a fault current based on a first current of a first bus line and a second current of a second bus line.

Abstract: The invention relates to an electric switching circuit for operating a bus of a field bus system comprising means for sensing a fault current. Said means comprise first means for determining a first current of a first bus line, second means for determining a second current of a second bus line and means for detecting a fault based on the first current and the second current.

Abstract: The invention relates to a method for marker-free DNA expression cassette exchange in the genome of cells or parts of cells by using the FLP recombinase mediated cassette exchange. A first DNA expression cassette carrying a positive-negative selection marker flanked by two FLP recombinase recognition target (FRT) sites is integrated into a chromosomal locus of the genome. Following selection of cell clones surviving the conditions for positive selection, the first DNA cassette is exchanged by an incoming second DNA expression cassette located on a circular vector and carrying a transgene flanked by the same FRT sites as the first DNA cassette by using FLP-recombinase. The cell clones surviving the conditions for negative selection contain specifically inserted the gene of the incoming DNA cassette without inserted unwanted vector sequences or positive selectable markers.

Abstract: The present invention relates to stably episomally replicating vectors, comprising at least one scaffold/matrix attached region (S/MAR) and at least one viral or eukaryotic origin of replication (ORI), cells comprising these, processes for their preparation, and their use, in particular as a medicament or diagnostic.