Patents by Inventor J. Tanner Nevill
J. Tanner Nevill has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20250084357Abstract: Systems, methods and kits are described for culturing one or more biological cells in a microfluidic device, including provision of nutrients and gaseous components configured to enhance cell growth, viability, portability, or any combination thereof. In some embodiments, culturing a single cell may produce a clonal population in the microfluidic device.Type: ApplicationFiled: September 23, 2024Publication date: March 13, 2025Inventors: Randall D. LOWE, JR., Kristin G. BEAUMONT, Aathavan KARUNAKARAN, Natalie C. MARKS, Jason M. MCEWEN, Mark P. WHITE, J. Tanner NEVILL, Gang F. WANG, Andrew W. MCFARLAND, Daniele MALLEO, Keith J. BREINLINGER, Xiao GUAN, Kevin T. CHAPMAN
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Publication number: 20250027941Abstract: This application describes systems and methods for assaying micro-objects in a microfluidic device. These methods include contacting a reagent with a micro-object by introducing a reagent in a first fluidic medium to a flow region of a microfluidic device, wherein the microfluidic device comprises the flow region and a chamber comprising a proximal opening fluidically connecting the chamber to the flow region, diffusing the reagent from the flow region into the chamber; introducing a micro-object into the flow region of the microfluidic device, and diffusing the reagent from the chamber to the flow region to contact the reagent with the micro-object within the flow region. Other embodiments are described.Type: ApplicationFiled: July 25, 2024Publication date: January 23, 2025Applicant: BRUKER CELLULAR ANALYSIS, INC.Inventors: Ke-Chih LIN, Long Van LE, Jason M. MCEWEN, J. Tanner NEVILL, Volker L.S. KURZ, Peyton SHIEH, Alexander J. MASTROIANNI, Or GADISH, Ethan Jun Wei GOH
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Patent number: 12138626Abstract: In situ-generated microfluidic isolation structures incorporating a solidified polymer network, methods of preparation and use, compositions and kits therefor are described. The ability to introduce in real time, a variety of isolating structures including pens and barriers offers improved methods of micro-object manipulation in microfluidic devices. The in situ-generated isolation structures may be permanently or temporarily installed.Type: GrantFiled: February 3, 2023Date of Patent: November 12, 2024Assignee: BRUKER CELLULAR ANALYSIS, INC.Inventors: Kristin G. Beaumont, Nan-Linda Ding, Volker L. S. Kurz, Troy A. Lionberger, Randall D. Lowe, Jr., Daniele Malleo, Andrew W. McFarland, J. Tanner Nevill, Xiaohua Wang
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Patent number: 12134758Abstract: Systems, methods and kits are described for culturing one or more biological cells in a microfluidic device, including provision of nutrients and gaseous components configured to enhance cell growth, viability, portability, or any combination thereof. In some embodiments, culturing a single cell may produce a clonal population in the microfluidic device.Type: GrantFiled: May 12, 2022Date of Patent: November 5, 2024Assignee: BRUKER CELLULAR ANALYSIS, INC.Inventors: Randall D. Lowe, Jr., Kristin G. Beaumont, Aathavan Karunakaran, Natalie C. Marks, Jason M. McEwen, Mark P. White, J. Tanner Nevill, Gang F. Wang, Andrew W. McFarland, Daniele Malleo, Keith J. Breinlinger, Xiao Guan, Kevin T. Chapman
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Patent number: 11998914Abstract: Biological activity in holding pens in a micro-fluidic device can be assayed by placing in the holding pens capture objects that bind a particular material of interest produced by the biological activity. The biological material of interest that binds to each capture object can then be assessed, either in the micro-fluidic device or after exporting the capture object from the micro-fluidic device. The assessment can be utilized to characterize the biological activity in each holding pen. The biological activity can be production of the biological material of interest. Thus, the biological activity can correspond to or arise from one or more biological cells. Biological cells within a holding pen can be clonal cell colonies. The biological activity of each clonal cell colony can be assayed while maintaining the clonal status of each colony.Type: GrantFiled: March 24, 2022Date of Patent: June 4, 2024Assignee: BRUKER CELLULAR ANALYSIS, INC.Inventors: Kevin T. Chapman, Daniele Malleo, J. Tanner Nevill, Steven W. Short, Mark P. White, M. Jimena Loureiro
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Publication number: 20240027396Abstract: Individual biological micro-objects can be deterministically selected and moved into holding pens in a micro-fluidic device. A flow of a first liquid medium can be provided to the pens. Physical pens can be structured to impede a direct flow of the first medium into a second medium in the pens while allowing diffusive mixing of the first medium and the second medium. Virtual pens can allow a common flow of medium to multiple ones of the pens.Type: ApplicationFiled: May 25, 2023Publication date: January 25, 2024Inventors: Kevin T. Chapman, Igor Y. Khandros, Gaetan L. Mathieu, J. Tanner Nevill, Ming C. Wu
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Publication number: 20230384573Abstract: A system for operating an electrokinetic device includes a support configured to hold and operatively couple with the electrokinetic device, an integrated electrical signal generation subsystem configured to apply a biasing voltage across a pair of electrodes in the electrokinetic device, and a light modulating subsystem configured to emit structured light onto the electrokinetic device. The system can further include a thermally controlled flow controller, and/or be configured to measure impedance across the electrokinetic device. The system can be a light microscope, including an optical train. The system can further include a light pipe, which can be part of the light modulating system, and which can be configured to supply light of substantially uniform intensity to the light modulating system or directly to the optical train.Type: ApplicationFiled: August 10, 2023Publication date: November 30, 2023Inventors: Andrew W. McFarland, Daniele Malleo, J. Tanner Nevill, Russell A. Newstrom, Keith J. Breinlinger, Paul M. Lundquist, Justin K. Valley, Jonathan Cloud Dragon Hubbard
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MICROFLUIDIC DEVICES HAVING ISOLATION PENS AND METHODS OF TESTING BIOLOGICAL MICRO-OBJECTS WITH SAME
Publication number: 20230347347Abstract: A microfluidic device can comprise at least one swept region that is fluidically connected to unswept regions. The fluidic connections between the swept region and the unswept regions can enable diffusion but substantially no flow of media between the swept region and the unswept regions. The capability of biological micro-objects to produce an analyte of interest can be assayed in such a microfluidic device. Biological micro-objects in sample material loaded into a microfluidic device can be selected for particular characteristics and disposed into unswept regions. The sample material can then be flowed out of the swept region and an assay material flowed into the swept region. Flows of medium in the swept region do not substantially affect the biological micro-objects in the unswept regions, but any analyte of interest produced by a biological micro-object can diffuse from an unswept region into the swept region, where the analyte can react with the assay material to produce a localized detectable reaction.Type: ApplicationFiled: December 19, 2022Publication date: November 2, 2023Inventors: Mark P. White, Eric D. Hobbs, J. Tanner Nevill, Daniele Malleo, Steven W. Short -
Patent number: 11802264Abstract: Apparatuses and methods are described for the use of optically driven bubble, convective and displacing fluidic flow to provide motive force in microfluidic devices. Alternative motive modalities are useful to selectively dislodge and displace micro-objects, including biological cells, from a variety of locations within the enclosure of a microfluidic device.Type: GrantFiled: October 12, 2020Date of Patent: October 31, 2023Assignee: PHENOMEX INC.Inventors: Volker L. S. Kurz, Troy A. Lionberger, Eric K. Sackmann, Kai W. Szeto, Paul M. Lebel, Brandon R. Bruhn, Keith J. Breinlinger, Eric D. Hobbs, Andrew W. McFarland, J. Tanner Nevill, Xiaohua Wang
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Publication number: 20230242860Abstract: Incubators including an enclosure with an internal chamber configured to support a cell culture plate comprising a plurality of wells are disclosed. The enclosure includes a plurality of openings configured to allow access to the wells. The incubators include a sealing element configured to seal the plurality of openings in the enclosure. The sealing element comprises a plurality of openings corresponding to at least a subset of the plurality of openings in the enclosure. Access to the internal chamber can be provided by aligning the plurality of openings in the sealing element with the plurality of openings in the enclosure. Methods for using the incubators are also provided.Type: ApplicationFiled: September 29, 2022Publication date: August 3, 2023Inventors: Russell A. NEWSTROM, Andrew W. MCFARLAND, Darcy K. KELLY-GREENE, J. Tanner NEVILL, Gang F. WANG
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Publication number: 20230182136Abstract: In situ-generated microfluidic isolation structures incorporating a solidified polymer network, methods of preparation and use, compositions and kits therefor are described. The ability to introduce in real time, a variety of isolating structures including pens and barriers offers improved methods of micro-object manipulation in microfluidic devices. The in situ-generated isolation structures may be permanently or temporarily installed.Type: ApplicationFiled: February 3, 2023Publication date: June 15, 2023Applicant: BERKELEY LIGHTS, INC.Inventors: Kristin G. Beaumont, Non-Linda Ding, Volker L.S. Kurz, Troy A. Lionberger, Randall D. Lowe, JR., Daniele Malleo, Andrew W. McFarland, J. Tanner Nevill, Xiaohua Wang
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Patent number: 11666913Abstract: In situ-generated microfluidic isolation structures incorporating a solidified polymer network, methods of preparation and use, compositions and kits therefor are described. The ability to introduce in real time, a variety of isolating structures including pens and barriers offers improved methods of micro-object manipulation in microfluidic devices. The in situ-generated isolation structures may be permanently or temporarily installed.Type: GrantFiled: November 22, 2016Date of Patent: June 6, 2023Assignee: BERKELEY LIGHTS, INCInventors: Kristin G. Beaumont, Nan-Linda Ding, Volker L. S. Kurz, Troy A. Lionberger, Randall D. Lowe, Jr., Daniele Malleo, Andrew W. McFarland, J. Tanner Nevill, Xiaohua Wang
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Microfluidic devices having isolation pens and methods of testing biological micro-objects with same
Patent number: 11565259Abstract: A microfluidic device can comprise at least one swept region that is fluidically connected to unswept regions. The fluidic connections between the swept region and the unswept regions can enable diffusion but substantially no flow of media between the swept region and the unswept regions. The capability of biological micro-objects to produce an analyte of interest can be assayed in such a microfluidic device. Biological micro-objects in sample material loaded into a microfluidic device can be selected for particular characteristics and disposed into unswept regions. The sample material can then be flowed out of the swept region and an assay material flowed into the swept region. Flows of medium in the swept region do not substantially affect the biological micro-objects in the unswept regions, but any analyte of interest produced by a biological micro-object can diffuse from an unswept region into the swept region, where the analyte can react with the assay material to produce a localized detectable reaction.Type: GrantFiled: November 14, 2019Date of Patent: January 31, 2023Assignee: Berkeley Lights, Inc.Inventors: Mark P. White, Eric D. Hobbs, J. Tanner Nevill, Daniele Malleo, Steven W. Short -
Publication number: 20220388003Abstract: Biological activity in holding pens in a micro-fluidic device can be assayed by placing in the holding pens capture objects that bind a particular material of interest produced by the biological activity. The biological material of interest that binds to each capture object can then be assessed, either in the micro-fluidic device or after exporting the capture object from the micro-fluidic device. The assessment can be utilized to characterize the biological activity in each holding pen. The biological activity can be production of the biological material of interest. Thus, the biological activity can correspond to or arise from one or more biological cells. Biological cells within a holding pen can be clonal cell colonies. The biological activity of each clonal cell colony can be assayed while maintaining the clonal status of each colony.Type: ApplicationFiled: March 24, 2022Publication date: December 8, 2022Inventors: Kevin T. Chapman, Daniele Malleo, J. Tanner Nevill, Steven W. Short, Mark P. White, M. Jimena Loureiro
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Publication number: 20220356429Abstract: Systems, methods and kits are described for culturing one or more biological cells in a microfluidic device, including provision of nutrients and gaseous components configured to enhance cell growth, viability, portability, or any combination thereof. In some embodiments, culturing a single cell may produce a clonal population in the microfluidic device.Type: ApplicationFiled: May 12, 2022Publication date: November 10, 2022Inventors: Randall D. LOWE, JR., Kristin G. BEAUMONT, Aathavan KARUNAKARAN, Natalie C. MARKS, Jason M. MCEWEN, Mark P. WHITE, J. Tanner NEVILL, Gang F. WANG, Andrew W. MCFARLAND, Daniele Malleo, Keith J. BREINLINGER, Xiao GUAN, Kevin T. CHAPMAN
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Patent number: 11492584Abstract: Incubators including an enclosure with an internal chamber configured to support a cell culture plate comprising a plurality of wells are disclosed. The enclosure includes a plurality of openings configured to allow access to the wells. The incubators include a sealing element configured to seal the plurality of openings in the enclosure. The sealing element comprises a plurality of openings corresponding to at least a subset of the plurality of openings in the enclosure. Access to the internal chamber can be provided by aligning the plurality of openings in the sealing element with the plurality of openings in the enclosure. Methods for using the incubators are also provided.Type: GrantFiled: August 9, 2019Date of Patent: November 8, 2022Assignee: Berkeley Lights, Inc.Inventors: Russell A. Newstrom, Andrew W. McFarland, Darcy K. Kelly-Greene, J. Tanner Nevill, Gang F. Wang
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Patent number: 11434462Abstract: Incubators are disclosed which include an enclosure with an internal chamber configured to support a cell culture plate and provide an environment suitable for maintaining and/or culturing biological cells. The enclosure can include one or more openings configured to allow access to the cell culture plate. The incubators can further include a structure having a plurality of openings configured to be aligned with a corresponding plurality of wells in the cell culture plate, and a sealing element configured to moveably seal the plurality of openings in the structure. The sealing element can comprise a plurality of openings corresponding to at least a subset of the plurality of openings of the structure. Access to the internal chamber can be provided by aligning the plurality of openings in the sealing element with the plurality of openings in the structure. Methods for using the incubators are also provided.Type: GrantFiled: May 31, 2019Date of Patent: September 6, 2022Assignee: Berkeley Lights, Inc.Inventors: Darcy K. Kelly-Greene, Russell A. Newstrom, Andrew W. McFarland, J. Tanner Nevill, Gang F. Wang
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Publication number: 20220250071Abstract: Methods, systems and kits are described herein for detecting the results of an assay. In particular, the methods, systems and devices of the present disclosure rely on a difference between the diffusion rates of a reporter molecule and an analyte of interest in order to quantify an amount of analyte in a microfluidic device. The analyte may be a secreted product of a biological micro-object.Type: ApplicationFiled: November 23, 2021Publication date: August 11, 2022Inventors: Troy A. LIONBERGER, Matthew E. FOWLER, Phillip J. M. ELMS, Kevin D. LOUTHERBACK, Randall D. LOWE, JR., Jian GONG, J. Tanner NEVILL, Gang F. WANG, Gregory G. LAVIEU, John A. TENNEY, Aathavan KARUNAKARAN, Anupam SINGHAL, I-Jong LIN
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Patent number: 11365381Abstract: Systems, methods and kits are described for culturing one or more biological cells in a microfluidic device, including provision of nutrients and gaseous components configured to enhance cell growth, viability, portability, or any combination thereof. In some embodiments, culturing a single cell may produce a clonal population in the microfluidic device.Type: GrantFiled: July 2, 2020Date of Patent: June 21, 2022Assignee: Berkeley Lights, Inc.Inventors: Randall D. Lowe, Jr., Kristin G. Beaumont, Aathavan Karunakaran, Natalie C. Marks, Jason M. McEwen, Mark P. White, J. Tanner Nevill, Gang F. Wang, Andrew W. McFarland, Daniele Malleo, Keith J. Breinlinger, Xiao Guan, Kevin T. Chapman
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Publication number: 20220143612Abstract: A microfluidic apparatus is provided having one or more sequestration pens configured to isolate one or more target micro-objects by changing the orientation of the microfluidic apparatus with respect to a globally active force, such as gravity. Methods of selectively directing the movements of micro-objects in such a microfluidic apparatus using gravitational forces are also provided. The micro-objects can be biological micro-objects, such as cells, or inanimate micro-objects, such as beads.Type: ApplicationFiled: October 30, 2020Publication date: May 12, 2022Inventors: Keith J. Breinlinger, Eric D. Hobbs, Daniele Malleo, J. Tanner Nevill, Mark P. White