Patents by Inventor J. William Efcavitch
J. William Efcavitch has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
-
Publication number: 20130022977Abstract: The invention generally relates to methods for detecting fetal nucleic acids and methods for diagnosing fetal abnormalities. In certain embodiments, the invention provides methods for determining whether fetal nucleic acid is present in a maternal sample including obtaining a maternal sample suspected to include fetal nucleic acids, and performing a sequencing reaction on the sample to determine presence of at least a portion of a Y chromosome in the sample, thereby determining that fetal nucleic acid is present in the sample. In other embodiments, the invention provides methods for quantitative or qualitative analysis to detect fetal nucleic acid in a maternal sample, regardless of the ability to detect the Y chromosome, particularly for samples including normal nucleic acids from a female fetus.Type: ApplicationFiled: September 14, 2012Publication date: January 24, 2013Applicant: SEQUENOM, INCInventors: Stanley Lapidus, John F. Thompson, Doron Lipson, Patrice Milos, J. William Efcavitch, Stanley Letovsky
-
Publication number: 20120040340Abstract: The invention provides for nucleotide analogs and methods of using the same, e.g., for sequencing nucleic acids.Type: ApplicationFiled: October 27, 2011Publication date: February 16, 2012Applicant: HELICOS BIOSCIENCES CORPORATIONInventors: J. William Efcavitch, Suhaib Siddiqi, Philip R. Buzby, Judith Mitchell, Edyta Krzymanska-Olejnik, Subramanian Marappan, Xiaopeng Bai, Atanu Roy, Mirna Jarosz, Jayson Bowers
-
Patent number: 8071755Abstract: The invention provides for nucleotide analogs and methods of using the same, e.g., for sequencing nucleic acids.Type: GrantFiled: April 4, 2008Date of Patent: December 6, 2011Assignee: Helicos Biosciences CorporationInventors: J. William Efcavitch, Suhaib Siddiqi, Philip R. Buzby, Judith Mitchell, Edyta Krzymanska-Olejnik, Subramanian Marappan, Xiaopeng Bai, Atanu Roy, Mirna Jarosz, Jayson Bowers
-
Publication number: 20110192725Abstract: The invention provides uncharged water-soluble silica-adsorbing polymers for suppressing electroendoosmotic flow and to reduce analyte-wall interactions in capillary electrophoresis. In one aspect of the invention, one or more of such polymers are employed as components of a separation medium for the separation of biomolecules, such as polynucleotides, polysaccharides, proteins, and the like, by capillary electrophoresis. Generally, such polymers are characterized by (i) water solubility over the temperature range between about 20° C. to about 50° C., (ii) concentration in a separation medium in the range between about 0.001% to about 10% (weight/volume), (iii) molecular weight in the range of about 5×103 to about 1×106 daltons, and (iv) absence of charged groups in an aqueous medium having pH in the range of about 6 to about 9.Type: ApplicationFiled: December 23, 2010Publication date: August 11, 2011Applicant: LIFE TECHNOLOGIES CORPORATIONInventors: Ramakrishna S. Madabhushi, Steven M. Menchen, J. William Efcavitch, Paul D. Grossman
-
Publication number: 20110081647Abstract: The invention generally relates to nucleotide analogs and methods of their use in sequencing-by-synthesis reactions. In certain embodiments, the invention provides a nucleotide analog including a detectable label attached to a nitrogenous base portion of a nucleotide analog by a cleavable linker, in which contact of the analog with at least one activating agent results in cleavage of the label and elimination of the linker, thereby producing a natural nucleotide, a 9-deaza-G, 9-deaza-A, or ?-uridine.Type: ApplicationFiled: December 4, 2009Publication date: April 7, 2011Applicant: HELICOS BIOSCIENCES CORPORATIONInventors: Suhaib Siddiqi, J. William Efcavitch, Judith Mitchell, Subramanian Marappan, Jayson Bowers, Mirna Jaroza
-
Patent number: 7863357Abstract: The invention provides uncharged water-soluble silica-adsorbing polymers for suppressing electroendoosmotic flow and to reduce analyte-wall interactions in capillary electrophoresis. In one aspect of the invention, one or more of such polymers are employed as components of a separation medium for the separation of biomolecules, such as polynucleotides, polysaccharides, proteins, and the like, by capillary electrophoresis. Generally, such polymers are characterized by (i) water solubility over the temperature range between about 20° C. to about 50° C., (ii) concentration in a separation medium in the range between about 0.001% to about 10% (weight/volume), (iii) molecular weight in the range of about 5×103 to about 1×106 daltons, and (iv) absence of charged groups in an aqueous medium having pH in the range of about 6 to about 9.Type: GrantFiled: February 21, 2006Date of Patent: January 4, 2011Assignee: Life Technologies CorporationInventors: Ramakrishna S. Madabhushi, Steven M. Menchen, J. William Efcavitch, Paul D. Grossman
-
Publication number: 20100233696Abstract: A method, flow cell and/or device for increasing the recovery of a limiting analyte in a sample, e.g., for single molecule analysis is disclosed. Methods for preparing a nucleic acid sample from a single cell and capturing nucleic acids on a surface configured for use in or with single molecule analysis are also provided.Type: ApplicationFiled: November 4, 2009Publication date: September 16, 2010Applicant: HELICOS BIOSCIENCES CORPORATIONInventors: Richard Joseph, James J. DiMeo, Mirna Jarosz, John F. Thompson, Jayson Bowers, Scott Chouinard, Philipp Kapronov, J. William Efcavitch, Christopher Hart, Fatih Ozsolak
-
Publication number: 20100216153Abstract: The invention generally relates to methods for detecting fetal nucleic acids and methods for diagnosing fetal abnormalities. In certain embodiments, the invention provides methods for determining whether fetal nucleic acid is present in a maternal sample including obtaining a maternal sample suspected to include fetal nucleic acids, and performing a sequencing reaction on the sample to determine presence of at least a portion of a Y chromosome in the sample, thereby determining that fetal nucleic acid is present in the sample. In other embodiments, the invention provides methods for quantitative or qualitative analysis to detect fetal nucleic acid in a maternal sample, regardless of the ability to detect the Y chromosome, particularly for samples including normal nucleic acids from a female fetus.Type: ApplicationFiled: March 19, 2010Publication date: August 26, 2010Applicant: HELICOS BIOSCIENCES CORPORATIONInventors: STANLEY LAPIDUS, JOHN F. THOMPSON, DORON LIPSON, PATRICE MILOS, J. WILLIAM EFCAVITCH, STANLEY LETOVSKY
-
Publication number: 20100216151Abstract: The invention generally relates to methods for detecting fetal nucleic acids and methods for diagnosing fetal abnormalities. In certain embodiments, the invention provides methods for determining whether fetal nucleic acid is present in a maternal sample including obtaining a maternal sample suspected to include fetal nucleic acids, and performing a sequencing reaction on the sample to determine presence of at least a portion of a Y chromosome in the sample, thereby determining that fetal nucleic acid is present in the sample. In other embodiments, the invention provides methods for quantitative or qualitative analysis to detect fetal nucleic acid in a maternal sample, regardless of the ability to detect the Y chromosome, particularly for samples including normal nucleic acids from a female fetus.Type: ApplicationFiled: February 19, 2010Publication date: August 26, 2010Applicant: Helicos BioSciences CorporationInventors: STANLEY LAPIDUS, JOHN F. THOMPSON, DORON LIPSON, PATRICE MILOS, J. WILLIAM EFCAVITCH, STANLEY LETOVSKY
-
Publication number: 20100203524Abstract: The invention generally relates to polymerases for efficient and controlled sequencing-by-synthesis reactions. In certain embodiments, the invention provides a polymerase enzyme including at least one mutation that enhances ability of the polymerase as compared to a wild-type polymerase to incorporate a nucleotide into a nascent strand of DNA or cDNA including at least one modified nucleotide.Type: ApplicationFiled: October 30, 2009Publication date: August 12, 2010Applicant: Helicos Biosciences CorporationInventors: J. William Efcavitch, Jayson L. Bowers, Philip R. Buzby, John F. Thompson
-
Publication number: 20090061437Abstract: The invention provides for nucleotide analogs and methods of using the same, e.g., for sequencing nucleic acids.Type: ApplicationFiled: April 4, 2008Publication date: March 5, 2009Applicant: HELICOS BIOSCIENCES CORPORATIONInventors: J. William Efcavitch, Suhaib Siddiqi, Philip R. Buzby, Judith Mitchell, Edyta Krzymanska-Olejnik, Subramanian Marappan, Xiaopeng Bai, Atanu Roy, Mirna Jarosz, Jayson Bowers
-
Publication number: 20070196832Abstract: The invention relates to methods for detecting a mutation in a nucleic acid. Methods of the invention are useful for detecting and identifying mutations that are indicative of disease or the predisposition for disease.Type: ApplicationFiled: February 22, 2006Publication date: August 23, 2007Inventors: J. William Efcavitch, Marie Sutherlin Causey
-
Patent number: 7045048Abstract: The invention provides uncharged water-soluble silica-adsorbing polymers for suppressing electroendoosmotic flow and to reduce analyte-wall interactions in capillary electrophoresis. In one aspect of the invention, one or more of such polymers are employed as components of a separation medium for the separation of biomolecules, such as polynucleotides, polysaccharides, proteins, and the like, by capillary electrophoresis. Generally, such polymers are characterized by (i) water solubility over the temperature range between about 20° C. to about 50° C., (ii) concentration in a separation medium in the range between about 0.001% to about 10% (weight/volume), (iii) molecular weight in the range of about 5×103 to about 1×106 daltons, and (iv) absence of charged groups in an aqueous medium having pH in the range of about 6 to about 9.Type: GrantFiled: December 28, 2001Date of Patent: May 16, 2006Assignee: Applera CorporationInventors: Ramakrishna S. Madabhushi, Steven M. Menchen, J. William Efcavitch, Paul D. Grossman
-
Patent number: 6827830Abstract: An electrophoretic method for purifying a nucleic acid sample is disclosed. According to the method, the electrophoresis is effective to substantially reduce the concentration of contaminants relative to the concentration of desired nucleic acid in the nucleic acid sample, thereby producing a purified nucleic acid. In the method, the loading and recovery wells may be the same or different, and the electric fields may be DC or alternating.Type: GrantFiled: September 20, 2000Date of Patent: December 7, 2004Assignees: Applera Corporation, University of OttawaInventors: Gary Slater, J. William Efcavitch, Guy Drouin, Pascal Mayer, Jean Rousseau, Hong Yan Zhou, Claudia Chiesa, Robert Ruhfel, Roger O'Neill
-
Publication number: 20030217924Abstract: The invention provides uncharged water-soluble silica-adsorbing polymers for suppressing electroendoosmotic flow and to reduce analyte-wall interactions in capillary electrophoresis. In one aspect of the invention, one or more of such polymers are employed as components of a separation medium for the separation of biomolecules, such as polynucleotides, polysaccharides, proteins, and the like, by capillary electrophoresis. Generally, such polymers are characterized by (i) water solubility over the temperature range between about 20° C. to about 50° C., (ii) concentration in a separation medium in the range between about 0.001% to about 10% (weight/volume), (iii) molecular weight in the range of about 5×103 to about 1×106 daltons, and (iv) absence of charged groups in an aqueous medium having pH in the range of about 6 to about 9.Type: ApplicationFiled: December 28, 2001Publication date: November 27, 2003Applicant: PE Corporation (NY)Inventors: Ramakrishna S. Madabhushi, Steven M. Menchen, J. William Efcavitch, Paul D. Grossman
-
Patent number: 6358385Abstract: The invention provides uncharged water-soluble silica-adsorbing polymers for suppressing electroendoosmotic flow and to reduce analyte-wall interactions in capillary electrophoresis. In one aspect of the invention, one or more of such polymers are employed as components of a separation medium for the separation of biomolecules, such as polynucleotides, polysaccharides, proteins, and the like, by capillary electrophoresis. Generally, such polymers are characterized by (i) water solubility over the temperature range between about 20° C. to about 50° C., (ii) concentration in a separation medium in the range between about 0.001% to about 10% (weight/volume), (iii) molecular weight in the range of about 5×103 to about 1×106 daltons, and (iv) absence of charged groups in an aqueous medium having pH in the range of about 6 to about 9.Type: GrantFiled: January 11, 1999Date of Patent: March 19, 2002Assignee: The Perkin-Elmer CorporationInventors: Ramakrishna S. Madabhushi, Steven M. Menchen, J. William Efcavitch, Paul D. Grossman
-
Patent number: 6355709Abstract: The invention provides uncharged water-soluble silica-adsorbing polymers for suppressing electroendoosmotic flow and to reduce analyte-wall interactions in capillary electrophoresis. In one aspect of the invention, one or more of such polymers are employed as components of a separation medium for the separation of biomolecules, such as polynucleotides, polysaccharides, proteins, and the like, by capillary electrophoresis. Generally, such polymers are characterized by (i) water solubility over the temperature range between about 20° C. to about 50° C., (ii) concentration in a separation medium in the range between about 0.001% to about 10% (weight/volume), (iii) molecular weight in the range of about 5×103 to about 1×106 daltons, and (iv) absence of charged groups in an aqueous medium having pH in the range of about 6 to about 9.Type: GrantFiled: October 15, 1997Date of Patent: March 12, 2002Assignee: The Perkin-Elmer CorporationInventors: Ramakrishna S. Madabhushi, Steven M. Menchen, J. William Efcavitch, Paul D. Grossman
-
Patent number: 6146511Abstract: An electrophoretic method for purifying a nucleic acid sample is disclosed. The method generally comprises the steps of (1) providing a nucleic acid sample comprising a desired nucleic acid and one or more contaminants, (2) providing an electrophoresis matrix having a loading well and a recovery well formed therein, (3) placing the nucleic acid sample into the loading well, (4) performing a first electrophoresis comprising electrophoresing the nucleic acid sample for a first time effective to transport the desired nucleic acid out of the loading well and into the electrophoresis matrix; and (5) performing a second electrophoresis comprising electrophoresing the nucleic acid sample for a second time effective to transport the desired nucleic acid out of the electrophoresis matrix and into the recovery well.Type: GrantFiled: January 30, 1998Date of Patent: November 14, 2000Assignees: The Perkin-Elmer Corporation, The University of OttawaInventors: Gary Slater, J. William Efcavitch, Guy Drouin, Pascal Mayer, Jean Rousseau, Hong Yan Zhou, Claudia Chiesa, Robert Ruhfel, Roger O'Neill
-
Patent number: 5916426Abstract: The invention provides uncharged water-soluble silica-adsorbing polymers for suppressing electroendoosmotic flow and to reduce analyte-wall interactions in capillary electrophoresis. In one aspect of the invention, one or more of such polymers are employed as components of a separation medium for the separation of biomolecules, such as polynucleotides, polysaccharides, proteins, and the like, by capillary electrophoresis. Generally, such polymers are characterized by (i) water solubility over the temperature range between about 20.degree. C. to about 50.degree. C., (ii) concentration in a separation medium in the range between about 0.001% to about 10% (weight/volume), (iii) molecular weight in the range of about 5.times.10.sup.3 to about 1.times.10.sup.6 daltons, and (iv) absence of charged groups in an aqueous medium having pH in the range of about 6 to about 9.Type: GrantFiled: August 19, 1997Date of Patent: June 29, 1999Assignee: The Perkin-Elmer CorporationInventors: Ramakrishna S. Madabhushi, Steven M. Menchen, J. William Efcavitch, Paul D. Grossman
-
Patent number: 5567292Abstract: The invention provides uncharged water-soluble silica-adsorbing polymers for suppressing electroendoosmotic flow and to reduce analyte-wall interactions in capillary electrophoresis. In one aspect of the invention, one or more of such polymers are employed as components of a separation medium for the separation of biomolecules, such as polynucleotides, polysaccharides, proteins, and the like, by capillary electrophoresis. Generally, such polymers are characterized by (i) water solubility over the temperature range between about 20.degree. C. to about 50.degree. C., (ii) concentration in a separation medium in the range between about 0.001% to about 10% (weight/volume), (iii) molecular weight in the range of about 5.times.10.sup.3 to about 1.times.10.sup.6 daltons, and (iv) absence of charged groups in an aqueous medium having pH in the range of about 6 to about 9.Type: GrantFiled: December 6, 1994Date of Patent: October 22, 1996Assignee: The Perkin-Elmer CorporationInventors: Ramakrishna S. Madabhushi, Steven M. Menchen, J. William Efcavitch, Paul D. Grossman