Abstract: A method of detecting an enzyme-mediated DNA cleavage reaction in a fluorometric assay is provided. The method can be used to detect DNA cleavage caused by restriction endonucleases, retroviral integrase enzymes, DNases, RNases, or enzymes utilized in other strand separating processes in molecular biology.

Abstract: A method of detecting an enzyme-mediated DNA cleavage reaction in a fluorometric assay is provided. The method can be used to detect DNA cleavage caused by restriction endonucleases, retroviral integrase enzymes, DNases, RNases, or enzymes utilized in other strand separating processes in molecular biology.

Abstract: A method of detecting an enzyme-mediated DNA cleavage reaction in a fluorometric assay is provided. The method can be used to detect DNA cleavage caused by restriction endonucleases, retroviral integrase enzymes, DNases, RNases, or enzymes utilized in other strand separating processes in molecular biology.

Abstract: According to the present invention there is provided a method for detecting the presence of at least two point mutations in a target polynucleotide, as well as their relative positions and specific nucleotide positions, involving partial digestion and the use of mismatch repair enzymes.

Abstract: A method of detecting an enzyme-mediated DNA cleavage reaction in a fluorometric assay is provided. The method can be used to detect DNA cleavage caused by restriction endonucleases, retroviral integrase enzymes, DNases, RNases, or enzymes utilized in other strand separating processes in molecular biology.

Abstract: The present invention provides several methods employing nucleic acid repair enzymes. The present invention provides a method for detecting point mutations in nucleic acid sequences. The present invention further provides a method for detecting non-mutated or wild-type nucleic acid sequences. The present invention also enhances target polynucleotide detection using an oscillation reaction and tail labeling techniques, as well as by linking the nucleic acid repair enzyme to a probe molecule. The present invention also provides helix destabilizing molecule and similar molecules to enhance the hybridization of the probe to the target polynucleotides. This invention further provides for the use of thermally stable nucleic acid repair enzymes which will facilitate reactions at elevated temperatures. This invention also provides a method for determining the repair index for a mismatched or damaged oligonucleotide probe.

Abstract: The present invention provides several methods employing nucleic acid repair enzymes. The present invention provides a method for detecting point mutations in nucleic acid sequences. The present invention further provides a method for detecting non-mutated or wild-type nucleic acid sequences. The present invention also enhances target polynucleotide detection using an oscillation reaction and tail labeling techniques. The present invention also provides helix destabilizing molecule and similar molecules to enhance the hybridization of the probe to the target polynucleotides. Finally, this invention provides a method for determining the repair index for a mismatched or damaged oligonucleotide probe.

Abstract: The present invention relates to a method for cloning genes that encode restriction endonucleases by altering the level of a methyl donor co-factor of a DNA methyltransferase that protects the DNA of a host cell from damage by a restriction endonuclease. The method can be used to screen entire DNA libraries en masse to identify clones that encode restriction enzymes by growing one library replicate under high or normal methyl donor conditions to protect host DNA and a second library replicate under low methyl donor conditions allowing DNA damage from the active restriction endonuclease. Clones that encode a restriction enzyme are identified by decreased growth or color produced in response to double stranded DNA damage under the low methyl donor conditions. Colorimetric methods useful in the invention can use SOS-sensitive promoters operably linked to .beta.-galactosidase, which detect DNA damage.

Abstract: A room temperature stable restriction enzyme in a glassified carbohydrate stabilizer is disclosed. A restriction enzyme reaction buffer containing Mg.sup.+2 is dispersed in the stabilizer. One cleaves DNA merely by adding water and DNA to the composition. The composition can reduce glycerol-caused star activity by avoiding the need for glycerol. The composition also reduces star activity caused by high concentrations of enzymes. Another method is provided of comparing cleaved DNA samples in connection with forensic and paternity testing applications.

Abstract: A method for diagnosing mammalian breast cancer by detecting in the physiological fluid of said mammal an antigen (ACRT) having immune cross-reactivity with human reverse transcriptase, or detecting antibodies against said ACRT or detecting antibody-ACRT complexes, wherein the reverse transcriptase is substantially purified, has a molecular of about 70,000 and a sedimentation coefficient on a glycerol gradient of between 5 and 5.5 S. A process for the purification of reverse transcriptase from human milk.

Abstract: Restriction endonucleases are immobilized by chemically bonding the restriction endonucleases to water-insoluble matrices. The immobilized restriction endonucleases have enhanced stability and can be used to obtain deoxyribonucleic acid fragments in relatively low salt media, free of protein and ready for further use without tedious and time-consuming manipulations.

Abstract: The disclosure is of fluorescent deoxyribonucleic acid intercalating agent derivatives of activated polysaccharides such as cyanogen halide-activated agarose and the like. The derivatives are useful fluorescent stain ingredients of water-insoluble gels used to fractionate deoxyribonucleic acids and deoxyribonucleic acid fragments by gel electrophoresis techniques. As ingredients of electrophoretic grade gels, the derivatives of the invention function as fluorescent stains to provide direct visualization of the deoxyribonucleic acids and their fractions, under the excitation of shortwave, ultraviolet radiation.