Abstract: The present invention provides engineered ketoreductase and phosphite dehydrogenase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase and phosphite dehydrogenase enzymes, as well as polynucleotides encoding the engineered ketoreductase and phosphite dehydrogenase enzymes, host cells capable of expressing the engineered ketoreductase and phosphite dehydrogenase enzymes, and methods of using the engineered ketoreductase and phosphite dehydrogenase enzymes to synthesize a chiral catalyst used in the synthesis of antiviral compounds, such as nucleoside inhibitors. The present invention further provides methods of using the engineered enzymes to deracemize a chiral alcohol in a one-pot, multi-enzyme system.

Abstract: An automated tread registration system includes a sensing system configured to provide at least one surface model of a first object. The surface model of the first object has at least one pattern with at least one groove and at least one protrusion. The automated tread registration system also includes a user system executing processing software reading data corresponding to the at least one surface model of the first object. The surface model corresponds to a current condition of the first object. The processing software executed by the user system may analyze the at least one surface model of the first object and provide at least one indicative metric based on the analysis of the surface model. The indicative metric may be camber wear, toe wear, heel toe wear, overinflation, underinflation, and/or the like.

Abstract: Disclosed is a system and method for documenting and analyzing a tire tread using a guidance dolly for use in creating a three-dimensional image file of a target object from a plurality of two-dimensional image files including: a first dolly surface adapted to enable placement of the guidance dolly into physical contact with the target object; a second dolly surface generally conforming to the shape of the first dolly surface; and a guide channel for guiding a digital imaging device along a specified path so as to acquire the plurality of two-dimensional image files of the target object.

Abstract: An automated tread registration system includes a sensing system configured to provide at least one surface model of a first object. The surface model of the first object has at least one pattern with at least one groove and at least one protrusion. The automated tread registration system also includes a user system executing processing software reading data corresponding to the at least one surface model of the first object. The surface model corresponds to a current condition of the first object. The processing software executed by the user system may analyze the at least one surface model of the first object and provide at least one indicative metric based on the analysis of the surface model. The indicative metric may be camber wear, toe wear, heel toe wear, overinflation, underinflation, and/or the like.

Abstract: Disclosed is a system and method for documenting and analyzing a tire tread using a guidance dolly for use in creating a three-dimensional image file of a target object from a plurality of two-dimensional image files including: a first dolly surface adapted to enable placement of the guidance dolly into physical contact with the target object; a second dolly surface generally conforming to the shape of the first dolly surface; and a guide channel for guiding a digital imaging device along a specified path so as to acquire the plurality of two-dimensional image files of the target object.

Abstract: Disclosed is a system and method for documenting and analyzing a tire tread using a guidance dolly for use in creating a three-dimensional image file of a target object from a plurality of two-dimensional image files including: a first dolly surface adapted to enable placement of the guidance dolly into physical contact with the target object; a second dolly surface generally conforming to the shape of the first dolly surface; and a guide channel for guiding a digital imaging device along a specified path so as to acquire the plurality of two-dimensional image files of the target object.

Abstract: A method for verifying the reliable operation of a fuel tank pressure transducer positioned within a fuel tank of a PHEV. The method detects the initiation of a refueling cycle, which leads to opening a system vent valve positioned between a carbon canister and a system vent, and opening a fuel tank isolation valve. A controller monitors fuel system pressure within the fuel tank and at the vent valve, and that device determines rates of change of the fuel system pressure within the fuel tank and at the outlet vent. Reliable operation of the fuel tank pressure transducer is indicated upon a determination that the rates of change of the fuel system pressure in the fuel tank and at the outlet vent are uniform within a predetermined range.

Abstract: A method for verifying the reliable operation of a fuel tank pressure transducer positioned within a fuel tank of a PHEV. The method detects the initiation of a refueling cycle, which leads to opening a system vent valve positioned between a carbon canister and a system vent, and opening a fuel tank isolation valve. A controller monitors fuel system pressure within the fuel tank and at the vent valve, and that device determines rates of change of the fuel system pressure within the fuel tank and at the outlet vent. Reliable operation of the fuel tank pressure transducer is indicated upon a determination that the rates of change of the fuel system pressure in the fuel tank and at the outlet vent are uniform within a predetermined range.

Abstract: Disclosed is a system and method for documenting and analyzing a tire tread using a guidance dolly for use in creating a three-dimensional image file of a target object from a plurality of two-dimensional image files including: a first dolly surface adapted to enable placement of the guidance dolly into physical contact with the target object; a second dolly surface generally conforming to the shape of the first dolly surface; and a guide channel for guiding a digital imaging device along a specified path so as to acquire the plurality of two-dimensional image files of the target object.

Abstract: A percussion drilling assembly for boring into the earth is disclosed. The percussion drilling assembly includes a top sub, a casing coupled to the top sub, and a feed tube assembly disposed within the top sub and the casing. The feed tube assembly includes a non-rigid coupling having a throughbore and a tubular body disposed therein. The tubular body is translatable in the radial position and/or rotatable relative to a longitudinal centerline extending through the non-rigid coupling.

Abstract: A percussion drilling assembly for boring into the earth is disclosed. The percussion drilling assembly includes a top sub, a casing coupled to the top sub, and a feed tube assembly disposed within the top sub and the casing. The feed tube assembly includes a non-rigid coupling having a throughbore and a tubular body disposed therein. The tubular body is translatable in the radial position and/or rotatable relative to a longitudinal centerline extending through the non-rigid coupling.

Abstract: A percussion drilling assembly for boring into the earth is disclosed. The percussion drilling assembly includes a top sub, a casing coupled to the top sub, and a feed tube assembly disposed within the top sub and the casing. The feed tube assembly includes a non-rigid coupling having a throughbore and a tubular body disposed therein. The tubular body is translatable in the radial position and/or rotatable relative to a longitudinal centerline extending through the non-rigid coupling.

Abstract: A percussion drilling assembly for boring into the earth is disclosed. The percussion drilling assembly includes a top sub, a casing coupled to the top sub, and a feed tube assembly disposed within the top sub and the casing. The feed tube assembly includes a non-rigid coupling having a throughbore and a tubular body disposed therein. The tubular body is translatable in the radial position and/or rotatable relative to a longitudinal centerline extending through the non-rigid coupling.

Abstract: A method of generating intender leads in a distributed computer system includes the steps of identifying purchase indicators and extracting prospect identifiers from the purchase indicators. Purchase indicators are pieces of data that represent a potential future purchase by a prospect. For example, an online classified advertisement selling an automobile is a purchase indicator for a potential future purchase of a new car by the old car seller. The prospect identifier, such as a telephone number or email address, uniquely identifies the prospect likely to make the future purchase. Preferably, the method also contains the steps of obtaining full contact information for the prospect from a profile database, applying a predictive model to the prospects to select intender leads, and transferring the intender leads to an interested party, such as a direct marketing service or sales force.

Abstract: A method for encapsulating a photovoltaic module and an encapsulated photovoltaic module. The photovoltaic module includes a superstrate and one or more photovoltaic cells disposed on the superstrate. A barrier coating is applied to the exposed side of the photovoltaic module. The barrier coating is a mixture of a two component fluorinated polyurethane with 3-glycidoxy propyltrimethoxy silane. The photovoltaic module may also include an isolation scribe located around the perimeter of the exposed surface of the module.