Patents by Inventor Jacob Charles Carlson
Jacob Charles Carlson has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20220195418Abstract: The invention provides systems, methods, reagents, apparatuses, vectors, and host cells for the continuous evolution of nucleic acids. For example, a lagoon is provided in which a population of viral vectors comprising a gene of interest replicates in a stream of host cells, wherein the viral vectors lack a gene encoding a protein required for the generation of infectious viral particles, and wherein that gene is expressed in the host cells under the control of a conditional promoter, the activity of which depends on a function of the gene of interest to be evolved. Some aspects of this invention provide evolved products obtained from continuous evolution procedures described herein. Kits containing materials for continuous evolution are also provided.Type: ApplicationFiled: December 3, 2021Publication date: June 23, 2022Applicant: President and Fellows of Harvard CollegeInventors: David R. Liu, Kevin Michael Esvelt, Jacob Charles Carlson
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Patent number: 11214792Abstract: The invention provides systems, methods, reagents, apparatuses, vectors, and host cells for the continuous evolution of nucleic acids. For example, a lagoon is provided in which a population of viral vectors comprising a gene of interest replicates in a stream of host cells, wherein the viral vectors lack a gene encoding a protein required for the generation of infectious viral particles, and wherein that gene is expressed in the host cells under the control of a conditional promoter, the activity of which depends on a function of the gene of interest to be evolved. Some aspects of this invention provide evolved products obtained from continuous evolution procedures described herein. Kits containing materials for continuous evolution are also provided.Type: GrantFiled: May 13, 2019Date of Patent: January 4, 2022Assignee: President and Fellows of Harvard CollegeInventors: David R. Liu, Kevin Michael Esvelt, Jacob Charles Carlson
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Publication number: 20190276816Abstract: The invention provides systems, methods, reagents, apparatuses, vectors, and host cells for the continuous evolution of nucleic acids. For example, a lagoon is provided in which a population of viral vectors comprising a gene of interest replicates in a stream of host cells, wherein the viral vectors lack a gene encoding a protein required for the generation of infectious viral particles, and wherein that gene is expressed in the host cells under the control of a conditional promoter, the activity of which depends on a function of the gene of interest to be evolved. Some aspects of this invention provide evolved products obtained from continuous evolution procedures described herein. Kits containing materials for continuous evolution are also provided.Type: ApplicationFiled: May 13, 2019Publication date: September 12, 2019Applicant: President and Fellows of Harvard CollegeInventors: David R. Liu, Kevin Michael Esvelt, Jacob Charles Carlson
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Publication number: 20190256842Abstract: Strategies, systems, methods, reagents, and kits for phage-assisted continuous evolution are provided herein. These include strategies, systems, methods, reagents, and kits allowing for stringency modulation to evolve weakly active or inactive biomolecule variants, negative selection of undesired properties, and/or positive selection of desired properties.Type: ApplicationFiled: January 2, 2019Publication date: August 22, 2019Applicant: President and Fellows of Harvard CollegeInventors: David R. Liu, Jacob Charles Carlson, Ahmed Hussein Badran, Kevin Michael Esvelt
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Patent number: 10336997Abstract: The invention provides systems, methods, reagents, apparatuses, vectors, and host cells for the continuous evolution of nucleic acids. For example, a lagoon is provided in which a population of viral vectors comprising a gene of interest replicates in a stream of host cells, wherein the viral vectors lack a gene encoding a protein required for the generation of infectious viral particles, and wherein that gene is expressed in the host cells under the control of a conditional promoter, the activity of which depends on a function of the gene of interest to be evolved. Some aspects of this invention provide evolved products obtained from continuous evolution procedures described herein. Kits containing materials for continuous evolution are also provided.Type: GrantFiled: June 21, 2016Date of Patent: July 2, 2019Assignee: President and Fellows of Harvard CollegeInventors: David R. Liu, Kevin Michael Esvelt, Jacob Charles Carlson
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Patent number: 10179911Abstract: Strategies, systems, methods, reagents, and kits for phage-assisted continuous evolution are provided herein. These include strategies, systems, methods, reagents, and kits allowing for stringency modulation to evolve weakly active or inactive biomolecule variants, negative selection of undesired properties, and/or positive selection of desired properties.Type: GrantFiled: January 20, 2015Date of Patent: January 15, 2019Assignee: President and Fellows of Harvard CollegeInventors: David R. Liu, Jacob Charles Carlson, Ahmed Hussein Badran, Kevin Michael Esvelt
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Publication number: 20170009224Abstract: The invention provides systems, methods, reagents, apparatuses, vectors, and host cells for the continuous evolution of nucleic acids. For example, a lagoon is provided in which a population of viral vectors comprising a gene of interest replicates in a stream of host cells, wherein the viral vectors lack a gene encoding a protein required for the generation of infectious viral particles, and wherein that gene is expressed in the host cells under the control of a conditional promoter, the activity of which depends on a function of the gene of interest to be evolved. Some aspects of this invention provide evolved products obtained from continuous evolution procedures described herein. Kits containing materials for continuous evolution are also provided.Type: ApplicationFiled: June 21, 2016Publication date: January 12, 2017Applicant: President and Fellows of HarvardInventors: David R. Liu, Kevin Michael Esvelt, Jacob Charles Carlson
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Publication number: 20160348096Abstract: Strategies, systems, methods, reagents, and kits for phage-assisted continuous evolution are provided herein. These include strategies, systems, methods, reagents, and kits allowing for stringency modulation to evolve weakly active or inactive biomolecule variants, negative selection of undesired properties, and/or positive selection of desired properties.Type: ApplicationFiled: January 20, 2015Publication date: December 1, 2016Applicant: President and Fellows of Harvard CollegeInventors: David R. Liu, Jacob Charles Carlson, Ahmed Hussein Badran, Kevin Michael Esvelt
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Patent number: 9394537Abstract: The invention provides systems, methods, reagents, apparatuses, vectors, and host cells for the continuous evolution of nucleic acids. For example, a lagoon is provided in which a population of viral vectors comprising a gene of interest replicates in a stream of host cells, wherein the viral vectors lack a gene encoding a protein required for the generation of infectious viral particles, and wherein that gene is expressed in the host cells under the control of a conditional promoter, the activity of which depends on a function of the gene of interest to be evolved. Some aspects of this invention provide evolved products obtained from continuous evolution procedures described herein. Kits containing materials for continuous evolution are also provided.Type: GrantFiled: December 22, 2011Date of Patent: July 19, 2016Assignee: President and Fellows of Harvard CollegeInventors: David R. Liu, Kevin Michael Esvelt, Jacob Charles Carlson
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Publication number: 20130345064Abstract: The invention provides systems, methods, reagents, apparatuses, vectors, and host cells for the continuous evolution of nucleic acids. For example, a lagoon is provided in which a population of viral vectors comprising a gene of interest replicates in a stream of host cells, wherein the viral vectors lack a gene encoding a protein required for the generation of infectious viral particles, and wherein that gene is expressed in the host cells under the control of a conditional promoter, the activity of which depends on a function of the gene of interest to be evolved. Some aspects of this invention provide evolved products obtained from continuous evolution procedures described herein. Kits containing materials for continuous evolution are also provided.Type: ApplicationFiled: December 22, 2011Publication date: December 26, 2013Applicant: President and Fellows of Harvard CollegeInventors: David R. Liu, Kevin Michael Esvelt, Jacob Charles Carlson