Patents by Inventor Jacob Freudenthal
Jacob Freudenthal has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20230304932Abstract: A system (1000) comprising first and second excitation sources (101a, 101b) with respective excitation wavelengths for exciting first second and third dyes in a sample (110) and further comprising a detector (115), first and second emission spectral elements (121a, 121b) for transmitting respective first and second emission wavelengths as well as a processor (130) for automatically operating the elements of the system. The first dye comprises a first absorption spectrum comprising a first maximum absorption wavelength and the second dye comprises a second absorption spectrum comprising a second maximum absorption wavelength that is equal to or substantially equal to the first maximum absorption wavelength. The second dye comprises a second emission spectrum comprising a second maximum emission wavelength and the third dye comprises a third emission spectrum comprising a third maximum emission wavelength that is equal to or substantially equal to the second maximum emission wavelength.Type: ApplicationFiled: July 23, 2021Publication date: September 28, 2023Inventors: Scott BENSON, Steven MENCHEN, Chu-An CHANG, Linda LEE, Jacob FREUDENTHAL, Khairuzzaman MULLAH
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Publication number: 20230296513Abstract: Energy transfer dye pairs including a donor dye covalently attached to an acceptor dye through a linker, uses of the energy transfer dye pairs, for example, in conjugates of an energy transfer dye pair covalently attached to a quencher and an analyte (e.g., an oligonucleotide), for biological applications including, for example, amplification assays such as quantitative polymerase chain reaction (qPCR) and digital polymerase chain reaction (dPCR). Systems and methods include those in which (1) two dyes have the same excitation wavelength range, but different emission wavelength ranges and/or (2) two dyes have the same emission wavelength range, but different excitation wavelength ranges.Type: ApplicationFiled: July 23, 2021Publication date: September 21, 2023Inventors: Jacob FREUDENTHAL, Scott BENSON
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Patent number: 10648912Abstract: In one exemplary embodiment, a method for validating an instrument is provided. The method includes receiving amplification data from a validation plate to generate a plurality of amplification curves. The validation plate includes a sample of a first quantity and a second quantity, and each amplification curve includes an exponential region. The method further includes determining a set of fluorescence thresholds based on the exponential regions of the plurality of amplification curves and determining, for each fluorescence threshold of the set, a first set of cycle threshold (Ct) values of amplification curves generated from the samples of the first quantity and a second set of Ct values of amplification curves generated from the samples of the second quantity. The method includes calculating if the first and second quantities are sufficiently distinguishable based on Ct values at each of the plurality of fluorescence thresholds.Type: GrantFiled: February 5, 2016Date of Patent: May 12, 2020Assignee: Life Technologies CorporationInventors: Thomas Wessel, Yong Chu, Jacob Freudenthal, David Woo
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Patent number: 10501788Abstract: Some embodiments describe a computer-implemented method for calibrating a fluorescent dye. The method can comprise imaging a sample holder, loaded into an instrument, at more than one channel. The sample holder can comprise a plurality of reaction sites and more than one dye type, with each dye occupying more than one reaction site. The method can further comprise identifying a peak channel for each dye on the sample holder, normalizing each channel to the peak channel for each dye, and producing a dye matrix that can comprise a set of dye reference values.Type: GrantFiled: February 5, 2016Date of Patent: December 10, 2019Assignee: Life Technologies CorporationInventors: Yong Chu, Jacob Freudenthal, Jeffrey Marks
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Patent number: 10385383Abstract: A case for containing a plurality of biological samples contains a base, a substrate and a cover. The substrate is attached to the base and comprises a plurality of reaction regions configured to receive one or more biological samples. The cover comprises an inner surface and is configured to be attached to the base such that the base and cover together provide a cavity containing the substrate. The inner surface includes a central area and a recessed area disposed about the central area. The central area is configured to cover the reaction regions, while the recessed area is offset from the central area in a direction normal to the inner surface.Type: GrantFiled: February 19, 2016Date of Patent: August 20, 2019Assignee: Life Technologies CorporationInventors: Ronald Danehy, Gary Lim, Jacob Freudenthal
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Publication number: 20190126281Abstract: A biological analysis system is provided. The system comprises a sample block assembly. The sample block assembly comprises a sample block configured to accommodate a sample holder, the sample holder configured to receive a plurality of samples. The system also comprises a control system configured to cycle the plurality of samples through a series of temperatures. The system further comprises an automated tray comprising a slide assembly, the tray configured to reversibly slide the sample block assembly from a closed to an open position to allow user access to the plurality of sample holders.Type: ApplicationFiled: August 29, 2018Publication date: May 2, 2019Inventors: Yong CHU, Jeffrey MARKS, Jacob FREUDENTHAL, Mingsong CHEN, Tiong Han TOH, Mauro AGUANNO, Lik Seng LAU, Lian Seng LOH, Kok Siong TEO, Zeng Wei CHU, Xin MATHERS, Michael UY, Huei Steven YEO, Kuan Moon BOO, Way Xuang LEE, Chin Yong KOO, Wei Fuh TEO, Soo Yong LAU, Hon Siu SHIN, Zeqi TAN, Thomas WESSEL, David WOO
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Patent number: 10147182Abstract: A method for calibrating a biological instrument is provided. The method comprises the steps of acquiring an image of at least one biological sample array, determining a first region of interest within the image, wherein the first region of interest comprises a first plurality of locations on the at least one biological array; and identifying within the first region of interest, a plurality of image elements associated with each of the first plurality of locations on the at least one biological array.Type: GrantFiled: November 14, 2016Date of Patent: December 4, 2018Assignee: LIFE TECHNOLOGIES CORPORATIONInventors: Jacob Freudenthal, Jeffrey Marks, Francis Cheng, Thomas Wessel
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Publication number: 20180292320Abstract: In one exemplary embodiment, a method for calibrating an instrument is provided. The instrument includes an optical system capable of imaging fluorescence emission from a plurality of reaction sites. The method includes performing a region-of-interest (ROI) calibration to determine reaction site positions in an image. The method further includes performing a pure dye calibration to determine the contribution of a fluorescent dye used in each reaction site by comparing a raw spectrum of the fluorescent dye to a pure spectrum calibration data of the fluorescent dye. The method further includes performing an instrument normalization calibration to determine a filter normalization factor. The method includes performing an RNase P validation to validate the instrument is capable of distinguishing between two different quantities of sample.Type: ApplicationFiled: June 15, 2018Publication date: October 11, 2018Applicant: Life Technologies CorporationInventors: Yong Chu, Jeffrey Marks, Jacob Freudenthal, Thomas Wessel, David Woo
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Patent number: 10012590Abstract: In one exemplary embodiment, a method for calibrating an instrument is provided. The instrument includes an optical system capable of imaging florescence emission from a plurality of reaction sites. The method includes performing a region-of-interest (ROI) calibration to determine reaction site positions in an image. The method further includes performing a pure dye calibration to determine the contribution of a fluorescent dye used in each reaction site by comparing a raw spectrum of the fluorescent dye to a pure spectrum calibration data of the fluorescent dye. The method further includes performing an instrument normalization calibration to determine a filter normalization factor. The method includes performing an RNase P validation to validate the instrument is capable of distinguishing between two different quantities of sample.Type: GrantFiled: February 5, 2016Date of Patent: July 3, 2018Assignee: Life Technolgies CorporationInventors: Yong Chu, Jeffrey Marks, Jacob Freudenthal, Thomas Wessel, David Woo
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Patent number: 9702823Abstract: An instrument for processing and/or measuring a biological process contains an excitation source, a sample holder, an optical sensor, an excitation optical system, and an emission optical system. The sample holder is configured to receive a plurality of biological samples. The optical sensor is configured to receive an emission from the biological samples. The excitation optical system is disposed along an excitation optical path and is configured to direct the electromagnetic radiation from the excitation source to the biological samples. The emission optical system is disposed along an emission optical path and is configured to direct electromagnetic emissions from the biological samples to the optical sensor. The instrument further contains a plurality of filter assemblies configured to be interchangeably located along at least one of the optical paths.Type: GrantFiled: September 28, 2012Date of Patent: July 11, 2017Assignee: LIFE TECHNOLOGIES CORPORATIONInventors: Kevin Maher, Mingsong Chen, Jacob Freudenthal, Kuan Moon Boo, Soo Yong Lau, David Fortescue, Ming Shen, Woon Liang Terence Soh
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Publication number: 20170161429Abstract: A method for calibrating a biological instrument is provided. The method comprises the steps of acquiring an image of at least one biological sample array, determining a first region of interest within the image, wherein the first region of interest comprises a first plurality of locations on the at least one biological array; and identifying within the first region of interest, a plurality of image elements associated with each of the first plurality of locations on the at least one biological array.Type: ApplicationFiled: November 14, 2016Publication date: June 8, 2017Inventors: Jacob Freudenthal, Jeffrey Marks, Francis Cheng, Thomas Wessel
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Publication number: 20160228876Abstract: A biological analysis system is provided. The system comprises a sample block assembly. The sample block assembly comprises a sample block configured to accommodate a sample holder, the sample holder configured to receive a plurality of samples. The system also comprises a control system configured to cycle the plurality of samples through a series of temperatures. The system further comprises an automated tray comprising a slide assembly, the tray configured to reversibly slide the sample block assembly from a closed to an open position to allow user access to the plurality of sample holders.Type: ApplicationFiled: February 5, 2016Publication date: August 11, 2016Inventors: Yong Chu, Jeffrey Marks, Jacob Freudenthal, Mingsong Chen, Tiong Han Toh, Mauro Aguanno, Lik Seng Lau, Lian Seng Loh, Kok Siong Teo, Zeng Wei Chu, Xin Mathers, Michael Uy, Huei Yeo, Kuan Moon Boo, Way Xuang Lee, Chin Yong Koo, Wei Fuh Teo, Soo Yong Lau, Hon Siu Shin, Zeqi Tan, Thomas Wessel, Woo David
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Publication number: 20160231245Abstract: In one exemplary embodiment, a method for validating an instrument is provided. The method includes receiving amplification data from a validation plate to generate a plurality of amplification curves. The validation plate includes a sample of a first quantity and a second quantity, and each amplification curve includes an exponential region. The method further includes determining a set of fluorescence thresholds based on the exponential regions of the plurality of amplification curves and determining, for each fluorescence threshold of the set, a first set of cycle threshold (Ct) values of amplification curves generated from the samples of the first quantity and a second set of Ct values of amplification curves generated from the samples of the second quantity. The method includes calculating if the first and second quantities are sufficiently distinguishable based on Ct values at each of the plurality of fluorescence thresholds.Type: ApplicationFiled: February 5, 2016Publication date: August 11, 2016Inventors: Thomas Wessel, Yong Chu, Jacob Freudenthal, David Woo
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Publication number: 20160230209Abstract: Some embodiments describe a computer-implemented method for calibrating a fluorescent dye. The method can comprise imaging a sample holder, loaded into an instrument, at more than one channel. The sample holder can comprise a plurality of reaction sites and more than one dye type, with each dye occupying more than one reaction site. The method can further comprise identifying a peak channel for each dye on the sample holder, normalizing each channel to the peak channel for each dye, and producing a dye matrix that can comprise a set of dye reference values.Type: ApplicationFiled: February 5, 2016Publication date: August 11, 2016Inventors: Yong Chu, Jacob Freudenthal, Jeffrey Marks
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Publication number: 20160231246Abstract: In one exemplary embodiment, a method for calibrating an instrument is provided. The instrument includes an optical system capable of imaging florescence emission from a plurality of reaction sites. The method includes performing a region-of-interest (ROI) calibration to determine reaction site positions in an image. The method further includes performing a pure dye calibration to determine the contribution of a fluorescent dye used in each reaction site by comparing a raw spectrum of the fluorescent dye to a pure spectrum calibration data of the fluorescent dye. The method further includes performing an instrument normalization calibration to determine a filter normalization factor. The method includes performing an RNase P validation to validate the instrument is capable of distinguishing between two different quantities of sample.Type: ApplicationFiled: February 5, 2016Publication date: August 11, 2016Inventors: Yong Chu, Jeffrey Marks, Jacob Freudenthal, Thomas Wessel, David Woo
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Publication number: 20160168626Abstract: A case for containing a plurality of biological samples contains a base, a substrate and a cover. The substrate is attached to the base and comprises a plurality of reaction regions configured to receive one or more biological samples. The cover comprises an inner surface and is configured to be attached to the base such that the base and cover together provide a cavity containing the substrate. The inner surface includes a central area and a recessed area disposed about the central area. The central area is configured to cover the reaction regions, while the recessed area is offset from the central area in a direction normal to the inner surface.Type: ApplicationFiled: February 19, 2016Publication date: June 16, 2016Inventors: Ronald DANEHY, Gary LIM, Jacob FREUDENTHAL
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Patent number: 9267170Abstract: A case for containing a plurality of biological samples contains a base, a substrate and a cover. The substrate is attached to the base and comprises a plurality of reaction regions configured to receive one or more biological samples. The cover comprises an inner surface and is configured to be attached to the base such that the base and cover together provide a cavity containing the substrate. The inner surface includes a central area and a recessed area disposed about the central area. The central area is configured to cover the reaction regions, while the recessed area is offset from the central area in a direction normal to the inner surface.Type: GrantFiled: September 28, 2012Date of Patent: February 23, 2016Assignee: Life Technologies CorporationInventors: Ronald Danehy, Gary Lim, Jacob Freudenthal
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Publication number: 20140242596Abstract: A case for containing a plurality of biological samples contains a base, a substrate and a cover. The substrate is attached to the base and comprises a plurality of reaction regions configured to receive one or more biological samples. The cover comprises an inner surface and is configured to be attached to the base such that the base and cover together provide a cavity containing the substrate. The inner surface includes a central area and a recessed area disposed about the central area. The central area is configured to cover the reaction regions, while the recessed area is offset from the central area in a direction normal to the inner surface.Type: ApplicationFiled: September 28, 2012Publication date: August 28, 2014Inventors: Ronald Danehy, Gary Lim, Jacob Freudenthal
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Publication number: 20070207065Abstract: Microfluidic devices, assemblies, and systems are provided, as are methods of manipulating micro-sized samples of fluids. Microfluidic devices having a plurality of specialized processing features are also provided.Type: ApplicationFiled: April 30, 2007Publication date: September 6, 2007Applicant: Applera CorporationInventors: Sean Desmond, Zbigniew Bryning, John Shigeura, Gary Lim, Adrian Fawcett, Jacob Freudenthal, Gary Bordenkircher