Abstract: The apparatus for detecting chemiluminescent light is a component of a luminometer that can be incorporated in a sample analysis system. Such apparatus, also referred to as a fiber optic read-head, includes a fiber optic cable having a light-transmission end that connects to a photon counter of the luminometer. An opposite end portion of the fiber optic cable is divided into a plurality of fiber optic ribbons. Each of the fiber optic ribbons has a light-receiving end. The ribbons are radially arranged such that the light-receiving ends define a cylindrical passageway for accommodation of a sample analysis tube or cuvette. The fiber optic ribbons are maintained in their desired position by a core. The core is housed in an opaque housing that prevents light from coming in or straying out.

Abstract: A method of composition is featured for performing a fluoroimmunoassay of a biological fluid sample for an immunological reactant. A conjugate of a carrier labelled with fluorophores and coupled to an immunological reactant is mixed with the sample and a known quantity of binding agent. After equilibrium is accomplished, the bound and unbound portions are separated. The carrier in a selected portion is chemically treated to liberate the fluorophores. The fluorescent level is then measured and compared with a standard.

Abstract: Apparatus and method for determining the concentration of a constituent in a sample using chromatographic and optical measuring techniques. A sample containing a constituent to be analyzed is fractionated by a chromatographic column. A light beam is passed through the constituent fraction in the column, and the light absorbance (or transmittance) is measured. The fluid carrier (or solvent) eluting through the column will generally increase the transmissivity of the light passing through the column due to a difference between the indices of refraction of the carrier fluid and the chromatographic packing material. Such increased transmissivity will generally offset any decrease in the transmittance due to trace amounts of the constituent in the sample. The optical measurement is compensated to nullify such increased transmissivity, so as to allow measurement of such trace amounts of constituents.