Abstract: A mesoscale fluidic system comprises a substrate having a sample chamber and an analysis chamber. The sample chamber comprises a cell permeable filter defining a sample application compartment and a conditioning medium compartment. The sample chamber has a sample inlet port in the sample application compartment. The analysis chamber has an entry port and an exit port. The conditioning medium compartment is in fluid communication with the entry port of the analysis chamber via a channel. The sample application compartment is below the cell permeable filter and the conditioning medium compartment is above the cell permeable filter. The mesoscale fluidic system is suited for analysing cellular motility in a sample. Also disclosed is a method of estimating the quantity of motile cells in a sample and a method of extracting motile cells from non-motile cells.

Abstract: A mesoscale fluidic system comprises a substrate having a sample chamber and an analysis chamber. The sample chamber comprises a cell permeable filter defining a sample application compartment and a conditioning medium compartment. The sample chamber has a sample inlet port in the sample application compartment. The analysis chamber has an entry port and an exit port. The conditioning medium compartment is in fluid communication with the entry port of the analysis chamber via a channel. The sample application compartment is below the cell permeable filter and the conditioning medium compartment is above the cell permeable filter. The mesoscale fluidic system is suited for analysing cellular motility in a sample. Also disclosed is a method of estimating the quantity of motile cells in a sample and a method of extracting motile cells from non-motile cells.

Abstract: A mesoscale fluidic system comprises a substrate having a sample chamber and an analysis chamber. The sample chamber comprises a cell permeable filter defining a sample application compartment and a conditioning medium compartment. The sample chamber has a sample inlet port in the sample application compartment. The analysis chamber has an entry port and an exit port. The conditioning medium compartment is in fluid communication with the entry port of the analysis chamber via a channel. The sample application compartment is below the cell permeable filter and the conditioning medium compartment is above the cell permeable filter. The mesoscale fluidic system is suited for analysing cellular motility in a sample. Also disclosed is a method of estimating the quantity of motile cells in a sample and a method of extracting motile cells from non-motile cells.

Abstract: A mesoscale fluidic system comprises a substrate having a sample chamber and an analysis chamber. The sample chamber comprises a cell permeable filter defining a sample application compartment and a conditioning medium compartment. The sample chamber has a sample inlet port in the sample application compartment. The analysis chamber has an entry port and an exit port. The conditioning medium compartment is in fluid communication with the entry port of the analysis chamber via a channel. The sample application compartment is below the cell permeable filter and the conditioning medium compartment is above the cell permeable filter. The mesoscale fluidic system is suited for analysing cellular motility in a sample. Also disclosed is a method of estimating the quantity of motile cells in a sample and a method of extracting motile cells from non-motile cells.

Abstract: Disclosed herein is mesoscale bioreactor platform comprising an upwards open chamber for a biological cell, which chamber via a first port is in communication with a first channel for conducting an influent stream of a liquid into the chamber and via a second port is in communication with a second channel for conducting an effluent stream of a liquid away from the chamber, which chamber is provided with a closure comprising a water-immiscible liquid, and wherein said first channel is in fluid communication with a reservoir for a liquid and said second channel is in fluid communication with a waste container. Furthermore, a method for modifying the interaction of a content of a chamber with the surroundings is described as well as method of culturing a biological cell.

Abstract: Disclosed herein is a cell culturing system comprising a culturing chamber for culturing a biological cell in a growth medium and a sensor for measuring a signal in the spent growth medium, wherein the culturing chamber is provided in a mesoscale bioreactor platform with an inlet opening for an influent stream of growth medium and a outlet opening for an effluent stream of spent growth medium, said outlet opening, said spent growth medium being in fluid communication with a sample port for releasable adoption of the sensor. Furthermore, a method of measuring an effluent stream of spent growth medium from a culturing chamber in the cell culturing system is disclosed.

Abstract: Disclosed is a mesoscale bioreactor platform including two or more liquid reservoirs in fluid communication with a culture chamber which chamber is in fluid communication with an exit. The platform allows the chamber to be perfused with a flow of liquid from one or more of the liquid reservoirs. The integrated reservoirs for liquids remove the need for external supplies of liquid to the culture chamber during cell culture experiments requiring perfusion of liquids. Moreover, with two or more reservoir it is possible to supply the culture chamber with different types of liquids.

Abstract: A culture dish (1) comprising a basic structure (2) with a number of macro wells (4) for culturing oocytes and embryos, the culture dish further comprising a lid (3) with a movable part (8), the movable part (8) having an opening (11) adapted to the size of the macro wells (4), the movable part (8) being movable between a first position where all macro wells are closed by the lid (3) and further positions where for each further position (11) the opening (11) is aligned with one of the macro wells (4) to allow access to this macro well (4) through the opening (11) in the lid (3).