Abstract: A device and method for generating in-situ microneedles in a subject. The device includes a body, a microneedle coupled to the body, a reservoir coupled to the body, a first motor. The microneedle is positioned within a chamber at a distal end of the body. The reservoir includes a biomaterial fluid and is in fluid communication with the microneedle. The first motor is configured to activate the reservoir to expel the biomaterial fluid to the microneedle. The device also includes a temperature control assembly coupled to the reservoir and configured to set and maintain a temperature of the reservoir, a second motor coupled to the body and the microneedle, a microneedle size device coupled to the microneedle and configured to set a length of the microneedle extending from the chamber.

Abstract: A multi-material printer can include a housing that is configured to be handheld. The housing can be configured to receive first and second printable materials from respective first and second containers. A nozzle can define an outlet of the multi-material printer. At least one actuator can be configured to cause first and second printable materials from the first and second containers to flow at a respective constant rate. The multi-material printer can be configured to simultaneously extrude the first and second printable materials from the outlet. The multi-material printer can be used for fabricating a scaffold from filaments comprising the first and second materials directly within the body of a human or animal.

Abstract: Disclosed are devices, systems and methods that utilize bioprinters to form scaffold structures in situ to facilitate treatment of the musculoskeletal or skin disorders in patients. A method for treating a musculoskeletal disorder of a patient includes positioning a bioprinter at a situs of the musculoskeletal disorder, or a situs of a skin injury, in the patient. The method includes extruding a hydrogel formulation from the bioprinter into the situs. The method includes curing the hydrogel formulation in the situs. The hydrogel formulation has a composition effective to generate, upon the curing, a scaffold structure in the situs to facilitate muscle hypertrophy, or skin wound healing, in the situs.