Patents by Inventor Jacob R. Hartman

Jacob R. Hartman has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 6610520
    Abstract: A double-stranded cDNA molecule which includes DNA encoding human manganese superoxide dismutase has been created. The sequence of one strand of a double-stranded DNA molecule which encodes human manganese superoxide dismutase has been discovered. Such molecules may be introduced in procaryotic, e.g., bacterial, or eukaryotic, e.g., yeast or mammalian, cells and the resulting cells cultured or grown under suitable conditions so as to produce human manganese superoxide dismutase or analogs thereof which may then be recovered. By this invention, human MnSOD gene fragments from various plasmids may be ligated to yield a complete genomic human MnSOD gene fragment. Human MnSOD or analogs thereof may be used to catalyze the reduction of superoxide radicals, reduce reperfusion injury, prolong the survival time of isolated organs, or treat inflammations.
    Type: Grant
    Filed: August 31, 1994
    Date of Patent: August 26, 2003
    Assignee: Bio-Technology General Corp.
    Inventors: Jacob R. Hartman, Yaffa Beck
  • Patent number: 6361772
    Abstract: A double-stranded cDNA molecule which includes DNA encoding human manganese superoxide dismutase has been created. The sequence of one strand of a double-stranded DNA molecule which encodes human manganese superoxide dismutase has been discovered. Such molecules may be introduced in procaryotic, e.g., bacterial, or eukaryotic, e.g., yeast or mammalian, cells and the resulting cells cultured or grown under suitable conditions so as to produce human manganese superoxide dismutase or analogs thereof which may then be recovered. By this invention, human MnSOD gene fragments from various plasmids may be ligated to yield a complete genomic human MnSOD gene fragment. Human MnSOD or analogs thereof may be used to catalyze the reduction of superoxide radicals, reduce reperfusion injury, prolong the survival time of isolated organs, or treat inflammations.
    Type: Grant
    Filed: July 25, 1996
    Date of Patent: March 26, 2002
    Assignee: Bio-Technology General Corp.
    Inventors: Jacob R. Hartman, Yaffa Beck
  • Patent number: 6001604
    Abstract: An improved and efficient process for the production of recombinant human insulin by folding of a proinsulin hybrid polypeptide is provided.
    Type: Grant
    Filed: November 12, 1997
    Date of Patent: December 14, 1999
    Assignee: Bio-Technology General Corp.
    Inventors: Jacob R. Hartman, Simona Mendelovitz, Marian Gorecki
  • Patent number: 5540911
    Abstract: This invention provides plasmids for expression of human superoxide dismutase of analogs thereof. The invention further provides a method of producing enzymatically active human MnSOD or an analog thereof by introducing plasmids for expression of human MnSOD into procaryotic or eucaryotic cells and growing the resulting cells under suitable conditions, including conditions wherein the production medium is supplemented with Mn.sup.++. The invention additionally provides a method of recovering purified enzymatically active MnSOD from procaryotic and eucaryotic cells. Human MnSOD or analogs thereof may be used to reduce reperfusion injury, prolong the survival time of isolated organs or treat inflammations or bronchial pulmonary dysplasia.
    Type: Grant
    Filed: January 9, 1995
    Date of Patent: July 30, 1996
    Assignee: Bio-Technology General Corp.
    Inventors: Jacob R. Hartman, Yaffa Beck
  • Patent number: 5457042
    Abstract: A novel analog of human superoxide dismutase which comprises the non-acetylated, non-glycosylated form of natural human superoxide dismutase having the ser-met amino acid sequence attached to the N-terminus and a mixture comprising this analog and non-acetylated, non-glycosylated superoxide dismutase having an amino acid sequence identical to that of natural human superoxide dismutase are provided. Methods of catalyzing a chemical reaction using the novel analog and mixture are provided.
    Type: Grant
    Filed: August 21, 1992
    Date of Patent: October 10, 1995
    Assignee: Bio-Technology General Corp.
    Inventors: Jacob R. Hartman, Amos B. Oppenheim, Marian Gorecki, Haim Aviv, Rachel Oren
  • Patent number: 5455029
    Abstract: An improved plasmid for the production of superoxide dismutase on an analog thereof which upon introduction into a suitable host containing the thermolabile repressor C.sub.I renders the host capable of effecting expression of a gene encoding superoxide dismutase or the analog. The plasmid is a double-stranded DNA molecule which includes in 5' to 3' order the following: the promoter and operator P.sub.L O.sub.L from lambda bacteriophage; the N utilization site; a first restriction enzyme site permitting replacement of the ribosomal binding site which follows thereafter; a ribosomal binding site; an ATG initiation codon; a second restriction enzyme site; a gene encoding superoxide dismutase or the analog; an origin of replication and a gene associated with a selectable or identifiable phenotypic trait manifested when the plasmid is present in the host. The distance between the 3' end of P.sub.L O.sub.L and the 5' end of the N utilization site is less than about 80 base pairs.
    Type: Grant
    Filed: August 21, 1992
    Date of Patent: October 3, 1995
    Assignee: Bio-Technology General Corp.
    Inventors: Jacob R. Hartman, Amos B. Oppenheim, Marian Gorecki, Haim Aviv, Rachel Oren
  • Patent number: 5270195
    Abstract: An expression plasmid has been constructed which includes DNA encoding human manganese superoxide dismutase. Such plasmids may be introduced into host cells and the resulting cells cultured or grown under suitable conditions so as to produce enzymatically active human manganese superoxide dismutase or analogs thereof which may then be recovered. A method of producing an enzymatically active analog of human manganese superoxide dismutase by supplementing the culture media with Mn.sup.++ has been developed.
    Type: Grant
    Filed: July 10, 1992
    Date of Patent: December 14, 1993
    Assignee: Bio-Technology General Corp.
    Inventors: Jacob R. Hartman, Yaffa Beck
  • Patent number: 5246847
    Abstract: A double-stranded cDNA molecule which includes DNA encoding human manganese superoxide dismutase has been created. The sequence of one strand of a double-stranded DNA molecule which encodes human maganese superoxide dismutase has been discovered. Such molecules may be introduced in procaryotic, e.g., bacterial, or eukaryotic, e.g., yeast or mammalian, cells and the resulting cells cultured or grown under suitable conditions so as to produce human manganese superoxide dismutase or analogs thereof which may then be recovered. Human MnSOD or analogs thereof may be used to catalyze the reduction of superoxide radicals, reduce reperfusion injury, prolong the survival time of isolated organs, or treat inflammations.
    Type: Grant
    Filed: February 27, 1992
    Date of Patent: September 21, 1993
    Assignee: Bio-Technology General Corp.
    Inventors: Jacob R. Hartman, Yaffa Beck
  • Patent number: 5162217
    Abstract: An improved plasmid for the production of superoxide dismutase which upon introduction into a host containing the thermolabile repressor C.sub.I renders the host capable of effecting expression of a gene encoding superoxide dismutase. The plasmid which includes in 5' to 3' order the following: the promoter and operator P.sub.L O.sub.L from lambda bacteriophage; the N utilization site; a first restriction enzyme site permitting replacement of the ribosomal binding site which follows thereafter; a ribosomal binding site; an ATG initiation codon; a second restriction enzyme site; a gene encoding superoxide dismutase; an origin of replication and a gene associated with a selectable or identifiable phenotypic trait manifested when the plasmid is present in the host. The distance between the 3' end of P.sub.L O.sub.L and the 5' end of the N utilization site is less than about 80 base pairs.
    Type: Grant
    Filed: December 8, 1989
    Date of Patent: November 10, 1992
    Assignee: Bio-Technology General Corp.
    Inventors: Jacob R. Hartman, Amos B. Oppenheim, Marian Gorecki, Haim Aviv, Rachel Oren
  • Patent number: 5143836
    Abstract: An improved plasmid for the production of superoxide dismutase which upon introduction into a host containing the thermolabile repressor C.sub.I renders the host capable of effecting expression of a gene encoding superoxide dismutase. The plasmid includes in 5' to 3' order the following: the promoter and operator P.sub.L O.sub.L from lambda bacteriophage; the N utilization site; a first restriction enzyme site permitting replacement of the ribosomal binding site which follows thereafter; a ribosomal binding site; an ATG initiation codon; a second restriction enzyme site; a gene encoding superoxide dismutase; an origin of replication and a gene associated with a selectable or identifiable phenotypic trait manifested when the plasmid is present in the host. The distance between the 3' end of P.sub.L O.sub.L and the 5' end of the N utilization site is less than about 80 base pairs.
    Type: Grant
    Filed: May 13, 1988
    Date of Patent: September 1, 1992
    Assignee: Bio-Technology General Corp.
    Inventors: Jacob R. Hartman, Amos B. Oppenheim, Marian Gorecki, Haim Aviv
  • Patent number: 4742004
    Abstract: A method of producing enzymatically active eucaryotic superoxide dismutase or an analog thereof in a bacterial cell which contains and is capable of expressing a DNA sequence encoding the superoxide dismutase or analog thereof comprising maintaining the bacterial cell under suitable conditions and in a suitable production medium. The production medium is supplemented with an amount of Cu++ so that the concentration of Cu++ in the medium is greater than about about 2 ppm.The invention also concerns methods of recovering purified enzymatically active eucaryotic superoxide dismutase or analogs from bacterial cells producing the superoxide dismutase or analog.
    Type: Grant
    Filed: August 27, 1984
    Date of Patent: May 3, 1988
    Assignee: BIO-Technology General Corp.
    Inventors: Jacob R. Hartman, Dov Kanner, Daniel Bartfeld