Abstract: Provided herein, in some embodiments, are modular transcriptional architectures and methods for regulated expression of guide RNAs in cells, such as human cells, which are based on Clustered Regularly Interspaced Palindromic Repeats (CRISPR) systems.

Abstract: To manage energy demands of a plurality of appliances, a meter controller apparatus receives a first message from a first appliance controller apparatus coupled to a first appliance of the plurality of appliances. The first message comprises first information regarding a first power demand of the first appliance and a first demand category associated with the first appliance, wherein the first demand category is one of a plurality of demand categories. The meter controller apparatus computes an updated meter controller model for the plurality of appliances based at least in part on the first message received from the first appliance controller apparatus and a present meter controller model for the plurality of appliances.

Abstract: To manage energy demands of a plurality of appliances, a meter controller apparatus receives a first message from a first appliance controller apparatus coupled to a first appliance of the plurality of appliances. The first message comprises first information regarding a first power demand of the first appliance and a first demand category associated with the first appliance, wherein the first demand category is one of a plurality of demand categories. The meter controller apparatus computes an updated meter controller model for the plurality of appliances based at least in part on the first message received from the first appliance controller apparatus and a present meter controller model for the plurality of appliances.

Abstract: Methods, apparatuses and systems for managing energy demand of one or more appliances. In one example, an appliance controller apparatus is provided, comprising a user interface configured to receive a user input indicative of a demand flexibility associated with an appliance, a communication interface configured to receive a control input indicative of a request to modify a power demand of the appliance, and a processor programmed to determine whether the power demand of the appliance is to be modified based on the user input and the control input. In a further aspect, a meter controller apparatus is provided, comprising a communication interface configured to receive a message from an appliance controller apparatus coupled to an appliance, the message comprising information regarding a power demand of the appliance and a demand category associated with the appliance.

Abstract: Methods, apparatuses and systems for managing energy demand of one or more appliances. In one example, an appliance controller apparatus is provided, comprising a user interface configured to receive a user input indicative of a demand flexibility associated with an appliance, a communication interface configured to receive a control input indicative of a request to modify a power demand of the appliance, and a processor programmed to determine whether the power demand of the appliance is to be modified based on the user input and the control input. In a further aspect, a meter controller apparatus is provided, comprising a communication interface configured to receive a message from an appliance controller apparatus coupled to an appliance, the message comprising information regarding a power demand of the appliance and a demand category associated with the appliance.