Abstract: Disclosed are microfluidic flow-based electroporation systems that have a flow device, an electrical control module, a fluid delivery module, and a multi-well module. The systems can be used in methods of selecting an electroporation parameter, and in methods of electroporating cells using the selected parameters.

Abstract: Disclosed are flow devices and methods for electroporation, which allow controlling the throughput of electroporation, for example by operating the electroporation process at selected throughput or operating at an increased or decreased level of throughput compared to a reference level of throughput by scaling a subset of electroporation parameters, while allowing maintaining cell viability and transfection efficiency.