Abstract: A method of preparation of a potential herbal anti-cancer medicine from the leaves and twigs of Calotropis gigantean for prevention and treatment of all types of cancers, using fresh extract of leaves and twigs of Calotropis gigantea fortified with unrefined sugarcane sugar (jaggery). The aqueous extract of leaves and twigs of Calotropis gigantea was desiccated. The [3 H]-Thymidine growth assay was performed against cancer cell lines, WM 902-B and WM1341-D. The extract [28 mgms] was dissolved in 2 ml control at 0.5%, 0.1% DMSO. The extract is highly inhibitory at all dilutions and highly inhibitory to both the cancer cell lines.