Patents by Inventor Jacobus Thomas Pronk
Jacobus Thomas Pronk has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20200248194Abstract: The present invention relates to a yeast cell, in particular a recombinant yeast cell, the cell lacking enzymatic activity needed for the NADH-dependent glycerol synthesis or the cell having a reduced enzymatic activity with respect to the NADH-dependent glycerol synthesis compared to its corresponding wild-type yeast cell, the cell comprising one or more heterologous nucleic acid sequences encoding an NAD+-dependent acetylating acetaldehyde dehydrogenase (EC 1.2.1.10) activity. The invention further relates to the use of a cell according to the invention in the preparation of ethanol.Type: ApplicationFiled: March 27, 2020Publication date: August 6, 2020Inventors: Jacobus Thomas PRONK, Antonius Jeroen Adriaan VAN MARIS, Victor Gabriel GUADALUPE MEDINA
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Patent number: 10689670Abstract: The present invention describes a recombinant yeast cell functionally expressing one or more heterologous nucleic acid sequences encoding for ribulose-1,5-phosphate carboxylase/oxygenase (EC4.1.1.39; Rubisco), and optionally one or more molecular chaperones for Rubisco, and one or more phosphoribulokinase (EC2.7.1.19; PRK), wherein the phosphoribulokinase is under control of a promoter (the “PRK promoter”) that enables higher expression under anaerobic conditions than under aerobic conditions.Type: GrantFiled: June 13, 2017Date of Patent: June 23, 2020Assignee: DSM IP ASSETS B.V.Inventors: Ioannis Papapetridis, Antonius Jeroen Adriaan Van Maris, Jacobus Thomas Pronk, Paulus Petrus De Waal, Niels Lauret, Maarten Dirk Verhoeven, Jozef Petrus Johannes Schmitz
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Publication number: 20200181657Abstract: The present invention relates to a host cell which is capable of producing a dicarboxylic acid and which comprises at least one genetic modification in its genome resulting in the deficiency of at least one enzymatic step catalysing the oxidation of a cofactor. The invention also relates to a process for producing a dicarboxylic acid, which method comprises fermenting such a host cell in a suitable fermentation medium and producing the dicarboxylic acid.Type: ApplicationFiled: December 22, 2016Publication date: June 11, 2020Inventors: Alrik Pieter LOS, Zheng ZHAO, René VERWAAL, Jacobus Thomas PRONK, Rolf POLDERMANS
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Publication number: 20200157489Abstract: The present invention describes a recombinant yeast cell functionally expressing one or more heterologous nucleic acid sequences encoding for ribulose-1,5-phosphate carboxylase/oxygenase (EC4.1.1.39; Rubisco), and optionally one or more molecular chaperones for Rubisco, and one or more phosphoribulokinase (EC2.7.1.19; PRK), wherein one or more genes of the non-oxidative branch of the pentose phosphate pathway are overexpressed and/or wherein said yeast cell comprises a deletion or disruption of a glycerol-3-phosphate dehydrogenase (GPD) gene.Type: ApplicationFiled: June 4, 2018Publication date: May 21, 2020Inventors: Ioannis PAPAPETRIDIS, Jacobus Thomas PRONK
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Publication number: 20200095536Abstract: This invention relates to a recombinant cell, preferably a recombinant yeast cell comprising: a) a gene coding for an enzyme having glycerol-3-phosphate dehydrogenase activity, wherein said enzyme has a cofactor dependency for at least NADP+ and/or for NADPH; b) a gene encoding an enzyme having at least NAD+ dependent acetylating acetaldehyde dehydrogenase activity (EC 1.2.1.10); and c) a mutation or disruption in at least one gene selected from the group of GPD1 and GPD2. Said cell is suitable for ethanol production, has a reduced glycerol production at high ethanol yield.Type: ApplicationFiled: December 18, 2017Publication date: March 26, 2020Inventors: Ioannis PAPAPETRIDIS, Antonius Jeroen Adriaan VAN MARIS, Jacobus Thomas PRONK
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Patent number: 10533181Abstract: The present invention relates to a yeast cell, in particular a recombinant yeast cell, the cell lacking enzymatic activity needed for the NADH-dependent glycerol synthesis or the cell having a reduced enzymatic activity with respect to the NADH-dependent glycerol synthesis compared to its corresponding wild-type yeast cell, the cell comprising one or more heterologous nucleic acid sequences encoding an NAD+-dependent acetylating acetaldehyde dehydrogenase (EC 1.2.1.10) activity. The invention further relates to the use of a cell according to the invention in the preparation of ethanol.Type: GrantFiled: May 10, 2019Date of Patent: January 14, 2020Assignee: DSM IP ASSETS B.V.Inventors: Jacobus Thomas Pronk, Antonius Jeroen Adriaan Van Maris, Victor Gabriel Guadalupe Medina
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Publication number: 20190264217Abstract: The present invention relates to a yeast cell, in particular a recombinant yeast cell, the cell lacking enzymatic activity needed for the NADH-dependent glycerol synthesis or the cell having a reduced enzymatic activity with respect to the NADH-dependent glycerol synthesis compared to its corresponding wild-type yeast cell, the cell comprising one or more heterologous nucleic acid sequences encoding an NAD+-dependent acetylating acetaldehyde dehydrogenase (EC 1.2.1.10) activity. The invention further relates to the use of a cell according to the invention in the preparation of ethanol.Type: ApplicationFiled: May 10, 2019Publication date: August 29, 2019Inventors: Jacobus Thomas Pronk, Antonius Jeroen Adriaan Van Maris, Victor Gabriel Guadalupe Medina
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Publication number: 20190249201Abstract: The present invention describes a recombinant yeast cell functionally expressing one or more heterologous nucleic acid sequences encoding for ribulose-1,5-phosphate carboxylase/oxygenase (EC4.1.1.39; Rubisco), and optionally one or more molecular chaperones for Rubisco, and one or more phosphoribulokinase (EC2.7.1.19; PRK), wherein the phosphoribulokinase is under control of a promoter (the “PRK promoter”) that enables higher expression under anaerobic conditions than under aerobic conditions.Type: ApplicationFiled: June 13, 2017Publication date: August 15, 2019Inventors: Ioannis PAPAPETRIDIS, Antonius Jeroen Adriaan VAN MARIS, Jacobus Thomas PRONK, Paulus Petrus DE WAAL, Niels LAURET, Maarten Dirk VERHOEVEN, Jozef Petrus Johannes SCHMITZ
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Patent number: 10301633Abstract: The invention relates to a nucleic acid molecule encoding a novel selection marker. Said marker is a guanidinobutyrase from Kluyveromyces lactis, which, when expressed in Saccharomyces, allows the growth of the yeast in the presence of guanidinobutyrate as the sole nitrogen source. Said marker can be used in a method for producing a microorganism having an altered genome. The invention further relates to a set of constructs, comprising a first construct comprising a recognition site for an endonuclease, a first region of homology with a target gene of a microorganism, and a first part of a nucleotide sequence encoding the selection marker, and a second construct comprising a second part of the nucleotide sequence encoding the selection marker, a second region of homology with the target gene of the microorganism, and a copy of the endonuclease recognition site.Type: GrantFiled: April 13, 2015Date of Patent: May 28, 2019Assignee: HEINEKEN SUPPLY CHAIN B.V.Inventors: Jean-Marc Georges Daran, Jacobus Thomas Pronk, Gabriele Romagnoli
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Publication number: 20190127745Abstract: The present invention relates to a yeast cell, in particular a recombinant yeast cell, the cell lacking enzymatic activity needed for the NADH-dependent glycerol synthesis or the cell having a reduced enzymatic activity with respect to the NADH-dependent glycerol synthesis compared to its corresponding wild-type yeast cell, the cell comprising one or more heterologous nucleic acid sequences encoding an NAD+-dependent acetylating acetaldehyde dehydrogenase (EC 1.2.1.10) activity. The invention further relates to the use of a cell according to the invention in the preparation of ethanol.Type: ApplicationFiled: May 3, 2018Publication date: May 2, 2019Inventors: Jacobus Thomas PRONK, Antonius Jeroen Adriaan VAN MARIS, Victor Gabriel GUADALUPE MEDINA
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Publication number: 20180291404Abstract: The present invention relates to a eukaryotic cell that is genetically modified comprising one or more heterologous gene encoding: a) D-glucose-6-phosphate dehydrogenase and/or b) 6-phosphogluconate dehydrogenase; and/or c) glucose dehydrogenase, gluconolactonase and gluconate kinase, wherein a), b) and glucose dehydrogenase in c) are NAD+ dependent.Type: ApplicationFiled: October 3, 2016Publication date: October 11, 2018Applicant: DSM IP Assets B.V.Inventors: Ioannis PAPAPETRIDIS, Jacobus THomas PRONK, Antonius Jeroen Adriaan VAN MARIS, Paul KLAASSEN
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Patent number: 10093937Abstract: The invention relates to a recombinant yeast cell, in particular a transgenic yeast cell, functionally expressing one or more recombinant, in particular heterologous, nucleic acid sequences encoding ribulose-1,5-biphosphate carboxylase oxygenase (Rubisco) and phosphoribulokinase (PRK). The invention further relates to the use of carbon dioxide as an electron acceptor in a recombinant chemotrophic micro-organism, in particular a eukaryotic micro-organism.Type: GrantFiled: February 21, 2014Date of Patent: October 9, 2018Assignee: TECHNISCHE UNIVERSITEIT DELFTInventors: Antonius Jeroen Adriaan Van Maris, Jacobus Thomas Pronk, Victor Gabriel Guadalupe Medina, Hendrik Wouter Wisselink
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Publication number: 20180251773Abstract: The invention relates to a recombinant yeast cell, in particular a transgenic yeast cell, functionally expressing one or more recombinant, in particular heterologous, nucleic acid sequences encoding ribulose-1,5-biphosphate carboxylase oxygenase (Rubisco) and phosphoribulokinase (PRK). The invention further relates to the use of carbon dioxide as an electron acceptor in a recombinant chemotrophic micro-organism, in particular a eukaryotic micro-organism.Type: ApplicationFiled: May 15, 2018Publication date: September 6, 2018Inventors: Antonius Jeroen Adriaan Van Maris, Jacobus Thomas Pronk, Victor Gabriel Guadalupe Medina, Hendrik Wouter Wisselink
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Publication number: 20170321242Abstract: The present invention relates to host cells transformed with a nucleic acid sequence encoding a eukaryotic xylose isomerase obtainable from an anaerobic fungus. When expressed, the sequence encoding the xylose isomerase confers to the host cell the ability to convert xylose to xylulose which may be further metabolized by the host cell. Thus, the host cell is capable of growth on xylose as carbon source. The host cell preferably is a eukaryotic microorganism such as a yeast or a filamentous fungus. The invention further relates to processes for the production of fermentation products such as ethanol, in which a host cell of the invention uses xylose for growth and for the production of the fermentation product. The invention further relates to nucleic acid sequences encoding eukaryotic xylose isomerases and xylulose kinases as obtainable from anaerobic fungi.Type: ApplicationFiled: July 21, 2017Publication date: November 9, 2017Inventors: Hubertus Johannes Marie OP DEN CAMP, Harry Ramanoedj Harhangi, Christiaan Van Der Drift, Jacobus Thomas Pronk
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Publication number: 20170298368Abstract: The invention relates to a nucleic acid molecule encoding a novel selection marker. Said marker is a guanidinobutyrase from Kluyveromyces lactis, which, when expressed in Saccharomyces, allows the growth of the yeast in the presence of guanidinobutyrate as the sole nitrogen source. Said marker can be used in a method for producing a microorganism having an altered genome. The invention further relates to a set of constructs, comprising a first construct comprising a recognition site for an endonuclease, a first region of homology with a target gene of a microorganism, and a first part of a nucleotide sequence encoding the selection marker, and a second construct comprising a second part of the nucleotide sequence encoding the selection marker, a second region of homology with the target gene of the microorganism, and a copy of the endonuclease recognition site.Type: ApplicationFiled: April 13, 2015Publication date: October 19, 2017Inventors: Jean-Marc Georges Daran, Jacobus Thomas Pronk, Gabriele Romagnoli
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Patent number: 9528117Abstract: The present invention relates to a yeast cell, in particular a recombinant yeast cell, the cell lacking enzymatic activity needed for the NADH-dependent glycerol synthesis or the cell having a reduced enzymatic activity with respect to the NADH-dependent glycerol synthesis compared to its corresponding wild-type yeast cell, the cell comprising one or more heterologous nucleic acid sequences encoding an NAD+-dependent acetylating acetaldehyde dehydrogenase (EC 1.2.1.10) activity. The invention further relates to the use of a cell according to the invention in the preparation of ethanol.Type: GrantFiled: February 25, 2014Date of Patent: December 27, 2016Assignee: DSM IP ASSETS B.V.Inventors: Jacobus Thomas Pronk, Antonius Jeroen Adriaan Van Maris, Victor Gabriel Guadalupe Medina
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Publication number: 20160312246Abstract: The present invention relates to a yeast cell, in particular a recombinant yeast cell, the cell lacking enzymatic activity needed for the NADH-dependent glycerol synthesis or the cell having a reduced enzymatic activity with respect to the NADH-dependent glycerol synthesis compared to its corresponding wild-type yeast cell, the cell comprising one or more heterologous nucleic acid sequences encoding an NAD+-dependent acetylating acetaldehyde dehydrogenase (EC 1.2.1.10) activity. The invention further relates to the use of a cell according to the invention in the preparation of ethanol.Type: ApplicationFiled: May 13, 2016Publication date: October 27, 2016Applicant: DSM IP ASSETS B.V.Inventors: Jacobus Thomas PRONK, Antonius Jeroen Adriaan VAN MARIS, Victor Gabriel GUADALUPE MEDINA
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Patent number: 9359613Abstract: The present invention relates to a yeast cell, in particular a recombinant yeast cell, the cell lacking enzymatic activity needed for the NADH-dependent glycerol synthesis or the cell having a reduced enzymatic activity with respect to the NADH-dependent glycerol synthesis compared to its corresponding wild-type yeast cell, the cell comprising one or more heterologous nucleic acid sequences encoding an NAD+-dependent acetylating acetaldehyde dehydrogenase (EC 1.2.1.10) activity. The invention further relates to the use of a cell according to the invention in the preparation of ethanol.Type: GrantFiled: February 25, 2014Date of Patent: June 7, 2016Assignee: DSM IP Assets B.V.Inventors: Jacobus Thomas Pronk, Antonius Jeroen Adriaan Van Maris, Victor Gabriel Guadalupe Medina
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Patent number: 9353376Abstract: The present invention relates to a yeast cell comprising one or more exogenous genes of a pentose metabolic pathway non-native to the yeast cell wherein the yeast cell has a disruption of the hxk1, hxk2 glk1 and gal1 native in the yeast cell. The invention further relates to pentose and glucose fermenting yeast cell that is capable of simultaneous pentose and glucose consumption.Type: GrantFiled: October 11, 2011Date of Patent: May 31, 2016Assignee: DSM IP ASSETS, B.V.Inventors: Hendrik Wouter Wisselink, Antonius Jeroen Adriaan Van Maris, Jacobus Thomas Pronk
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Patent number: 9303253Abstract: The invention relates to an eukaryotic cell expressing nucleotide sequences encoding the ara A, ara B and ara D enzymes whereby the expression of these nucleotide sequences confers on the cell the ability to use L-arabinose and/or convert L-arabinose into L-ribulose, and/or xylulose 5-phosphate and/or into a desired fermentation product such as ethanol. Optionally, the eukaryotic cell is also able to convert xylose into ethanol.Type: GrantFiled: January 6, 2012Date of Patent: April 5, 2016Assignee: DSM IP ASSETS B.V.Inventors: Antonius Jeroen Adriaan Van Maris, Jacobus Thomas Pronk, Hendrik Wouter Wisselink, Johannes Pieter Van Dijken, Aaron Adriaan Winkler, Johannes Hendrik Winde