Abstract: The present invention discloses a novel method for preparing crosslinked biomaterial compositions for use in the augmentation of soft or hard tissue. In general, the method comprises mixing a biocompatible polymer, which is preferably collagen, with a sterile, dry crosslinking agent, which is preferably a synthetic hydrophilic polymer such as a functionally activated polyethylene glycol. Also provided are preferred processes for preparing sterile, dry crosslinking agents contained within syringes for use in the method of the invention. Methods for sterilization of the crosslinking agent include, but are not limited to, sterile filtration, aseptic processing, and e-beam or gamma irradiation. Methods for providing augmentation of soft or hard tissue using crosslinked biomaterial compositions prepared according to the method of the invention are also disclosed.

Abstract: The present invention discloses a novel method for preparing crosslinked biomaterial compositions for use in the augmentation of soft or hard tissue. In general, the method comprises mixing a biocompatible polymer, which is preferably collagen, with a sterile, dry crosslinking agent, which is preferably a synthetic hydrophilic polymer such as a functionally activated polyethylene glycol. Also provided are preferred processes for preparing sterile, dry crosslinking agents contained within syringes for use in the method of the invention. Methods for sterilization of the crosslinking agent include, but are not limited to, sterile filtration, aseptic processing, and e-beam or gamma irradiation. Methods for providing augmentation of soft or hard tissue using crosslinked biomaterial compositions prepared according to the method of the invention am also disclosed.

Abstract: Non-phytotoxic roots coating compositions comprising a polymer, VAM propagules, and a fungicide that selectively inhibits pathogenic fungi are provided. Also provided are methods of coating plant roots with the aforesaid composition and plants comprising roots coated with a polymer containing VAM propagules and a fungicide.In an alternative approach, the roots are first rolled in a dried polymer composition and then dipped in a suspension of VAM propagules, or attached to a film which is a dried polymer containing a spore suspension.

Abstract: Methods and compositions for effecting plant root infection with microorganisms, especially vesicular arbuscular mycorrhizal (VAM) fungi are disclosed. The method relies on a root baiting concept whereby the roots of a plant are caused to grow towards and/or through a material which contains an inoculum of the microorganism. Typically bead-like materials are produced which are comprised of (1) a bait material for which roots have a natural affinity; (2) an inoculum of a microorganism which can infect plant roots and improve their growth and/or health; and (3) a carrier material that supports and localizes the bait and the inoculum. The bait may be water or another suitable material such as a plant growth regulator and the carrier is typically a non-phytotoxic crosslinked polymer especially a water absorbing polymer. The compositions are placed in the vicinity of the plant roots. The bait draws the roots towards the composition to permit infection of the roots with the microorganism.