Patents by Inventor Jacqueline Sharon
Jacqueline Sharon has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 9163232Abstract: The invention relates to a method for manufacturing a recombinant polyclonal protein composition, in particular a recombinant polyclonal antibody composition. The method comprises obtaining a collection of cells transfected with a library of variant nucleic acid sequences, wherein each call in the collection is transfected with and capable of expressing one member of the library, which encodes a distinct member of a polyclonal protein that binds a particular antigen and which is located at the same single site in the genome of individual cells in said collection, wherein said nucleic acid sequence is not naturally associated with said cell in the collection. The cells are cultured under suitable conditions for expression of the polyclonal protein, which is obtained from the cells or culture supernatant. The present method is suitable for manufacturing recombinant polyclonal antibodies, thereby making available a superior replacement of plasma-derived therapeutic immunoglobulin products.Type: GrantFiled: December 19, 2008Date of Patent: October 20, 2015Assignee: Symphogen A/SInventors: John S. Haurum, Finn C. Wiberg, Vincent W. Coljee, Jacqueline Sharon, Chiou-Ying Yang
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Publication number: 20090136498Abstract: The invention relates to a method for manufacturing a recombinant polyclonal protein composition, in particular a recombinant polyclonal antibody composition. The method comprises obtaining a collection of cells transfected with a library of variant nucleic acid sequences, wherein each call in the collection is transfected with and capable of expressing one member of the library, which encodes a distinct member of a polyclonal protein that binds a particular antigen and which is located at the same single site in the genome of individual cells in said collection, wherein said nucleic acid sequence is not naturally associated with said cell in the collection. The cells are cultured under suitable conditions for expression of the polyclonal protein, which is obtained from the cells or culture supernatant. The nucleic acid sequence id introduced into the cells by transfection with a library of vectors for site-specific integration.Type: ApplicationFiled: December 19, 2008Publication date: May 28, 2009Inventors: John S. Haurum, Finn C. Wiberg, Vincent W. Coljee, Jacqueline Sharon, Chiou-Ying Yang
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Publication number: 20060275766Abstract: The invention relates to a method for manufacturing a recombinant polyclonal protein composition, in particular a recombinant polyclonal antibody composition. The method comprises obtaining a collection of cells transfected with a library of variant nucleic acid sequences, wherein each cell in the collection is transfected with and capable of expressing one member of the library, which encodes a distinct member of a polyclonal protein that binds a particular antigen and which is located at the same single site in the genome of individual cells in said collection, wherein said nucleic acid sequence is not naturally associated with said cell in the collection. The cells are cultured under suitable conditions for expression of the polyclonal protein, which is obtained from the cells or culture supernatant. The nucleic acid sequence id introduced into the cells by transfection with a library of vectors for site-specific integration.Type: ApplicationFiled: January 7, 2004Publication date: December 7, 2006Inventors: John Haurum, Finn Wiberg, Vincent Coljee, Jacqueline Sharon, Chiou-Ying Yang
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Publication number: 20060078561Abstract: The invention is directed to methods for the creation and use of libraries of proteins which comprise polyclonal antibodies to a common antigen or group of antigens, receptor proteins with related variable regions, or other immune related proteins with variable regions. These polyclonal antibody libraries can be used to treat or prevent diseases and disorders including neoplasia such as cancer and other malignancies, parasitic infections, bacterial infections, viral infections and disorders such as genetic defects and deficiencies. Protein libraries may be patient-specific, disease-specific or both patient- and disease-specific. Libraries can also be used to detect a disease or disorder in a patient either by direct imaging or through the use of a diagnostic kit. The invention further includes novel cloning methods for the creation and transfer of nucleic acid sequences encoding protein variable regions and novel cloning vectors.Type: ApplicationFiled: April 27, 2004Publication date: April 13, 2006Applicant: The Trustees of Boston UniversityInventor: Jacqueline Sharon
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Patent number: 6335163Abstract: The invention is directed to methods for the creation and use of libraries of proteins which comprise polyclonal antibodies to a common antigen or group of antigens, receptor proteins with related variable regions, or other immune related proteins with variable regions. These polyclonal antibody libraries can be used to treat or prevent diseases and disorders including neoplasia such as cancer and other malignancies, parasitic infections, bacterial infections, viral infections and disorders such as genetic defects and deficiencies. Protein libraries may be patient-specific, disease-specific or both patient- and disease-specific. Libraries can also be used to detect a disease or disorder in a patient either by direct imaging or through the use of a diagnostic kit. The invention further includes novel cloning methods for the creation and transfer of nucleic acid sequences encoding protein variable regions and novel cloning vectors.Type: GrantFiled: April 9, 1998Date of Patent: January 1, 2002Assignee: The Trustees of Boston UniversityInventor: Jacqueline Sharon
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Patent number: 5789208Abstract: The invention is directed to methods for the creation and use of libraries of proteins which comprise polyclonal antibodies to a common antigen or group of antigens, receptor proteins with related variable regions, or other immune related proteins with variable regions. These polyclonal antibody libraries can be used to treat or prevent diseases and disorders including neoplasia such as cancer and other malignancies, parasitic infections, bacterial infections, viral infections and disorders such as genetic defects and deficiencies. Protein libraries may be patient-specific, disease-specific or both patient- and disease-specific. Libraries can also be used to detect a disease or disorder in a patient either by direct imaging or through the use of a diagnostic kit. The invention further includes novel cloning methods for the creation and transfer of nucleic acid sequences encoding protein variable regions and novel cloning vectors.Type: GrantFiled: February 19, 1997Date of Patent: August 4, 1998Assignee: The Trustees of Boston UniversityInventor: Jacqueline Sharon
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Patent number: 5169939Abstract: Chimeric antibodies comprised of the variable region of an antibody chain contiguous with a polypeptide other than the constant region of that antibody chain are disclosed. Such chimeric antibodies can be comprised of the variable region of a chain of an immunoglobulin of selected specificity and an extrinsic polypeptide. In addition to the variable region of a chain of an immunoglobulin of selected specifity and an extrinsic polypeptide, the chimeric antibodies can include at least a portion of the constant region of an immunoglobFUNDING SOURCESThe work described herein was supported by grants from the National Institutes of Health, the American Cancer Society and the Damon Runyon--Walter Winchell Cancer Fund.Type: GrantFiled: April 9, 1989Date of Patent: December 8, 1992Assignee: Massachusetts Institute of Technology & Pres. & Fellows of Harvard CollegeInventors: Malcolm L. Gefter, Mark Ptashne, Jacqueline Sharon