Abstract: Disclosed herein are systems and methods for easy and rapid detection of ocular analytes in vitreous humor or aqueous humor. Specifically, exemplified are systems having a sample acquisition device that is in line with an analyte detection device. The system embodiments allow for the easy procurement and testing of samples. In a typical embodiment, the analyte detection assay device includes a housing that has a reaction chamber that is in fluid communication with a sample conduit and which contains reagents that specifically interact with the analyte. The reaction chamber is in fluid communication with a shunt that is in fluid communication with the sample conduit. The sample conduit has a sample conduit valve that is positioned distally to the shunt.

Abstract: Disclosed herein are systems and methods for detecting ocular analytes in vitreous humor or aqueous humor. Specifically exemplified are systems having a sample acquisition device that is inline with an analyte detection device. The system embodiments allow for the easy procurement and testing of samples. In a typical embodiment, the analyte detection device includes a sample staging chamber and a test chamber that comprises reagents that specifically interact with the analyte. The test chamber may include a sample pad, a conjugate pad having at least one conjugate reagent specific to the analyte loaded thereon, an assay platform having a substrate with at least one test region having a test reagent immobilized thereon, the test reagent being specific to the analyte; and an optional absorbent pad.