Abstract: Provided is a digital surface-enhanced Raman scattering (SERS) sensing platform which allows quantitative detection of a substance to be detected reliably and reproducibly with an excellent limit of detection in a large dynamic range, including: a surface-enhanced Raman scattering (SERS) active reagent which includes Raman active particles including a spherical plasmonic metal core, a plasmonic metal shell having a surface unevenness, and a self-assembled monolayer including a Raman reporter positioned between the core and the shell; a Raman spectroscopic detection unit which performs Raman mapping based on a Raman spectrum which is detected by irradiating the active reagent with an excitation light; and a digital signal analysis unit which analyzes a quantitative detection signal of a substance to be detected by a combination of a Raman signal intensity calculated from the Raman spectrum and a digital count calculated from the Raman mapping.

Abstract: Provided is a Raman-active nanoparticle including: a spherical plasmonic metal core; a plasmonic metal shell having surface irregularities; and a self-assembled monolayer which binds to each of the core and the shell, is positioned between the core and the shell, and includes a Raman reporter satisfying the following Chemical Formula 1: NO2—Ar—SH??(Chemical Formula 1) wherein Ar is a (C6-C12) arylene group.