Patents by Inventor Jae-Jong Kim

Jae-Jong Kim has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20230304082
    Abstract: The present invention relates to a DNA polymerase variant that belongs to family A, and to a use thereof. The present invention relates to: a DNA polymerase variant which easily undergoes polymerization when the base at the 3?-end of a primer is complementary to a template, and yet has inhibited polymerization when the base at the 3?-end of the primer is non-complementary to the template, and thus facilitates discrimination between the two cases; a PCR process using the variant; and a PCR kit comprising the variant. The present invention is useful for single nucleotide polymorphism analysis (SNP genotyping), somatic mutation detection, and the like.
    Type: Application
    Filed: May 28, 2021
    Publication date: September 28, 2023
    Inventors: Jae Jong KIM, Si-Kyu LIM, Jeounghyun RYU, Bo Mi LEE, Seulki KIM, Sun Ho CHA
  • Publication number: 20230046513
    Abstract: The present invention relates to a method and a kit for detecting alleles of which the specificity and sensitivity in a DNA polymerase chain reaction (PCR), which is widely used for the detection of minor alleles such as single nucleotide polymorphisms or somatic mutations, are increased. More specifically, the present invention relates to a PCR-based method and kit for single nucleotide polymorphism (SNP) genotyping and somatic mutation detection, the method and kit adding a partially or fully double-stranded oligonucleotide for increasing discrimination to a PCR solution for selective amplification of alleles, so that PCR amplification is not affected when a primer 3? terminal base is complementary (3?-matched) to a template but PCR amplification is strongly inhibited when a 3? terminal base is not complementary (3?-mismatched).
    Type: Application
    Filed: December 11, 2020
    Publication date: February 16, 2023
    Inventors: Jae Jong KIM, Si-Kyu LIM, In Kyung PARK, AYoung KYUNG, Bo Mi LEE, Jeounghyun RYU, Sun Ho CHA,, SeungWoo BAEK
  • Publication number: 20190316188
    Abstract: Disclosed is a method for detecting single nucleotide polymorphism (SNP) using a feature that the 5?-flap endonuclease (FEN) activity of DNA polymerase is inhibited when a probe complementarily binds to the end of a polymerase chain reaction (PCR) product. More specifically, the present invention relates to a novel method wherein it was verified that, when a probe used for a real-time PCR complementarily binds to the end site of a PCR product, the 5?-FEN activity of thermostable DNA polymerase to the probe is inhibited, and thus when such a feature is used to make a design such that an SNP site to be detected is located at the 5?-end site of the probe, the 5?-flap formation is induced according to the allele, thereby allowing effective SNP detection.
    Type: Application
    Filed: July 1, 2019
    Publication date: October 17, 2019
    Inventors: Jae Jong KIM, Sun Ho CHA, Si Kyu LIM
  • Patent number: 10337052
    Abstract: The present invention relates to a method for detecting single nucleotide polymorphism (SNP) using a feature that the 5?-flap endonuclease (FEN) activity of DNA polymerase is inhibited when a probe complementarily binds to the end of a polymerase chain reaction (PCR) product. More specifically, the present invention relates to a novel method wherein it was verified that, when a probe used for a real-time PCR complementarily binds to the end site of a PCR product, the 5?-FEN activity of thermostable DNA polymerase to the probe is inhibited, and thus when such a feature is used to make a design such that an SNP site to be detected is located at the 5?-end site of the probe, the 5?-flap formation is induced according to the allele, thereby allowing effective SNP detection.
    Type: Grant
    Filed: January 8, 2015
    Date of Patent: July 2, 2019
    Assignee: GENOTECH CORP.
    Inventors: Jae Jong Kim, Sun Ho Cha, Si Kyu Lim
  • Publication number: 20190161879
    Abstract: The present invention relates to a method for manufacturing a porous filter for fine spraying and a porous filter manufactured by the method. A nickel-palladium alloy material porous filter manufactured according to the present invention exhibits excellent corrosion resistance and metal ion elution mitigating effects and can adjust the size of drug particles, thereby allowing a drug to arrive at the desired site. In addition, a plating liquid having a particular composition in the present invention allows the manufacture of a porous filter with a desired thickness by effectively lowering the stress of palladium (Pd), and thus, in a fine sprayer for treating a respiratory disease, a microporous filter, which is capable of delivering a drug to the vicinity of alveoli while effectively preventing the elution of metal elements due to a drug, apparatus vibration, and the like, and a fine sprayer, which uses the microporous filter, can be manufactured.
    Type: Application
    Filed: June 23, 2017
    Publication date: May 30, 2019
    Applicant: DONGGUK UNIVERSITY GYEONGJU CAMPUS INDUSTRY ACADEMY COOPERATION FOUNDATION
    Inventors: Ki Chang NAM, Hyo Chul JI, Jae Jong KIM
  • Patent number: 9505779
    Abstract: The present invention relates to novel tacrolimus analogs, a composition for the prevention or treatment of neurological diseases or immune hypersensitivity disorders comprising the same, a method for preventing or treating neurological diseases or immune hypersensitivity disorders comprising administering the analogs to a subject, a method for preparing the analogs using an isolated modified Streptomyces sp. strain wherein the activity of one or more enzymes selected from the group consisting of TcsA, TcsB, TcsC and TcsD is reduced; and the isolated modified Streptomyces sp. strain for prepare the analogs.
    Type: Grant
    Filed: February 22, 2013
    Date of Patent: November 29, 2016
    Assignee: Intron Biotechnology, Inc.
    Inventors: Yeo Joon Yoon, Jae Jong Kim, Si Kyu Lim
  • Publication number: 20160326577
    Abstract: The present invention relates to a method for detecting single nucleotide polymorphism (SNP) using a feature that the 5?-flap endonuclease (FEN) activity of DNA polymerase is inhibited when a probe complementarily binds to the end of a polymerase chain reaction (PCR) product. More specifically, the present invention relates to a novel method wherein it was verified that, when a probe used for a real-time PCR complementarily binds to the end site of a PCR product, the 5?-FEN activity of thermostable DNA polymerase to the probe is inhibited, and thus when such a feature is used to make a design such that an SNP site to be detected is located at the 5?-end site of the probe, the 5?-flap formation is induced according to the allele, thereby allowing effective SNP detection.
    Type: Application
    Filed: January 8, 2015
    Publication date: November 10, 2016
    Applicant: GENOTECH CORP.
    Inventors: Jae Jong KIM, Sun HO CHA, Si Kyu LIM
  • Patent number: 8609368
    Abstract: Provided is a process for purifying a vancomycin wet body, comprising: dissolving a wet body obtained from a microorganism-fermented solution containing vancomycin into a water soluble solvent to a concentration of about 1 to 40 g/L and carrying out reverse osmosis filtration; and carrying out lyophilization of the filtered vancomycin. The process for purifying a vancomycin wet body provides high-purity vancomycin, while avoiding degradation of stability during a drying step.
    Type: Grant
    Filed: November 26, 2009
    Date of Patent: December 17, 2013
    Assignees: Genotech Co., Ltd., Samyang Biopharmaceuticals Corporation
    Inventors: Jin Suk Cho, Jong Won Yoon, Eun Soo Choi, Jeong Min Kim, Ho Joon Choi, Sung Hag Kim, Seong Kyu Kim, Jae Jong Kim
  • Publication number: 20130230559
    Abstract: The present invention relates to novel tacrolimus analogues, a composition for the prevention or treatment of neurological diseases or immune hypersensitivity disorders comprising the same, a method for preventing or treating neurological diseases or immune hypersensitivity disorders comprising administering the analogues to a subject, a method for preparing the analogues using an isolated modified Streptomyces sp. strain wherein the activity of one or more enzymes selected from the group consisting of TcsA, TcsB, TcsC and TcsD is reduced; and the isolated modified Streptomyces sp. strain for prepare the analogues.
    Type: Application
    Filed: February 22, 2013
    Publication date: September 5, 2013
    Applicant: EWHA University - Industry Collaboration Foundatio
    Inventors: Yeo Joon YOON, Jae Jong KIM, Si Kyu LIM
  • Patent number: 8193345
    Abstract: The present invention relates to a method for separating and purifying a lactone compound with an unsaturated alkyl group from a lactone compound with a saturated alkyl group which is an analog thereof. More specifically, the present invention relates to a method for effectively separating a lactone compound with an unsaturated alkyl group such as FK506 at high purity by extraction with a silver ion (Ag+) solution without use of column chromatography.
    Type: Grant
    Filed: March 18, 2011
    Date of Patent: June 5, 2012
    Assignee: Genotech Corp.
    Inventors: Jae Jong Kim, Si Kyu Lim, Mi Ok Lee, Sang Myoun Lim, Bo-mi Lee, Dong Hwan Kim, Keum Soon Lee, Jeoung Hyun Ryu
  • Publication number: 20110275786
    Abstract: Provided is a process for purifying a vancomycin wet body, comprising: dissolving a wet body obtained from a microorganism-fermented solution containing vancomycin into a water soluble solvent to a concentration of about 1 to 40 g/L and carrying out reverse osmosis filtration; and carrying out lyophilization of the filtered vancomycin. The process for purifying a vancomycin wet body provides high-purity vancomycin, while avoiding degradation of stability during a drying step.
    Type: Application
    Filed: November 26, 2009
    Publication date: November 10, 2011
    Inventors: Jin Suk Cho, Jong Won Yoon, Eun Soo Choi, Jeong Min Kim, Ho Joon Choi, Sung Hag Kim, Seong Kyu Kim, Jae Jong Kim
  • Publication number: 20110183385
    Abstract: The present invention relates to a method of fermenting and purifying tricyclo compounds, specifically FK506 and/or FK520, and more particularly, to a method of purifying tricyclo compounds by adding a hydrophobic absorbent resin as a carrier in culturing FK506 and/or FK520 producing bacteria.
    Type: Application
    Filed: May 6, 2008
    Publication date: July 28, 2011
    Inventors: Jae Jong Kim, Si Kyu Lim, Mi Ok Lee, Sang Myoun Lim, Bo-mi Lee, Dong Hwan Kim, Jeoung Hyun, Keum Soon Lee
  • Publication number: 20110172413
    Abstract: The present invention relates to a method for separating and purifying a lactone compound with an unsaturated alkyl group from a lactone compound with a saturated alkyl group which is an analog thereof. More specifically, the present invention relates to a method for effectively separating a lactone compound with an unsaturated alkyl group such as FK506 at high purity by extraction with a silver ion (Ag+) solution without use of column chromatography.
    Type: Application
    Filed: March 18, 2011
    Publication date: July 14, 2011
    Applicant: GENOTECH CO., LTD
    Inventors: Jae Jong Kim, Si Kyu Lim, Mi Ok Lee, Sang Myoun Lim, Bo-mi Lee, Dong Hwan Kim, Keum Soon Lee, Jeoung Hyun Ryu
  • Publication number: 20040033503
    Abstract: The present invention relates to a method for attaching oligonucleotide to a solid support and an oligonucleotide array prepared by said method. In the present invention, an oligonucleotide array is prepared by the steps of: preparing a hydrophobic attachment layer; spotting solution of oligonucleotide in which a hydrophobic group is bonded to one side of its terminal onto the hydrophobic attachment layer; and solidifying the hydrophobic attachment layer.
    Type: Application
    Filed: June 4, 2003
    Publication date: February 19, 2004
    Inventors: Jae-Jong Kim, Sun-Ho Cha
  • Patent number: 6153412
    Abstract: The present invention provides a lyophilized reagent for PCR which is prepared by adding a stabilizing and sedimenting agent to an aqueous reaction mixture and lyophilizing thereof. The lyophilized PCR reagent of the present invention leads to a simplification of multi-step PCR manipulation, an increase of heat stability of the reaction mixture, prevention of carry-over contamination, and improved credibility of experiments. The lyophilized PCR reagent can be applied as a kit for analysis of DNA sequence or for diagnosis of diseases, which guarantee the results of high credibility in a short period of time.
    Type: Grant
    Filed: December 7, 1998
    Date of Patent: November 28, 2000
    Assignee: Bioneer Corporation
    Inventors: Han-Oh Park, Jae-Jong Kim
  • Patent number: 6127122
    Abstract: The present invention provides an improved method for silver staining of DNA which comprises the steps of linking DNA to a polymer which has a high affinity for the DNA and binding of a plenty of silver ions to the DNA-polymer complex. The silver staining method of the present invention can be applied for the detection and sequencing of trace amount of DNA.
    Type: Grant
    Filed: July 21, 1998
    Date of Patent: October 3, 2000
    Assignee: Bioneer Corporation
    Inventors: Han-Oh Park, Jae-Jong Kim, Jae-Hyung You
  • Patent number: 5861251
    Abstract: The present invention provides a process for preparing a ready-to-use reagent for PCR, which comprises the steps of adding the stabilizer, glucitol or glucose, to an aqueous polymerase chain reaction(PCR) mixture containing a reaction buffer, MgCl.sub.2, dNTPs, a DNA polymerase, the water soluble dye, bromophenol blue, xylene cyanole, bromocresol red or cresol red, a primer, and optionally, ddATP, ddCTP, ddGTP, or ddTTP, and then lyophilizing thereof. The ready-to-use PCR reagent of the present invention leads to a simplification of multi-step PCR manipulation, an increase of heat stability of the reaction mixture, prevention of carry-over contamination, and improved credibility of experiments. The ready-to-use PCR reagent can be applied as a kit for analysis of DNA sequence or for diagnosis of diseases.
    Type: Grant
    Filed: October 15, 1996
    Date of Patent: January 19, 1999
    Assignee: Bioneer Corporation
    Inventors: Han-Oh Park, Jae-Jong Kim