Abstract: Proposed are a method and device of determining optimal complex stimuli for tinnitus treatment. The method of determining the optimal complex stimuli for tinnitus treatment includes: identifying one or more sound stimuli selected by a user input from among a plurality of sound stimuli; identifying one or more electrical stimuli selected by the user input from among a plurality of electrical stimuli; determining a first complex stimulus sequence of complex stimuli in which the one or more selected sound stimuli and the one or more selected electrical stimuli are combined; and applying the complex stimuli to a user, wearing a tinnitus treatment device, according to the determined first complex stimulus sequence.

Abstract: A diagnostic method and a classification method for patients with tinnitus, and a method for providing personalized treatment protocols for the patients with the tinnitus are proposed.

Abstract: The present invention relates to a vagus nerve stimulation device for animals, the device comprising an electrode that contacts the animal's skin to transmit electrical stimuli and a power source for generating electrical stimuli transmitted to the electrode. The present invention has an excellent effect of stabilizing the psychological state of an animal by stimulating the vagus nerve from the outside of the animal. For companion animals, the present invention has the effect of eliminating aggression, relieving and stress, reducing separation anxiety. In the case of livestock animals, there are the effects of improving meat quality and increasing milk production by reducing breeding stress even without the aid of drugs or the like. For leisure animals or sports animals, the excellent effects of imparting psychological stability to reduce the risk of accidents due to psychological stability, improving recovery from fatigue, and improving exercise ability can be brought about.

Abstract: The present invention relates to a method for controlling a complex stimulation, which is a method for controlling a complex stimulation device having an electrical stimulation unit including electrodes in contact with human ears and a sound output unit for stimulating hearing by using sound waves, wherein the sound output unit generates sound waves having different frequencies in both ears, and controls the frequency difference between the sound waves applied to both ears on the basis of frequencies of electrical stimulation applied to the electrodes. The present invention has an excellent effect of improving the efficiency of electrical stimulation to the vagus nerve by stimulating the brain with two types of stimulation by adding acoustic stimulation together when stimulating the vagus nerve through the electrodes in contact with the ears.

Abstract: Proposed is a method for controlling vagus nerve stimulation to stimulate the vagus nerves in both ears, in which one or more of intensity (V), a frequency (F), and a pulse width (PW) of electrical stimulation that is applied to both vagus nerves are separately controlled, and the electrical stimulation that is applied to the vagus nerves in both ears is controlled such that a difference of E expressed by the following [Equation 1] becomes 5,000 or more, [Equation 1] E=V2×F×PW. In this case, as units in the equation 1, the intensity is V, the frequency is Hz, and the pulse width is us. There is an excellent effect that not only can improve an effect of vagus nerve stimulation, but also can activate the brain connected to the vagus nerve when stimulating the vagus nerve through electrodes being in contact with ears.

Abstract: The present invention relates to a pharmaceutical composition comprising exosomes within a chorion extract as an active ingredient for promoting osteogenesis and preventing and treating bone diseases and, more specifically, to excellent osteogenesis promotion efficacy of exosomes within a chorion extract prepared by extracting the placental chorion.

Abstract: Proposed is a treatment system using vagus nerve stimulation. The treatment system includes a treatment part including an electrode configured to be worn on at least one of the left and right ears and configured to generate electrical stimulation on the vagus nerve in the auricle, a controller configured to transmit an electrical stimulation signal for generating the electrical stimulation to the treatment part, an input part configured to allow a user to input user information, and a measurement part configured to measure biometric information on the user. The controller adjusts an intensity and a pattern of the electrical stimulation on the basis of the biometric information measured by the measurement part, and adjusts a treatment protocol including treatment time and treatment intensity on the basis of the biometric information measured by the measurement part and the user information input by the input part.

Abstract: Proposed is a vagus nerve stimulator having at least two electrodes that are configured to be worn on at least one of the left and right ears and are configured to generate electrical stimulation on the vagus nerve in the auricle. The vagus nerve stimulator may include: a body part configured to be located on a side of a wearer's ear; a first electrode configured to be inserted into and make contact with the external acoustic meatus of the wearer's ear; a second electrode configured to make contact with the cymba concha of the wearer's ear; a first electrode fixing part protruding from the body part and having a first electrode at an end thereof; and a second electrode fixing part protruding from the body part and having a second electrode at an end thereof.

Abstract: Proposed is a close-contact type nerve stimulator. The nerve stimulator includes a fixed part which is disposed across the front and rear of an ear, a body part which is rotatably connected to the fixed part, an electrode part which is provided in the body part and is configured to be brought into contact with the auricle or external auditory meatus of the ear, and a control part which controls the angle of rotation of the body part with respect to the fixed part, wherein the electrode part may be brought into close contact with the surface of the auricle or external auditory meatus by the rotation of the body part so as to stimulate the vagus nerve distributed in the auricle or external auditory meatus.

Abstract: A complex stimulation device includes a contact part composed of multiple layers to come into close contact with an auricle or an external auditory meatus, electric stimulation part inserted and distributed with predetermined gaps inside an outer surface of the contact part, a sound output part inserted through a center of the contact part, and a deforming part formed in the contact part.

Abstract: A nerve stimulator according to one embodiment comprises: a pad member including a first pad portion arranged so as to come into contact with the auricle, and a second pad portion arranged so as to come in contact with the external auditory meatus; and a plurality of electrode members provided in the pad member so as to output electro-stimulation, wherein the plurality of electrode members can comprise: a first electrode member provided in the first pad portion so as to come into contact with the auricle; and a second electrode member provided in the second pad portion so as to come into contact with the external auditory meatus.

Abstract: Proposed are a method and device of determining optimal complex stimuli for tinnitus treatment. The method of determining the optimal complex stimuli for tinnitus treatment includes: identifying one or more sound stimuli selected by a user input from among a plurality of sound stimuli; identifying one or more electrical stimuli selected by the user input from among a plurality of electrical stimuli; determining a first complex stimulus sequence of complex stimuli in which the one or more selected sound stimuli and the one or more selected electrical stimuli are combined; and applying the complex stimuli to a user, wearing a tinnitus treatment device, according to the determined first complex stimulus sequence.

Abstract: A nerve stimulator according to one embodiment comprises: a pad member including a first pad portion arranged so as to come into contact with the auricle, and a second pad portion arranged so as to come in contact with the external auditory meatus; and a plurality of electrode members provided in the pad member so as to output electro-stimulation, wherein the plurality of electrode members can comprise: a first electrode member provided in the first pad portion so as to come into contact with the auricle; and a second electrode member provided in the second pad portion so as to come into contact with the external auditory meatus.

Abstract: The present invention relates to a composition for preventing or treating bone diseases, comprising a BMP-2-encoding gene and HSV-tk-encoding gene, and as an active ingredient a stem cell into which a dual kill switch expression vector in which an HGPRT-encoded gene is knocked out is introduced or a cell differentiated from the stem cell, wherein the bone regeneration effect is realized by a BMP-2 growth factor and at the same time, apoptosis may also be dually controlled by the dual kill switch.

Abstract: The present application relates to a method for detecting ligand using a biosensor applied the FRET (fluorescence resonance energy transfer) phenomenon. More particularly, the method may be used for simply detecting a ligand in a sample by measuring the FRET of a biosensor under the conditions in which a specific critical temperature is maintained. The method may use a phenomenon in which a ligand-binding protein in a biosensor shows reversible unfolding at a temperature higher than the specific critical temperature and the level of the unfolding changes depending on the concentration of a ligand. The method can be widely applied to a variety of kinds of FRET biosensors using the ligand-binding protein.

Abstract: A method of detecting and quantifying various enzymatic activities using a constructed artificial genetic circuit GESS (genetic enzyme screening system) for sensing phenolic compounds and a method of screening a trace of activities of target enzymes from a metagenome using the artificial genetic circuit, thereby securing target enzyme genes. When the method for screening and quantifying target enzymatic activity is used, useful genes can be screened from various genetic communities, including environmental or metagenomic libraries, at a single cell level in high throughput (million/day). Further, the sensitivity of the genetic circuit to phenol derivatives and the expression thereof can be controlled, and thus the genetic circuit can rapidly sense and quantify various enzymatic activities. Thus, the method can be advantageously used in the protein engineering technology for enzyme modification.

Abstract: The present invention relates to a method for detecting protein-protein interactions in living cells, and more particularly, to a method for providing cells comprising a first construct and a second construct, wherein the first construct comprises a polynucleotide encoding a first fusion protein which comprises a bait protein, a first fluorescent protein and a CBD (cellulose-binding domain) protein, and wherein the second construct comprises a polynucleotide encoding a second fusion protein which comprises a prey protein and a second fluorescent protein so as to allow formation of inclusion bodies, and detecting interactions between the bait protein and the prey protein that are displayed by inclusion bodies, a method for isolating the prey protein bound to the bait protein using the cells comprising the constructs, the cells, and a kit for detecting protein-protein interactions, comprising the constructs.

Abstract: The present invention relates to a metagenome-derived alkaline phosphatase, and more particularly to a novel, metagenome-derived alkaline phosphatase screened using a artificial genetic circuit that detects phenolic compounds, and a preparation method thereof. A novel alkaline phosphatase according to the present invention has high activity of dephosphorylating DNA and can be inactivated rapidly by simple heat treatment. Thus, it can be used for a dephosphorylation reaction so that genetic manipulations, including genetic cloning, become efficient. In addition, it can be actively expressed in recombinant microorganisms, and thus can be used in various assays, including enzyme immunoassay.

Abstract: The present invention relates to a method for detecting protein-protein interactions in living cells, and more particularly, to a method for providing cells comprising a first construct and a second construct, wherein the first construct comprises a polynucleotide encoding a first fusion protein which comprises a bait protein, a first fluorescent protein and a CBD (cellulose-binding domain) protein, and wherein the second construct comprises a polynucleotide encoding a second fusion protein which comprises a prey protein and a second fluorescent protein so as to allow formation of inclusion bodies, and detecting interactions between the bait protein and the prey protein that are displayed by inclusion bodies, a method for isolating the prey protein bound to the bait protein using the cells comprising the constructs, the cells, and a kit for detecting protein-protein interactions, comprising the constructs.

Abstract: The present invention relates to a metagenome-derived alkaline phosphatase, and more particularly to a novel, metagenome-derived alkaline phosphatase screened using a artificial genetic circuit that detects phenolic compounds, and a preparation method thereof. A novel alkaline phosphatase according to the present invention has high activity of dephosphorylating DNA and can be inactivated rapidly by simple heat treatment. Thus, it can be used for a dephosphorylation reaction so that genetic manipulations, including genetic cloning, become efficient. In addition, it can be actively expressed in recombinant microorganisms, and thus can be used in various assays, including enzyme immunoassay.