Patents by Inventor Jae Kyu Lim
Jae Kyu Lim has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20220213463Abstract: The present invention relates to an Fe—S fusion protein acting as an electron transport chain, a novel carbon monoxide:formate oxidoreductase (CFOR) including the Fe—S fusion protein, novel Thermococcus strain BCF12 transformed with CFOR, and the use thereof. According to the present invention, two different enzymes may be physically linked directly to each other through the Fe—S fusion protein of the present invention, and thus electrons generated from any one enzyme may be transported directly to another enzyme through the Fe—S cluster of the Fe—S fusion protein. Accordingly, a reaction that produces a target substance with high efficiency by directly supplying electrons necessary for the production of the target substance is possible without leakage of electrons generated in any one enzyme. In addition, the present invention has an advantage in that the overall enzyme reaction rate and yield can be dramatically improved using a new electron transport reaction.Type: ApplicationFiled: November 28, 2018Publication date: July 7, 2022Inventors: Jung-Hyun LEE, Jae Kyu LIM, Hyun Sook LEE, Sung Gyun KANG, Kae Kyoung KWON, Yun Jae KIM, Ji-in YANG
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Patent number: 9976124Abstract: The present invention relates to novel hydrogenases isolated from novel hyperthermophilic strains belonging to Thermococcus spp., genes encoding the hydrogenases, and methods of producing hydrogen using strains having the genes. According to the hydrogen production methods of the invention, a large amount of hydrogen can be produced merely by culturing the strains in specific culture conditions. Thus, the methods of the invention have advantages in that they are more economic and efficient than existing hydrogen production methods and can produce hydrogen even at high temperature.Type: GrantFiled: November 29, 2013Date of Patent: May 22, 2018Assignee: KOREA OCEAN RESEARCH & DEVELOPMENT INSTITUTEInventors: Jung Hyun Lee, Sung Gyun Kang, Hyun Sook Lee, Sang Jin Kim, Kae Kyoung Kwon, Sun Shin Cha, Jung Ho Jeon, Yona Cho, Yun Jae Kim, Seung Seop Bae, Jae Kyu Lim, In Soon Jeong
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Patent number: 9267115Abstract: The present invention relates to novel hydrogenases isolated from novel hyperthermophilic strains belonging to Thermococcus spp., genes encoding the hydrogenases, and methods of producing hydrogen using strains having the genes. According to the hydrogen production methods of the invention, a large amount of hydrogen can be produced merely by culturing the strains in specific culture conditions. Thus, the methods of the invention have advantages in that they are more economic and efficient than existing hydrogen production methods and can produce hydrogen even at high temperature.Type: GrantFiled: November 29, 2013Date of Patent: February 23, 2016Assignee: KOREA OCEAN RESEARCH & DEVELOPMENT INSTITUTEInventors: Jung Hyun Lee, Sung Gyun Kang, Hyun Sook Lee, Sang Jin Kim, Kae Kyoung Kwon, Sun Shin Cha, Jung Ho Jeon, Yona Cho, Yun Jae Kim, Seung Seop Bae, Jae Kyu Lim, In Soon Jeong
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Publication number: 20140248683Abstract: The present invention relates to novel hydrogenases isolated from novel hyperthermophilic strains belonging to Thermococcus spp., genes encoding the hydrogenases, and methods of producing hydrogen using strains having the genes. According to the hydrogen production methods of the invention, a large amount of hydrogen can be produced merely by culturing the strains in specific culture conditions. Thus, the methods of the invention have advantages in that they are more economic and efficient than existing hydrogen production methods and can produce hydrogen even at high temperature.Type: ApplicationFiled: November 29, 2013Publication date: September 4, 2014Applicant: KOREA OCEAN RESEARCH & DEVELOPMENT INSTITUTEInventors: Jung Hyun LEE, Sung Gyun KANG, Hyun Sook LEE, Sang Jin KIM, Kae Kyoung KWON, Sun Shin CHA, Jung Ho JEON, Yona CHO, Yun Jae KIM, Seung Seop BAE, Jae Kyu LIM, In Soon JEONG
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Publication number: 20140178960Abstract: The present invention relates to novel hydrogenases isolated from novel hyperthermophilic strains belonging to Thermococcus spp., genes encoding the hydrogenases, and methods of producing hydrogen using strains having the genes. According to the hydrogen production methods of the invention, a large amount of hydrogen can be produced merely by culturing the strains in specific culture conditions. Thus, the methods of the invention have advantages in that they are more economic and efficient than existing hydrogen production methods and can produce hydrogen even at high temperature.Type: ApplicationFiled: November 29, 2013Publication date: June 26, 2014Applicant: KOREA OCEAN RESEARCH & DEVELOPMENT INSTITUTEInventors: Jung Hyun LEE, Sung Gyun KANG, Hyun Sook LEE, Sang Jin KIM, Kae Kyoung KWON, Sun Shin CHA, Jung Ho JEON, Yona CHO, Yun Jae KIM, Seung Seop BAE, Jae Kyu LIM, In Soon JEONG
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Patent number: 8597926Abstract: The present invention relates to novel hydrogenases isolated from novel hyperthermophilic strains belonging to Thermococcus spp., genes encoding the hydrogenases, and methods of producing hydrogen using strains having the genes. According to the hydrogen production methods of the invention, a large amount of hydrogen can be produced merely by culturing the strains in specific culture conditions. Thus, the methods of the invention have advantages in that they are more economic and efficient than existing hydrogen production methods and can produce hydrogen even at high temperature.Type: GrantFiled: September 7, 2009Date of Patent: December 3, 2013Assignee: Korea Ocean Research & Development InstituteInventors: Jung Hyun Lee, Sung Gyun Kang, Hyun Sook Lee, Sang Jin Kim, Kae Kyoung Kwon, Sun Shin Cha, Jung Ho Jeon, Yona Cho, Yun Jae Kim, Seung Seop Bae, Jae Kyu Lim, In Soon Jeong
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Patent number: 8257953Abstract: The present invention relates to a hyperthermophilic DNA polymerase and a preparation method thereof. The invention provides a novel hyperthermophilic DNA polymerase isolated from a Thermococcus sp. strain, a functional equivalent thereof, a protein having the amino acid sequence thereof, and a preparation method thereof. The DNA polymerase according to the invention is a DNA polymerase, which is hyperthermophilic and has an elongation ability and fidelity higher than those of prior commercial DNA polymerases. Thus, the DNA polymerase according to the invention will be useful in precision analysis, precision diagnosis, identification and the like, which require accurate PCR.Type: GrantFiled: October 2, 2006Date of Patent: September 4, 2012Assignee: Korean Ocean Research & Development InstituteInventors: Jung Hyun Lee, Suk Tae Kwon, Sung Gyun Kang, Sang Jin Kim, Jung Ho Hyun, Kae Kyoung Kwon, Yun Jae Kim, Hyun Sook Lee, Seung Seob Bae, Ki Hoon Nam, Jae Kyu Lim, Jung Ho Jeon, Sung Hyun Yang
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Publication number: 20110020896Abstract: The present invention relates to mutant DNA polymerases, their genes and their uses. More specifically, the present invention relates to mutant DNA polymerases which are originally isolated from Thermococcus sp NA1. strain and produced by site-specific mutagenesis, their amino acid sequences, genes encoding said mutant DNA polymerases, their nucleic acids and PCR methods by using thereof. As mutant DNA polymerases according to the present invention have decreased proofreading activity and changed function of inosine sensing effectively compared to wild type DNA polymerase, PCR using primers with specific nucleic acids has made rapid progress. Therefore, the present invention is broadly applicable for PCR in various molecular genetic technologies.Type: ApplicationFiled: October 2, 2007Publication date: January 27, 2011Inventors: Jung Hyun Lee, Sung Gyun Kang, Sang Jin Kim, Kae Kyoung Kwon, Hyun Sook Lee, Yun Jae Kim, Seung Seob Bae, Jae Kyu Lim, Jung Ho Jeon, Yo Na Cho, Suk Tae Kwon
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Publication number: 20100311142Abstract: The present invention relates to novel hydrogenases isolated from novel hyperthermophilic strains belonging to Thermococcus spp., genes encoding the hydrogenases, and methods of producing hydrogen using strains having the genes. According to the hydrogen production methods of the invention, a large amount of hydrogen can be produced merely by culturing the strains in specific culture conditions. Thus, the methods of the invention have advantages in that they are more economic and efficient than existing hydrogen production methods and can produce hydrogen even at high temperature.Type: ApplicationFiled: September 7, 2009Publication date: December 9, 2010Applicant: KOREA OCEAN RESEARCH & DEVELOPMENT INSTITUTEInventors: Jung Hyun Lee, Sung Gyun Kang, Hyun Sook Lee, Sang Jin Kim, Kae Kyoung Kwon, Sun Shin Cha, Jung Ho Jeon, Yona Cho, Yun Jae Kim, Seung Seop Bae, Jae Kyu Lim, In Soon Jeong
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Publication number: 20100297706Abstract: The present invention relates to mutant DNA polymerases and their genes isolated from Thermococcus sp. More specifically, the present invention relates to mutant DNA polymerases which are originally isolated from Thermococcus sp NA1. strain and produced by site-specific mutagenesis, their amino acid sequences, genes encoding said mutant DNA polymerases, their nucleic acids sequences, recombinant vectors containing said nucleic acids sequences, host cells transformed with thereof and methods for producing mutant DNA polymerase protein by using thereof. As mutant DNA polymerases according to the present invention have increased processivity by site-specific mutagenesis on exonuclease active site compared to wild type DNA polymerase, the present invention is broadly applicable for PCR in various molecular genetic technologies.Type: ApplicationFiled: October 2, 2007Publication date: November 25, 2010Applicant: KOREA OCEAN RESEARCH & DEVELOPMENT INSTITUTEInventors: Jung Hyun Lee, Sung Gyun Kang, Sang Jin Kim, Kae Kyoung Kwon, Hyun Sook Lee, Yun Jae Kim, Yong Gu Ryu, Seung Seob Bae, Jae Kyu Lim, Jung Ho Jeon, Yo Na Cho
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Publication number: 20090148896Abstract: The present invention relates to a hyperthermophilic DNA polymerase and a preparation method thereof. The invention provides a novel hyperthermophilic DNA polymerase isolated from a Thermococcus sp. strain, a functional equivalent thereof, a protein having the amino acid sequence thereof, and a preparation method thereof. The DNA polymerase according to the invention is a DNA polymerase, which is hyperthermophilic and has an elongation ability and fidelity higher than those of prior commercial DNA polymerases. Thus, the DNA polymerase according to the invention will be useful in precision analysis, precision diagnosis, identification and the like, which require accurate PCR.Type: ApplicationFiled: October 2, 2006Publication date: June 11, 2009Applicant: Korea Ocean Research & Development InstituteInventors: Jung Hyun Lee, Suk Tae Kwon, Sung Gyun Kang, Sang Jin Kim, Jung Ho Hyun, Kae Kyoung Kwon, Yun Jae Kim, Hyun Sook Lee, Seung Seob Bae, Ki Hoon Nam, Jae Kyu Lim, Jung Ho Jeon, Sung Hyun Yang