Abstract: Antibody-mediated rejection (ABMR) is one of the main obstacles to successful transplantation, including ABO blood group-incompatible (ABOi) transplantation. C4d deposition is a marker of ABMR and is also found in most ABOi allograft tissues. Described herein are anti-C4d CAR Tregs that suppress ABMR in ABOi allografts. Anti-C4d CAR Tregs prepared by retroviral transduction of CAR into CD62L +CD4 +CD25 +Tregs, expressed Foxp3, CD25, CTLA-4, LAP, and GITR to similar extents as non-transduced Tregs. Anti-C4d CAR Tregs were activated by specific binding to C4d and suppressed in vitro T cell proliferation as well as non-transduced Tregs. Furthermore, adoptive transfer of anti-C4d CAR Tregs significantly prolonged mouse ABOi heart allograft survival (P<0.05).

Abstract: A method of maintaining a temperature of an aftertreatment system of a vehicle, the method including: operating, by a controller, a retarder reducing driving force of a propeller shaft of the vehicle in response to a retarder operation request signal; operating, by the controller, a jake brake device which discharges a fuel-air mixture compressed in an explosion stroke of the engine to an exhaust pipe and decreases revolutions per minute (RPM) of the engine or an exhaust brake device which blocks a discharge of the exhaust gas of the engine to a rear end of the exhaust pipe and decreases the RPM of the engine, in order to remove the output error value; and controlling, by the controller, the engine so that an amount of exhaust gas introduced into the aftertreatment system is decreased.

Abstract: A method of maintaining a temperature of an aftertreatment system of a vehicle, the method including: operating, by a controller, a retarder reducing driving force of a propeller shaft of the vehicle in response to a retarder operation request signal; operating, by the controller, a jake brake device which discharges a fuel-air mixture compressed in an explosion stroke of the engine to an exhaust pipe and decreases revolutions per minute (RPM) of the engine or an exhaust brake device which blocks a discharge of the exhaust gas of the engine to a rear end of the exhaust pipe and decreases the RPM of the engine, in order to remove the output error value; and controlling, by the controller, the engine so that an amount of exhaust gas introduced into the aftertreatment system is decreased.

Abstract: A leak detection method of a reducing agent pumping system including a reducing agent tank, an injector, a line formed through the reducing agent tank and the injector, and a pumping module disposed in the line may include pumping a reducing agent to the injector with a pump of the pumping module and detecting a pressure thereof, operating the pump at a predetermined load or less and stopping an operation of the injector, when it is determined that a detected pressure is between a first set value and a second set value, operating a heater for heating the reducing agent in the pumping module for a predetermined time, and determining that leak occurred in the line between the pumping module and the injector, when it is determined a temperature of the reducing agent in the pumping module is lower than a predetermined value.

Abstract: A leak detection method of a reducing agent pumping system including a reducing agent tank, an injector, a line formed through the reducing agent tank and the injector, and a pumping module disposed in the line may include pumping a reducing agent to the injector with a pump of the pumping module and detecting a pressure thererof, operating the pump at a predetermined load or less and stopping an operation of the injector, when it is determined that a detected pressure is between a first set value and a second set value, operating a heater for heating the reducing agent in the pumping module for a predetermined time, and determining that leak occurred in the line between the pumping module and the injector, when it is determined a temperature of the reducing agent in the pumping module is lower than a predetermined value.

Abstract: In a method for preventing clogging of a urea injection nozzle in an after-run operation of a selective catalytic reduction (SCR) system which supplies urea stored in a urea tank through a supply module, a urea injection nozzle and a urea line connecting the supply module and the urea injection nozzle, the method may include an emptying step where the urea is withdrawn into the urea tank by creating negative pressure in the supply module and a pressure equilibrium step where the pressure in the supply module is recovered by stopping creation of negative pressure in the supply module, wherein in the pressure equilibrium step, whether to forcibly inject the urea is controlled on the basis of pressure after a predetermined time has passed since the pressure equilibrium step began.

Abstract: In a method for preventing clogging of a urea injection nozzle in an after-run operation of a selective catalytic reduction (SCR) system which supplies urea stored in a urea tank through a supply module, a urea injection nozzle and a urea line connecting the supply module and the urea injection nozzle, the method may include an emptying step where the urea is withdrawn into the urea tank by creating negative pressure in the supply module and a pressure equilibrium step where the pressure in the supply module is recovered by stopping creation of negative pressure in the supply module, wherein in the pressure equilibrium step, whether to forcibly inject the urea is controlled on the basis of pressure after a predetermined time has passed since the pressure equilibrium step began.

Abstract: A method for preventing choking of a urea injection nozzle in after run operation on a SCR system which supplies urea stored in a urea tank through a supply module, a urea injection nozzle and a urea line connecting the supply module and the urea injection nozzle, the method for preventing choking of a urea injection nozzle includes comparing pressure within the supply module with a first predetermined pressure (Pa), open controlling the urea injection nozzle when the pressure in the supply module is higher than the first predetermined pressure (Pa) or repeating open/close controlling the urea injection nozzle when the pressure in the supply module is lower than the first predetermined pressure (Pa), pressure equilibrium which recovers pressure in the supply module and stopping the after run operation when the balancing pressure is completed.

Abstract: A method for preventing choking of a urea injection nozzle in after run operation on a SCR system which supplies urea stored in a urea tank through a supply module, a urea injection nozzle and a urea line connecting the supply module and the urea injection nozzle, the method for preventing choking of a urea injection nozzle includes comparing pressure within the supply module with a first predetermined pressure (Pa), open controlling the urea injection nozzle when the pressure in the supply module is higher than the first predetermined pressure (Pa) or repeating open/close controlling the urea injection nozzle when the pressure in the supply module is lower than the first predetermined pressure (Pa), pressure equilibrium which recovers pressure in the supply module and stopping the after run operation when the balancing pressure is completed.