Abstract: A method of treating a subject with Hutchinson-Gilford progeria syndrome includes administering to the subject a composition which includes an allogeneic haploidentical adipose tissue-derived stromal vascular fraction (SVF). The an allogeneic haploidentical adipose tissue-derived SVF may be produced by a process including centrifuging an allogeneic haploidentical adipose tissue lipoaspirate to obtain a packed adipose tissue, mixing the packed adipose tissue with collagenase, mincing the packed adipose tissue mixed with the collagenase by using a homogenizer, incubating the minced adipose tissue, centrifuging the incubated adipose tissue to separate and remove the collagenase, and repeating the centrifuging to obtain the stromal vascular fraction.

Abstract: A composition for treating Hutchinson-Gilford progeria syndrome according to an embodiment of the present invention includes a stromal vascular fraction (SVF). The stromal vascular fraction (SVF) may be produced by a process including centrifuging an allogeneic haploidentical adipose tissue lipoaspirate to obtain a packed adipose tissue, mixing the packed adipose tissue with collagenase, mincing the packed adipose tissue mixed with the collagenase by using a homogenizer, incubating the minced adipose tissue, centrifuging the incubated adipose tissue to separate and remove the collagenase, and repeating the centrifuging to obtain the stromal vascular fraction.

Abstract: The present invention provides pharmaceutical compositions, methods of preparations, and uses for treatments, preventions or alleviation of bone and cartilage diseases. The present invention discloses methods and pharmaceutical compositions comprising adipose tissue-derived stem cells, platelet rich plasma, calcium chloride, and hyaluronic acid for the treatments, preventions, or alleviation of bone diseases; methods and a pharmaceutical compositions further comprising of dexamethasone for treatments, preventions, or alleviation of cartilage diseases.

Abstract: The present invention provides pharmaceutical compositions, and methods of preparation and use for the treatment, prevention or alleviation of bone and cartilage diseases. The present invention discloses a method and a pharmaceutical composition comprising adipose-derived stem cells, platelet rich plasma, calcium chloride, and hyaluronic acid for the treatment, prevention, or alleviation of bone diseases, and a method and a pharmaceutical composition further comprising dexamethasone for the treatment, prevention, or alleviation of cartilage diseases.