Abstract: A transfection reagent composition comprising: 30-60 MOL. % of a cationic lipid, or a pharmaceutical acceptable salt thereof; 10-60 MOL % of a structural lipid; 10-20 MOL % of a triglyceride; and 0.1 to about 10 MOL. % of a stabilizing agent, is provided. The transfection agent is effective in transfecting cells, particularly neurons, with siRNA, mRNA and plasmid nucleic acid, Sand maintaining viability of the cells as well as activity of the delivered nucleic acid.

Abstract: A transfection reagent composition comprising: 30-60 MOL % of an cationic lipid, or pharmaceutical acceptable salt thereof; 10-60 MOL % structural lipid; a sterol and 0.1 to about 10 MOL % of a stabilizing agent is provided. The reagent is particularly adapted for neuron and related cell types. A method of manufacturing LNP including nucleic acid for selective uptake into either neurons or astrocytes or neural progenitor cells is also provided.

Abstract: A transfection reagent composition comprising: 30-60 MOL. % of a cationic lipid, or a pharmaceutical acceptable salt thereof; 10-60 MOL % of a structural lipid; 10-20 MOL % of a triglyceride; and 0.1 to about 10 MOL. % of a stabilizing agent, is provided. The transfection agent is effective in transfecting cells, particularly neurons, with siRNA, mRNA and plasmid nucleic acid, Sand maintaining viability of the cells as well as activity of the delivered nucleic acid.

Abstract: A device for extinguishing fires in a flammable liquid storage tank uses either foam or dry chemical powder, or both, as the extinguishant. The device comprises at least one annular pipe ring arranged in a horizontal plane just above the bottom of the storage tank and connected to a supply of extinguishant. When more than one ring is used, the rings are concentrically and equidistantly arranged with the outermost ring connected to the supply of extinguishant. The pipe rings have two or more diametrically connected cross-members. Vertically extending discharge pipes are situated at the junctions of the pipe rings and cross-members. The top ends of the discharge pipes are above the highest level of flammable liquid in the storage tank and are equipped with discharge nozzles. The discharge nozzles provide for uniform discharge of the extinguishant onto the surface of the flammable liquid held in the storage tank.