Abstract: A modular, small molecule regulated single chain variable fragment (scFv) fusion protein is disclosed. The scFv fusion protein comprises a ligand binding protein fused to a protein that binds to an exogenous control molecule, wherein the scFv fusion protein is directly regulated by the control molecule. Binding of the control molecule to the ligand binding protein induces a change in the affinity of the scFv for a target antigen. Methods of using the fusion protein to treat diseases such as cancer are also described.

Abstract: A modular, small molecule regulated single chain variable fragment (scFv) fusion protein is disclosed. The scFv fusion protein comprises a ligand binding protein fused to a protein that binds to an exogenous control molecule, wherein the scFv fusion protein is directly regulated by the control molecule. Binding of the control molecule to the ligand binding protein induces a change in the affinity of the scFv for a target antigen. Methods of using the fusion protein to treat diseases such as cancer are also described.

Abstract: A real-time search system enables askers to identify and submit questions to topically and skill level relevant potential answerers. A computer server receives and analyzes short text questions, determines a corresponding set of informational facets semantically and topically characterizing the question. The informational facets are evaluated against a database index of informational facets identified from prior analyzed messages correlated by profile identifiers of message originators to provide an identification of a plurality of potential answerers. The question is distributed to the plurality of potential answerers and ensuing message conversations between the asker responsive answerers are monitored for quality and sufficiency of response. The stored profiles of responsive answerers are updated to reflect the occurrence and quality of response.