Abstract: Methods and kits are provided for producing sense RNA molecules. The sense RNA molecules can be used in various research and diagnostic applications, such as gene expression studies involving nucleic acid microarrays.

Abstract: Methods and kits are provided for producing sense RNA molecules. The sense RNA molecules are prepared by: providing a single stranded cDNA molecule having 5? and 3? ends; attaching an oligodeoxynucleotide tail to the 3? end of said single stranded cDNA molecule; providing a double stranded RNA polymerase promoter having a sense strand and antisense strand, wherein the sense strand comprises a single stranded 3? overhang comprising a sequence complementary to said oligodeoxynucleotide tail; annealing said double stranded RNA polymerase promoter to said oligodeoxynucleotide tail by complementary base pairing with said 3? overhang sequence; ligating the 5? end of the antisense strand of said double stranded RNA polymerase promoter to the 3? end of said oligodeoxynucleotide tail; and initiating RNA transcription using an RNA polymerase which recognizes said double stranded promoter, thus producing a sense RNA molecule (sRNA).

Abstract: Methods and kits are provided for producing sense RNA molecules. The sense RNA molecules can be used in various research and diagnostic applications, such as gene expression studies involving nucleic acid microarrays.