Patents by Inventor Jaiprakash Shewale
Jaiprakash Shewale has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 11820978Abstract: The present teachings relate to the extraction of nucleic acid from solid materials. Provided are useful compositions, methods, and kits for obtaining nucleic acids from a solid biological sample or an adhesive material having a biological material adherent or embedded within the adhesive substrate. The extracted nucleic acid can be used in downstream applications such as genotyping, detection, quantification, and identification of the source of the biological material.Type: GrantFiled: January 11, 2021Date of Patent: November 21, 2023Assignee: LIFE TECHNOLOGIES CORPORATIONInventors: James Stray, Jason Yingjie Liu, Maxim Brevnov, Jaiprakash Shewale, Allison Holt
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Publication number: 20210198655Abstract: The present teachings relate to the extraction of nucleic acid from solid materials. Provided are useful compositions, methods, and kits for obtaining nucleic acids from a solid biological sample or an adhesive material having a biological material adherent or embedded within the adhesive substrate. The extracted nucleic acid can be used in downstream applications such as genotyping, detection, quantification, and identification of the source of the biological material.Type: ApplicationFiled: January 11, 2021Publication date: July 1, 2021Inventors: James STRAY, Jason Yingjie Liu, Maxim Brevnov, Jaiprakash Shewale, Allison Holt
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Patent number: 10894957Abstract: The present teachings relate to the extraction of nucleic acid from solid materials. Provided are useful compositions, methods and kits for obtaining nucleic acids from a solid biological sample or an adhesive material having a biological material adherent or embedded within the adhesive substrate. The extracted nucleic acid can be used in downstream applications such as genotyping, detection, quantification, and identification of the source of the biological material.Type: GrantFiled: June 21, 2018Date of Patent: January 19, 2021Assignee: Life Technologies CorporationInventors: James Stray, Jason Yingjie Liu, Maxim Brevnov, Jaiprakash Shewale, Allison Holt
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Publication number: 20180371449Abstract: The present teachings relate to the extraction of nucleic acid from solid materials. Provided are useful compositions, methods and kits for obtaining nucleic acids from a solid biological sample or an adhesive material having a biological material adherent or embedded within the adhesive substrate. The extracted nucleic acid can be used in downstream applications such as genotyping, detection, quantification, and identification of the source of the biological material.Type: ApplicationFiled: June 21, 2018Publication date: December 27, 2018Inventors: James STRAY, Jason Yingjie Liu, Maxim Brevnov, Jaiprakash Shewale, Allison Holt
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Publication number: 20160348092Abstract: The present teachings relate to the extraction of nucleic acid from solid materials. Provided are useful compositions, methods and kits for obtaining nucleic acids from a solid biological sample or an adhesive material having a biological material adherent or embedded within the adhesive substrate. The extracted nucleic acid can be used in downstream applications such as genotyping, detection, quantification, and identification of the source of the biological material.Type: ApplicationFiled: August 12, 2016Publication date: December 1, 2016Inventors: James STRAY, Jason Yingjie Liu, Maxim Brevnov, Jaiprakash Shewale, Allison Holt
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Publication number: 20160326515Abstract: Methods and materials are disclosed for use in recovering a biopolymer from a solution. In particular, the invention provides methods for extraction and isolation of nucleic acids from biological materials. The nucleic acids can be separated by forming a stable complex with soluble polysaccharide polymers and magnetic particles, in the presence of detergents and solvent. When the particles are magnetically separated out of the solution, the nucleic acids are separated with them. The nucleic acids can subsequently be released and separated from the particles. The nucleic acid preparation is useful for achieving efficient and accurate results in downstream molecular techniques such as quantification, identification of the source of the nucleic acids, and genotyping.Type: ApplicationFiled: July 21, 2016Publication date: November 10, 2016Inventors: Maxim BREVNOV, Hemant PAWAR, Manohar FURTADO, Jaiprakash SHEWALE
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Patent number: 9481909Abstract: The invention relates to a method for simultaneous quantification of human nuclear DNA and human male DNA in a biological sample while also detecting the presence of PCR inhibitors in a single reaction. The multiplex quantification method also provides a ratio of human nuclear and male DNA present in a biological sample. Such sample characterization is useful for achieving efficient and accurate results in downstream molecular techniques such as genotyping.Type: GrantFiled: June 19, 2015Date of Patent: November 1, 2016Assignee: APPLIED BIOSYSTEMS, LLCInventors: Jaiprakash Shewale, Manohar Furtado, Pius Brzoska, Maura Barbisin, Rixun Fang, Michael Malicdem, Cristin O'Shea
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Patent number: 9447409Abstract: The present teachings relate to the extraction of nucleic acid from solid materials. Provided are useful compositions, methods and kits for obtaining nucleic acids from a solid biological sample or an adhesive material having a biological material adherent or embedded within the adhesive substrate. The extracted nucleic acid can be used in downstream applications such as genotyping, detection, quantification, and identification of the source of the biological material.Type: GrantFiled: September 14, 2010Date of Patent: September 20, 2016Assignee: LIFE TECHNOLOGIES CORPORATIONInventors: James Stray, Jason Yingjie Liu, Maxim Brevnov, Jaiprakash Shewale, Allison Holt
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Patent number: 9422544Abstract: Methods and materials are disclosed for use in recovering a biopolymer from a solution. In particular, the invention provides methods for extraction and isolation of nucleic acids from biological materials. The nucleic acids can be separated by forming a stable complex with soluble polysaccharide polymers and magnetic particles, in the presence of detergents and solvent. When the particles are magnetically separated out of the solution, the nucleic acids are separated with them. The nucleic acids can subsequently be released and separated from the particles. The nucleic acid preparation is useful for achieving efficient and accurate results in downstream molecular techniques such as quantification, identification of the source of the nucleic acids, and genotyping.Type: GrantFiled: June 28, 2013Date of Patent: August 23, 2016Assignee: LIFE TECHNOLOGIES CORPORATIONInventors: Maxim Brevnov, Hemant Pawar, Manohar Furtado, Jaiprakash Shewale
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Publication number: 20150361493Abstract: The invention relates to a method for simultaneous quantification of human nuclear DNA and human male DNA in a biological sample while also detecting the presence of PCR inhibitors in a single reaction. The multiplex quantification method also provides a ratio of human nuclear and male DNA present in a biological sample. Such sample characterization is useful for achieving efficient and accurate results in downstream molecular techniques such as genotyping.Type: ApplicationFiled: June 19, 2015Publication date: December 17, 2015Inventors: Jaiprakash SHEWALE, Manohar Furtado, Pius Brzoska, Maura Barbisin, Rixun Fang, Michael Malicdem, Cristin O'Shea
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Publication number: 20140248626Abstract: In general, the disclosed method can be used to remove contaminating microbes and nucleic acids from microorganisms-derived reagents, apparatus and processes (materials and apparatus) related to PCR (and RT-PCR), including sample prep reagents and materials that are used to isolate, purify and detect nucleic acids.Type: ApplicationFiled: February 27, 2014Publication date: September 4, 2014Applicant: LIFE TECHNOLOGIES CORPORATIONInventors: Jason Yingjie LIU, Elena Bolchakova, Jaiprakash Shewale, Manohar Furtado
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Publication number: 20140039176Abstract: Methods and materials are disclosed for use in recovering a biopolymer from a solution. In particular, the invention provides methods for extraction and isolation of nucleic acids from biological materials. The nucleic acids can be separated by forming a stable complex with soluble polysaccharide polymers and magnetic particles, in the presence of detergents and solvent. When the particles are magnetically separated out of the solution, the nucleic acids are separated with them. The nucleic acids can subsequently be released and separated from the particles. The nucleic acid preparation is useful for achieving efficient and accurate results in downstream molecular techniques such as quantification, identification of the source of the nucleic acids, and genotyping.Type: ApplicationFiled: June 28, 2013Publication date: February 6, 2014Inventors: Maxim BREVNOV, Hemant PAWAR, Manohar FURTADO, Jaiprakash SHEWALE
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Patent number: 8497065Abstract: Methods and materials are disclosed for use in recovering a biopolymer from a solution. In particular, the invention provides methods for extraction and isolation of nucleic acids from biological materials. The nucleic acids can be separated by forming a stable complex with soluble polysaccharide polymers and magnetic particles, in the presence of detergents and solvent. When the particles are magnetically separated out of the solution, the nucleic acids are separated with them. The nucleic acids can subsequently be released and separated from the particles. The nucleic acid preparation is useful for achieving efficient and accurate results in downstream molecular techniques such as quantification, identification of the source of the nucleic acids, and genotyping.Type: GrantFiled: February 13, 2009Date of Patent: July 30, 2013Assignee: Life Technologies CorporationInventors: Maxim G. Brevnov, Hemant S. Pawar, Manohar R. Furtado, Jaiprakash Shewale
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Patent number: 8445657Abstract: The invention relates to a method for simultaneous quantification of human nuclear DNA and human male DNA in a biological sample while also detecting the presence of PCR inhibitors in a single reaction. The multiplex quantification method also provides a ratio of human nuclear and male DNA present in a biological sample. Such sample characterization is useful for achieving efficient and accurate results in downstream molecular techniques such as genotyping.Type: GrantFiled: August 26, 2011Date of Patent: May 21, 2013Assignee: Applied Biosystems, LLCInventors: Jaiprakash Shewale, Manohar Furtado, Pius Brzoska, Maura Barbisin, Rixun Fang, Michael Malicdem, Cristin O'Shea
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Publication number: 20110195486Abstract: In general, the disclosed method can be used to remove contaminating microbes and nucleic acids from microorganisms-derived reagents, apparatus and processes (materials and apparatus) related to PCR (and RT-PCR), including sample prep reagents and materials that are used to isolate, purify and detect nucleic acids.Type: ApplicationFiled: May 22, 2009Publication date: August 11, 2011Applicant: LIFE TECHNOLOGIES CORPORATIONInventors: Yingjie Liu, Elena Bolchakova, Jaiprakash Shewale, Manohar Furtado
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Publication number: 20110159485Abstract: The present teachings relate to the extraction of nucleic acid from solid materials. Provided are useful compositions, methods and kits for obtaining nucleic acids from a solid biological sample or an adhesive material having a biological material adherent or embedded within the adhesive substrate. The extracted nucleic acid can be used in downstream applications such as genotyping, detection, quantification, and identification of the source of the biological material.Type: ApplicationFiled: September 14, 2010Publication date: June 30, 2011Applicant: LIFE TECHNOLOGIES CORPORATIONInventors: James Stray, Jason Yingjie Liu, Maxim Brevnov, Jaiprakash Shewale, Allison Holt
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Publication number: 20100029925Abstract: Methods and materials are disclosed for use in recovering a biopolymer from a solution. In particular, the invention provides methods for extraction and isolation of nucleic acids from biological materials. The nucleic acids can be separated by forming a stable complex with soluble polysaccharide polymers and magnetic particles, in the presence of detergents and solvent. When the particles are magnetically separated out of the solution, the nucleic acids are separated with them. The nucleic acids can subsequently be released and separated from the particles. The nucleic acid preparation is useful for achieving efficient and accurate results in downstream molecular techniques such as quantification, identification of the source of the nucleic acids, and genotyping.Type: ApplicationFiled: May 22, 2009Publication date: February 4, 2010Applicant: LIFE TECHNOLOGIES CORPORATION, a Delaware CorporationInventors: Maxim G. Brevnov, Jaiprakash Shewale, Manohar R. Furtado, Hemant S. Pawar
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Publication number: 20090209751Abstract: Methods and materials are disclosed for use in recovering a biopolymer from a solution. In particular, the invention provides methods for extraction and isolation of nucleic acids from biological materials. The nucleic acids can be separated by forming a stable complex with soluble polysaccharide polymers and magnetic particles, in the presence of detergents and solvent. When the particles are magnetically separated out of the solution, the nucleic acids are separated with them. The nucleic acids can subsequently be released and separated from the particles. The nucleic acid preparation is useful for achieving efficient and accurate results in downstream molecular techniques such as quantification, identification of the source of the nucleic acids, and genotyping.Type: ApplicationFiled: February 13, 2009Publication date: August 20, 2009Applicant: LEFE TECHNOLOGIES CORPORATION, a Delaware CorporationInventors: Maxim G. Brevnov, Hemant S. Pawar, Manohar R. Furtado, Jaiprakash Shewale
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Publication number: 20070003944Abstract: The insertion polymorphisms based on interspersed elements including LINEs and SINEs is used for the inference of an individual's geographic origin. SINE polymorphisms are identical-by-descent, essentially homoplasy-free, and inexpensive to genotype using a variety of approaches. Using a Structure analysis of the Alu insertion polymorphism based genotypes, the geographic affiliation of unknown human individuals can be inferred with high levels of confidence. This technique to infer the geographic affiliation of unknown human DNA samples can be a useful tool in forensic genomics.Type: ApplicationFiled: August 12, 2005Publication date: January 4, 2007Inventors: Sudhir Sinha, Jaiprakash Shewale, Mark Batzer, Jerilyn Walker, David Ray
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Publication number: 20060141512Abstract: The development of an isolation methodology for separation of human sperm cells from biological samples containing human epithelial cells is provided. Using sperm binding proteins, glycopeptides, lectins, derivatives of N-acetylglucosamine, triazine dyes or inhibitors of glycosyltransferase linked to an insoluble support, our invention enables binding of human sperm cells from biological samples. Bound sperms can be dissociated and used for in vitro analyses or subsequently lysed on the insoluble support for isolation of male specific DNA. Other cell types such as epithelial cells, white blood cells and cell debris present in the biological samples are not bound to the derivatized insoluble support. The sperm cells, thus isolated, can be processed for isolation of nuclear DNA for human identification and forensic DNA analysis.Type: ApplicationFiled: December 2, 2005Publication date: June 29, 2006Inventors: Sudhir Sinha, Jaiprakash Shewale