Abstract: A delivery system for implants used in structural heart diseases by a minimally invasive method comprises a catheter (2) which is connected with the distal section of the balloon (1) and on the other side with the straight cap with the lateral canal (10) to which a pressure syringe for pumping the balloon is attached. The catheter is covered from the outside with a special shield (8). The balloon (1) has a necking in the middle section and inside the balloon and in the catheter (8) there is a canal (6) led outside the balloon formed at the end in the shape of a coil (3). Inside the balloon (1) or outside it at its distal and proximal ends the system is equipped with at least one anchoring mechanism shapely formed crimped on the balloon surface of the implant preventing its uncontrolled moving.

Abstract: A low profile balloon expandable artificial prosthetic heart valve, particularly aortic, for transcatheter implantation comprises a valve frame of cylindrical structure. The cuff made of the polymer material is inseparably integrated with the frame so that the valve frame in the supporting section is embedded or sewn into and covered from the internal side and from the outside with the cuff material, which folded into the interior section of the frame and in the frame valve section is formed into valve leaflets in bicuspid or tricuspid configuration and in the said section it is attached to the frame and to the frame posts, and the cuff material excess is led into the longitudinal openings in the posts.

Abstract: A method of forming prefabricated units used in production of systems of prosthetic aortic valve transcatheter implantation and prosthetic aortic valve prefabricated unit with an non-thrombogenic smooth surface layer or with a porous fibrous layer constituting a scaffold for epithelium cell culture, intended for manufacturing TAVI system. Stents for covering and solutions of polycarbonate silicones and/or polycarbonate urethanes and/or polyurethane with average molecular weight in the range from 50 000 g/mol to 200 000 g/mol in the solvent DMAC are prepared. Initially a smooth layer of polycarbonate silicone is applied in the electrospinning machine by electrospraying with use of the solution in DMAC with the concentration of 2-8% w/w.

Abstract: The present invention relates to a genetically encoded FRET-based biosensor to monitor the activity of matrix metalloproteinase 9 (MMP-9). MMP-9 is an extracellular acting endopeptidase implicated in both physiological and pathological processes. A genetically encoded FRET biosensor anchored in the cellular membrane allows studying the proteolytic activity of MMP-9 with high spatiotemporal resolution at the exact region of MMP-9 action on the cell. Applicability of the biosensor, both in vitro and in vivo in living cells, has been demonstrated by ratiometric analysis of cleavage of the biosensor by a purified auto-activating mutant of MMP-9.

Abstract: The present invention relates to a genetically encoded FRET-based biosensor to monitor the activity of matrix metalloproteinase 9 (MMP-9). MMP-9 is an extracellular acting endopeptidase implicated in both physiological and pathological processes. A genetically encoded FRET biosensor anchored in the cellular membrane allows studying the proteolytic activity of MMP-9 with high spatiotemporal resolution at the exact region of MMP-9 action on the cell. Applicability of the biosensor, both in vitro and in vivo in living cells, has been demonstrated by ratiometric analysis of cleavage of the biosensor by a purified auto-activating mutant of MMP-9.

Abstract: A computer-implemented method for processing an image comprising dendritic spines, the method comprising the steps of obtaining the image comprising at least one dendritic spine (110), obtaining the coordinates of the tip point (311) and the base point (312), detecting the skeleton (317) of the dendritic spine (110) by analyzing the brightness of consecutive image portions (316) arranged perpendicularly to an axis extending through the tip point (311) and the base point (312) and for each image portion (316) selecting the brightest point distanced not more than a predefined threshold (s) from the brightest point (314) of the previous image portion (316), detecting the contour (319) of the dendritic spine (310) by analyzing the brightness of consecutive image portions (318) arranged perpendicularly to the skeleton (317) and selecting the contour points (320) as points at which the plot brightness of the image portion transits the point having the brightness lower than the brightness (B) of the skeleton point m