Abstract: The embodiments herein provide a nanoparticle system for targeted gene delivery or a drug delivery and a method of synthesizing the same. The nanoparticle composition for targeted gene transfer and drug delivery comprises a protein, a chitosan and a lipid. A method of synthesizing the nanoparticles involves preparing a gelatine and chitosan gel. A milky colloid solution is prepared with the gelatine, chitosan solution and a phosphatidylcholine. The milky colloid is homogenized for the self assembly of the nanoparticles. The milky colloid is subjected to high speed and high pressure homogenizer. The CHO cells are transfected with nanoparticles and lipofectamine 2000 for comparing the transfection efficiency. The nanoparticles deliver DNA, RNA, ribozyme and nucleotide sequences. The nanoparticles deliver lipophylic and hydrophilic drugs. The transfection efficiency of gene and drug is higher when the target cells are transferred with nanoparticles, compared to the cells transferred with lipofectamine 2000.

Abstract: The embodiments herein provide a method of synthesis for metallic nano-products such as metallic nano-antennas, metallic nano-rods, metallic nano-wires, metallic nano-prolate spheroids and metallic nano-oblate spheroids using saturated and unsaturated carboxylic acids. The method involves preparing a reaction mixture by mixing a polar protic solvent, a polar aprotic solvent or a non-polar solvent, a saturated fatty acid or an unsaturated fatty acid and a metallic salt. Then a mixture of reducing agents is prepared by mixing a polar protic solvent, a polar aprotic solvent or a non-polar solvent and a saturated fatty acid or an unsaturated fatty acid. The reaction mixture and the mixture of reducing agents are combined and heated solvo-thermally for a pre-determined period of time at a pre-determined temperature.

Abstract: The various embodiments herein provide an electrospun fiber mat filter comprising a cigarette filter containing macrocycle for removing toxic compounds from a toxic material, wherein the toxic material comprises liquid, gas, and cigarette smoke and a method of synthesizing the same. The electrospun fiber mat cigarette filter comprises a biological, organic or synthetic macrocycle, plurality of additives, a solvent and an acceptable polymeric carrier. The biological macromolecules are engineered polyhemoglobin and/or chlorophyll. The biological, organic, or synthetic macrocycle are electrospun with the acceptable polymeric carrier in presence of an abruptly asymmetric electric field to form an electrospun fiber mat. The electrospun fiber mat is made up of networks of plurality of nanofibers.

Abstract: The embodiments herein provide a nanoparticle system for targeted gene delivery or a drug delivery and a method of synthesising the same. The nanoparticle composition for targeted gene transfer and drug delivery comprises a protein, a chitosan and a lipid. A method of synthesizing the nanoparticles involves preparing a gelatine and chitosan gel. A milky colloid solution is prepared with the gelatine, chitosan solution and a phosphatidylcholine. The milky colloid is homogenized for the self assembly of the nanoparticles. The milky colloid is subjected to high speed and high pressure homogenizer. The CHO cells are transfected with nanoparticles and lipofectamine 2000 for comparing the transfection efficiency. The nanoparticles deliver DNA, RNA, ribozyme and nucleotide sequences. The nanoparticles deliver lipophylic and hydrophilic drugs. The transfection efficiency of gene and drug is higher when the target cells are transferred with nanoparticles, compared to the cells transferred with lipofectamine 2000.

Abstract: The various embodiments herein relate to an injectable matrix used for regeneration, reconstruction, repair or replacement of organ or tissue. The injectable matrix consists of a synthetic and natural polymer, a stem cell niche and nanoparticles in the form of cups filled with growth factor and physiologic agent. The embodiments herein also provide a method for regeneration, reconstruction, repair or replacement of organ or tissue. In the method, an injectable matrix is injected to create three dimensional matrix system or network in an area of the desired tissue or organ, migration of blood circulatory stem cells or tissue-specific progenitor cells occur to the injected area of the tissue or organ. The growth factors and physiological agent present in the nanocups are released. The stem cells proliferate and differentiate to form the desired organ or tissue.

Abstract: The various embodiments herein provide an electrospun fiber mat filter comprising a cigarette filter containing macrocycle for removing toxic compounds from a toxic material, wherein the toxic material comprises liquid, gas, and cigarette smoke and a method of synthesizing the same. The electrospun fiber mat cigarette filter comprises a biological, organic or synthetic macrocycle, plurality of additives, a solvent and an acceptable polymeric carrier. The biological macromolecules are engineered polyhemoglobin and/or chlorophyll. The biological, organic, or synthetic macrocycle are electrospun with the acceptable polymeric carrier in presence of an abruptly asymmetric electric field to form an electrospun fiber mat. The electrospun fiber mat is made up of networks of plurality of nanofibers.

Abstract: The embodiments herein provide a method of synthesis for metallic nano-products such as metallic nano-antennas, metallic nano-rods, metallic nano-wires, metallic nano-prolate spheroids and metallic nano-oblate spheroids using saturated and unsaturated carboxylic acids. The method involves preparing a reaction mixture by mixing a polar protic solvent, a polar aprotic solvent or a non-polar solvent, a saturated fatty acid or an unsaturated fatty acid and a metallic salt. Then a mixture of reducing agents is prepared by mixing a polar protic solvent, a polar aprotic solvent or a non-polar solvent and a saturated fatty acid or an unsaturated fatty acid. The reaction mixture and the mixture of reducing agents are combined and heated solvo-thermally for a pre-determined period of time at a pre-determined temperature.

Abstract: The various embodiments herein relate to an injectable matrix used for regeneration, reconstruction, repair or replacement of organ or tissue. The injectable matrix consists of a synthetic and natural polymer, a stem cell niche and nanoparticles in the form of cups filled with growth factor and physiologic agent. The embodiments herein also provide a method for regeneration, reconstruction, repair or replacement of organ or tissue. In the method, an injectable matrix is injected to create three dimensional matrix system or network in an area of the desired tissue or organ, migration of blood circulatory stem cells or tissue-specific progenitor cells occur to the injected area of the tissue or organ. The growth factors and physiological agent present in the nanocups are released. The stem cells proliferate and differentiate to form the desired organ or tissue.