Patents by Inventor James A. Baum
James A. Baum has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20040221334Abstract: Disclosed are novel synthetically-modified B. thuringiensis nucleic acid segments encoding &dgr;-endotoxins having insecticidal activity against lepidopteran insects. Also disclosed are synthetic crystal proteins encoded by these novel nucleic acid sequences. Methods of making and using these genes and proteins are disclosed as well as methods for the recombinant expression, and transformation of suitable host cells. Transformed host cells and transgenic plants expressing the modified endotoxin are also aspects of the invention. Also disclosed are methods for modifying, altering, and mutagenizing specific loop regions between the &agr; helices in domain 1 of these crystal proteins, including Cry1C, to produce genetically-engineered recombinant cry* genes, and the proteins they encode which have improved insecticidal activity. In preferred embodiments, novel Cry1C* amino acid segments and the modified cry1C* nucleic acid sequences which encode them are disclosed.Type: ApplicationFiled: May 27, 2004Publication date: November 4, 2004Applicant: Monsanto Technology LLC.Inventors: James A. Baum, Amy Jelen Gilmer, Anne-Marie Light Mettus
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Patent number: 6809078Abstract: Disclosed are novel synthetically-modified B. thuringiensis nucleic acid segments encoding &dgr;-endotoxins having insecticidal activity against lepidopteran insects. Also disclosed are synthetic crystal proteins encoded by these novel nucleic acid sequences. Methods of making and using these genes and proteins are disclosed as well as methods for the recombinant expression, and transformation of suitable host cells. Transformed host cells and transgenic plants expressing the modified endotoxin are also aspects of the invention. Also disclosed are methods for modifying, altering, and mutagenizing specific loop regions between the &agr; helices in domain 1 of these crystal proteins, including Cry1C, to produce genetically-engineered recombinant cry* genes, and the proteins they encode which have improved insecticidal activity. In preferred embodiments, novel Cry1C* amino acid segments and the modified cry1C* nucleic acid sequences which encode them are disclosed.Type: GrantFiled: October 5, 2001Date of Patent: October 26, 2004Assignee: Monsanto Technology LLCInventors: James A. Baum, Amy Jelen Gilmer, Anne-Marie Light Mettus
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Publication number: 20030237111Abstract: Disclosed are Bacillus thuringiensis strains comprising novel crystal proteins which exhibit insecticidal activity against lepidopteran insects. Also disclosed are novel B. thuringiensis genes and their encoded crystal proteins, as well as methods of making and using transgenic cells comprising the novel nucleic acid sequences of the invention.Type: ApplicationFiled: May 2, 2003Publication date: December 25, 2003Applicant: Monsanto Technology LLC.Inventors: James A. Baum, Chih-Rei Chu, William P. Donovan, Amy J. Gilmer, Mark J. Rupar
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Publication number: 20030232757Abstract: Disclosed are novel insecticidal polypeptides, and compositions comprising these polypeptides, peptide fragments thereof, and antibodies specific therefor. Also disclosed are vectors, transformed host cells, and transgenic plants that contain nucleic acid segments that encode the disclosed &dgr;-endotoxin polypeptides. Also disclosed are methods of identifying related polypeptides and polynucleotides, methods of making and using transgenic cells comprising these polynucleotide sequences, as well as methods for controlling an insect population, such as Colorado potato beetle, southern corn rootworm and western corn rootworm, and for conferring to a plant resistance to a target insect species.Type: ApplicationFiled: April 7, 2003Publication date: December 18, 2003Applicant: Monsanto Technology LLCInventors: Mark J. Rupar, William P. Donovan, Chih-Rei Chu, Elizabeth Pease, Yuping Tan, Annette C. Slaney, Thomas M. Malvar, James A. Baum
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Publication number: 20030195336Abstract: Disclosed are novel synthetically-modified B. thuringiensis nucleic acid segments encoding &dgr;-endotoxins having insecticidal activity against lepidopteran insects. Also disclosed are synthetic crystal proteins encoded by these novel nucleic acid sequences. Methods of making and using these genes and proteins are disclosed as well as methods for the recombinant expression, and transformation of suitable host cells. Transformed host cells and transgenic plants expressing the modified endotoxin are also aspects of the invention. Also disclosed are methods for modifying, altering, and mutagenizing specific loop regions between the &agr; helices in domain 1 of these crystal proteins, including Cry1C, to produce genetically-engineered recombinant cry* genes, and the proteins they encode which have improved insecticidal activity. In preferred embodiments, novel Cry1C* amino acid segments and the modified cry1C* nucleic acid sequences which encode them are disclosed.Type: ApplicationFiled: July 22, 2002Publication date: October 16, 2003Applicant: Monsanto Technology LLC.Inventors: James A. Baum, Amy Jelen Gilmer, Anne-Marie Light Mettus
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Patent number: 6593293Abstract: Disclosed are Bacillus thuringiensis strains comprising novel crystal proteins which exhibit insecticidal activity against lepidopteran insects. Also disclosed are novel B. thuringiensis genes and their encoded crystal proteins, as well as methods of making and using transgenic cells comprising the novel nucleic acid sequences of the invention.Type: GrantFiled: September 13, 2000Date of Patent: July 15, 2003Assignee: Monsanto Technology, LLCInventors: James A. Baum, Chih-Rei Chu, William P. Donovan, Amy J. Gilmer, Mark J. Rupar
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Publication number: 20030101482Abstract: Disclosed are novel synthetically-modified B. thuringiensis nucleic acid segments encoding &dgr;-endotoxins having insecticidal activity against lepidopteran insects. Also disclosed are synthetic crystal proteins encoded by these novel nucleic acid sequences. Methods of making and using these genes and proteins are disclosed as well as methods for the recombinant expression, and transformation of suitable host cells. Transformed host cells and transgenic plants expressing the modified endotoxin are also aspects of the invention. Also disclosed are methods for modifying, altering, and mutagenizing specific loop regions between the &agr; helices in domain 1 of these crystal proteins, including Cry1C, to produce genetically-engineered recombinant cry* genes, and the proteins they encode which have improved insecticidal activity. In preferred embodiments, novel Cry1C* amino acid segments and the modified cry1C* nucleic acid sequences which encode them are disclosed.Type: ApplicationFiled: October 5, 2001Publication date: May 29, 2003Applicant: Monsanto Technology LLCInventors: James A. Baum, Amy Jelen Gilmer, Anne-Marie Light Mettus
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Patent number: 6555655Abstract: Disclosed are novel insecticidal polypeptides, and compositions comprising these polypeptides, peptide fragments thereof, and antibodies specific therefor. Also disclosed are vectors, transformed host cells, and transgenic plants that contain nucleic acid segments that encode the disclosed &dgr;-endotoxin polypeptides. Also disclosed are methods of identifying related polypeptides and polynucleotides, methods of making and using transgenic cells comprising these polynucleotide sequences, as well as methods for controlling an insect population, such as Colorado potato beetle, southern corn rootworm and western corn rootworm, and for conferring to a plant resistance to a target insect species.Type: GrantFiled: May 3, 2000Date of Patent: April 29, 2003Assignee: Monsanto Technology, LLCInventors: Mark J. Rupar, William P. Donovan, Chih-Rei Chu, Elizabeth Pease, Yuping Tan, Annette C. Slaney, Thomas M. Malvar, James A. Baum
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Publication number: 20030068335Abstract: Disclosed is a novel Lepidopteran- and Coleopteran-active &dgr;-endotoxin polypeptide, and compositions comprising the polypeptide, peptide fragments thereof, and antibodies specific therefor. Also disclosed are vectors, transformed host cells, and transgenic plants that comprise nucleic acid segments encoding the polypeptide. Also disclosed are methods of identifying related polypeptides and polynucleotides, methods of making and using transgenic cells comprising the novel sequences of the invention, as well as methods for controlling an insect population, such as the Western Corn Rootworm and Colorado potato beetle, and for conferring to a plant population resistance to the target insect species.Type: ApplicationFiled: April 10, 2002Publication date: April 10, 2003Applicant: Monsanto Technology LLC.Inventors: Anne-Marie Light Mettus, James A. Baum
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Patent number: 6482636Abstract: The present invention provides constructed Bacillus thuringiensis (B.t.) strains that are toxic to mosquito larvae. The mosquito-toxic B.t. strains have improved characteristics, such as increased production of mosquito-toxic proteins, reduced number of spores or increased variety of mosquito-toxic proteins, compared with the well-known, mosquito-toxic strain Bacillus thuringiensis subspecies israelensis (B.t. israelensis). The present invention involves insertion of an antibiotic-resistance gene into the B.t. israelensis mosquito-toxin plasmid having a size of approximately 75 mDa such that the plasmid is “tagged” with antibiotic resistance. The advantage of the tagged mosquito-toxin plasmid is that it permits detection of the rare event in which the mosquito-toxin plasmid is transferred from the B.t. israelensis strain (the donor strain) into a non-B.t. israelensis strain (the recipient strain) that normally does not harbor the mosquito-toxin plasmid. Non-B.t.Type: GrantFiled: August 12, 1999Date of Patent: November 19, 2002Assignee: Certis USA, L.L.C.Inventors: William P. Donovan, James A. Baum
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Patent number: 6468523Abstract: Disclosed is a novel Lepidopteran- and Coleopteran-active &dgr;-endotoxin polypeptide, and compositions comprising the polypeptide, peptide fragments thereof, and antibodies specific therefor. Also disclosed are vectors, transformed host cells, and transgenic plants that comprise nucleic acid segments encoding the polypeptide. Also disclosed are methods of identifying related polypeptides and polynucleotides, methods of making and using transgenic cells comprising the novel sequences of the invention, as well as methods for controlling an insect population, such as the Western Corn Rootworm and Colorado potato beetle, and for conferring to a plant population resistance to the target insect species.Type: GrantFiled: November 2, 1998Date of Patent: October 22, 2002Assignee: Monsanto Technology LLCInventors: Anne-Marie Light Mettus, James A. Baum
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Patent number: 6423828Abstract: Disclosed are novel synthetically-modified B. thuringiensis nucleic acid segments encoding &dgr;-endotoxins having insecticidal activity against lepidopteran insects. Also disclosed are synthetic crystal proteins encoded by these novel nucleic acid sequences. Methods of making and using these genes and proteins are disclosed as well as methods for the recombinant expression, and transformation of suitable host cells. Transformed host cells and transgenic plants expressing the modified endotoxin are also aspects of the invention. Also disclosed are methods for modifying, altering, and mutagenizing specific loop regions between the &agr; helices in domain 1 of these crystal proteins, including Cry1C, to produce genetically-engineered recombinant cry* genes, and the proteins they encode which have improved insecticidal activity. In preferred embodiments, novel Cry1C* amino acid segments and the modified cry1C* nucleic acid sequences which encode them are disclosed.Type: GrantFiled: June 22, 1999Date of Patent: July 23, 2002Assignee: Monsanto Technology LLCInventors: James A. Baum, Amy Jelen Gilmer, Anne-Marie Light Mettus
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Patent number: 6313378Abstract: Disclosed are novel synthetically-modified B. thuringiensis nucleic acid segments encoding &dgr;-endotoxins having insecticidal activity against lepidopteran insects. Also disclosed are synthetic crystal proteins encoded by these novel nucleic acid sequences. Methods of making and using these genes and proteins are disclosed as well as methods for the recombinant expression, and transformation of suitable host cells. Transformed host cells and transgenic plants expressing the modified endotoxin are also aspects of the invention. Also disclosed are methods for modifying, altering, and mutagenizing specific loop regions between the &agr; helices in domain 1 of these crystal proteins, including Cry1C, to produce genetically-engineered recombinant cry* genes, and the proteins they encode which have improved insecticidal activity. In preferred embodiments, novel Cry1C* amino acid segments and the modified cry1C* nucleic acid sequences which encode them are disclosed.Type: GrantFiled: June 21, 1999Date of Patent: November 6, 2001Assignee: Monsanto Technology LLCInventors: James A. Baum, Amy Jelen Gilmer, Anne-Marie Light Mettus
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Lepidopteran-toxic polypeptide and polynucleotide compositions and methods for making and using same
Patent number: 6177615Abstract: Disclosed are novel synthetically-modified B. thuringiensis nucleic acid segments encoding &dgr;-endotoxins having insecticidal activity against lepidopteran insects. Also disclosed are synthetic crystal proteins encoded by these novel nucleic acid sequences. Methods of making and using these genes and proteins are disclosed as well as methods for the recombinant expression, and transformation of suitable host cells. Transformed host cells and transgenic plants expressing the modified endotoxin are also aspects of the invention. Also disclosed are methods for modifying, altering, and mutagenizing specific loop regions between the &agr; helices in domain 1 of these crystal proteins, including Cry1C, to produce genetically-engineered recombinant cry* genes, and the proteins they encode which have improved insecticidal activity. In preferred embodiments, novel Cry1C* amino acid segments and the modified cry1C* nucleic acid sequences which encode them are disclosed.Type: GrantFiled: February 17, 1999Date of Patent: January 23, 2001Assignee: Monsanto CompanyInventor: James A. Baum -
Patent number: 6153814Abstract: Disclosed are novel synthetically-modified B. thuringiensis nucleic acid segments encoding .delta.-endotoxins having insecticidal activity against lepidopteran insects. Also disclosed are synthetic crystal proteins encoded by these novel nucleic acid sequences. Methods of making and using these genes and proteins are disclosed as well as methods for the recombinant expression, and transformation of suitable host cells. Transformed host cells and transgenic plants expressing the modified endotoxin are also aspects of the invention. Also disclosed are methods for modifying, altering, and mutagenizing specific loop regions between the .alpha. helices in domain 1 of these crystal proteins, including Cry1C, to produce genetically-engineered recombinant cry* genes, and the proteins they encode which have improved insecticidal activity. In preferred embodiments, novel Cry1C* amino acid segments and the modified cry1C* nucleic acid sequences which encode them are disclosed.Type: GrantFiled: February 17, 1999Date of Patent: November 28, 2000Assignee: Monsanto CompanyInventors: James A. Baum, Amy Jelen Gilmer, Anne-Marie Light Mettus
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Patent number: 6033874Abstract: Disclosed are novel synthetically-modified B. thuringiensis nucleic acid segments encoding .delta.-endotoxins having insecticidal activity against lepidopteran insects. Also disclosed are synthetic crystal proteins encoded by these novel nucleic acid sequences. Methods of making and using these genes and proteins are disclosed as well as methods for the recombinant expression, and transformation of suitable host cells. Transformed host cells and transgenic plants expressing the modified endotoxin are also aspects of the invention. Also disclosed are methods for modifying, altering, and mutagenizing specific loop regions between the .alpha. helices in domain 1 of these crystal proteins, including Cry1C, to produce genetically-engineered recombinant cry* genes, and the proteins they encode which have improved insecticidal activity. In preferred embodiments, novel Cry1C* amino acid segments and the modified cry1C* nucleic acid sequences which encode them are disclosed.Type: GrantFiled: May 18, 1999Date of Patent: March 7, 2000Assignee: Ecogen, Inc.Inventors: James A. Baum, Amy Jelen Gilmer, Ann-Marie Light Mettus
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Patent number: 5965428Abstract: The present invention is directed to a chimeric crystal protein comprising CryIF and CryIAc. Bacillus thuringiensis isolates comprising said protein and a method of controlling lepidopterans are also included in the present invention.Type: GrantFiled: October 8, 1996Date of Patent: October 12, 1999Assignee: Ecogen, Inc.Inventors: Amy Jelen Gilmer, James A. Baum
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Patent number: 5942664Abstract: Disclosed are novel synthetically-modified B. thuringiensis nucleic acid segments encoding .delta.-endotoxins having insecticidal activity against lepidopteran insects. Also disclosed are synthetic crystal proteins encoded by these novel nucleic acid sequences. Methods of making and using these genes and proteins are disclosed as well as methods for the recombinant expression, and transformation of suitable host cells. Transformed host cells and transgenic plants expressing the modified endotoxin are also aspects of the invention. Also disclosed are methods for modifying, altering, and mutagenizing specific loop regions between the .alpha. helices in domain 1 of these crystal proteins, including Cry1C, to produce genetically-engineered recombinant cry* genes, and the proteins they encode which have improved insecticidal activity. In preferred embodiments, novel Cry1C* amino acid segments and the modified cry1C* nucleic acid sequences which encode them are disclosed.Type: GrantFiled: November 27, 1996Date of Patent: August 24, 1999Assignee: Ecogen, Inc.Inventors: James A. Baum, Amy Jelen Gilmer, Anne-Marie Light Mettus
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Patent number: 5914318Abstract: Disclosed are novel synthetically-modified B. thuringiensis nucleic acid segments encoding .delta.-endotoxins having insecticidal activity against lepidopteran insects. Also disclosed are synthetic crystal proteins encoded by these novel nucleic acid sequences. Methods of making and using these genes and proteins are disclosed as well as methods for the recombinant expression, and transformation of suitable host cells. Transformed host cells and transgenic plants expressing the modified endotoxin are also aspects of the invention. Also disclosed are methods for modifying, altering, and mutagenizing specific loop regions between the .alpha. helices in domain 1 of these crystal proteins, including Cry1C, to produce genetically-engineered recombinant cry* genes, and the proteins they encode which have improved insecticidal activity. In preferred embodiments, novel Cry1C* amino acid segments and the modified cry1C* nucleic acid sequences which encode them are disclosed.Type: GrantFiled: November 26, 1997Date of Patent: June 22, 1999Assignee: Ecogen, Inc.Inventors: James A. Baum, Amy Jelen Gilmer, Anne-Marie Light Mettus
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Patent number: 5843744Abstract: A transposable element, or transposon, isolated from Bacillus thuringiensis (B.t.) and designated as transposon Tn5401. The invention also includes a method of using this transposon in a site-specific recombination system for construction of recombinant B.t. strains that contain insecticidal B.t. toxin protein genes and that are free of DNA not native to B.t.Type: GrantFiled: June 24, 1994Date of Patent: December 1, 1998Assignee: Ecogen Inc.Inventor: James A. Baum