Abstract: Methods of genomic screening to identify epigenetically silenced genes, including epigenetically silenced tumor suppressor genes are provided. Also provided are methods of detecting a cancer, for example, an esophageal squamous cell carcinoma or a head and neck squamous cell carcinoma, as are methods of treating a subject having such a cancer.

Abstract: A shear cutter drill bit for drilling a subterranean formation is provided having a bit body; a bit face on the bit body having at least a center zone, an efficiency zone, and an outer zone, the efficiency zone being located between the center zone and the outer zone; and a plurality of cutters having a backrake angle located on the bit face; wherein a majority of the cutters located in the efficiency zone of the bit face have a more aggressive backrake angle than a majority of the cutters located in the center zone of the bit face and a majority of the cutter located in the outer zone of the bit face.

Abstract: A method of identifying epigenetically silenced genes, e.g., methylation silenced genes, in cancer cells is provided. In addition, methods of identifying a cancer by detecting epigenetic silencing of gene expression are provided, as are methods of treating a subject having such a cancer, for example, a colorectal cancer and/or gastric cancer. Reagents for practicing such methods also are provided.

Abstract: A genome wide microarray gene expression approach for human colorectal cancer cells was used to identify hundreds of hypermethylated genes for colon cancer. We compared isogenic cells altered pharmacologically versus genetically to induce genomic demethylation, to pinpoint genes activated by DNA demethylation, but not by inhibition of class I and II histone deacetylases (HDACs). We achieve an 82% success rate in predicting genes with densely hypermethylated CpG islands and complete gene silencing. The genes are similarly hypermethylated in primary tumors and have previously undetected tumor suppressor functions. The genes can be used diagnostically to detect cancer, pre-cancer, and likelihood of developing cancer.

Abstract: The present invention relates to a tag-modified bisulfite genomic sequencing (tBGS) method developed for simplified evaluation of DNA methylation sites. The method employs direct cycle sequencing of PCR products at kilobase scale, without conventional DNA fragment cloning. The method entails subjecting bisulfite-modified genomic DNA to a second-round PCR amplification employing GC-tagged primers. The invention also relates to a method for identifying a patient at risk for lung cancer using the tBGS technique disclosed.

Abstract: A method of identifying epigenetically silenced genes, e.g., methylation silenced genes, in cancer cells is provided. In addition, methods of identifying a cancer by detecting epigenetic silencing of gene expression are provided, as are methods of treating a subject having such a cancer, for example, a colorectal cancer and/or gastric cancer. Reagents for practicing such methods also are provided.

Abstract: An pneumatic apparatus that attaches to pneumatic sanding tools that when In use provides a directed stream of air that creates a cushion of air between the sand paper and the work surface helping to keep the sand paper, the work surface and the worker clean from dust and debris.

Abstract: The present invention provides methods and systems for net shaped manufacturing using carbon nanotubes. Generally, an automatic control unit is used to place reaction units in the proper location to produce a component part of carbon nanotubes in a predetermined configuration. The reaction units include a carbon vaporization unit, a carbon feed/injection unit and a gas pressure/temperature control isolation unit. The carbon feed/injection unit advantageously operates to inject carbon based materials (e.g., graphite powder, solid graphite or carbon based gas) into an reaction area at a predetermined rate in which the carbon vaporization unit provides energy capable of dissociating carbon atoms from the injected carbon based material to produce a predetermined concentration of carbon vapor within the reaction area. The gas pressure/temperature control isolation unit operates to control the pressure and temperature of the reaction area to promote the growth of carbon nanotubes.

Abstract: A method of applying a label to a container which includes an intermediate portion of the sidewall thereof which has an annular curved surface wherein the vertical height of the portion having a curved surface is a minor portion of the entire height of the container. A narrow rectangular label of shrinkable material is first provided with one or more longitudinally extending strips of adhesive extending intermediate the longitudinal edges of the label at the area of the label which is to contact the portion of greatest diameter on the curved portion of the container. The strip of adhesive material is shorter than the length of the label so that the adhesive material does not contact the overlapping edges of the label. An adhesive is applied to the trailing edge of the label only and the label is wrapped about the compound curved portion with the adhesive strip engaging the curved portion of the container at the area of greatest diameter.

Abstract: The present invention provides methods and systems for net shaped manufacturing using carbon nanotubes. Generally, an automatic control unit is used to place reaction units in the proper location to produce a component part of carbon nanotubes in a predetermined configuration. The reaction units include a carbon vaporization unit, a carbon feed/injection unit and a gas pressure/temperature control isolation unit. The carbon feed/injection unit advantageously operates to inject carbon based materials (e.g., graphite powder, solid graphite or carbon based gas) into an reaction area at a predetermined rate in which the carbon vaporization unit provides energy capable of dissociating carbon atoms from the injected carbon based material to produce a predetermined concentration of carbon vapor within the reaction area. The gas pressure/temperature control isolation unit operates to control the pressure and temperature of the reaction area to promote the growth of carbon nanotubes.

Abstract: A method of applying a label to a container which includes an intermediate portion of the side wall thereof which has an annular curved surface wherein the vertical height of the portion having a curved surface is a minor portion of the entire height of the container. A narrow rectangular label of shrinkable material is first provided with one or more longitudinally extending strips of adhesive extending intermediate the longitudinal edges of the label at the area of the label which is to contact the portion of greatest diameter on the curved portion of the container. The strip of adhesive material is shorter than the length of the label so that the adhesive material does not contact the overlapping edges of the label. An adhesive is applied to the trailing edge of the label only and the label is wrapped about the compound curved portion with the adhesive strip engaging the curved portion of the container at the area of greatest diameter.

Abstract: A method of and an apparatus for conditionally bypassing the error correction function of a large scale integrated (LSI) semiconductor random access memory (RAM) is disclosed. A content addressable memory (CAM) is utilized to store the addresses of the addressable locations in the RAM in which an error was previously detected, and on each memory reference both the CAM and the RAM are simultaneously referenced by the same address. Upon a memory reference, the read data from, i.e., the date read out of, the RAM is concurrently coupled directly to an Interface Register and directly to the error detection and correction circuitry (ECC) and thence to the Interface Register. If the CAM does not contain the address, the read data that is coupled to the Interface Register is gated out at a first relatively early gate pulse. However, if the CAM does contain the address, the corrected read data from the ECC is then gated out of the Interface Register at a second relatively later gate pulse.

Abstract: An apparatus for and a method of providing error correction of the address word of a cache memory system (CMS) utilizing post-write storage of the least recently used (LRU) block of data words. Error correction circuitry (ECC) is provided at the output of the address buffer (CAB) portion of the cache memory system so that the address word that specifies the addressable location in the main storage unit (MSU) into which the block of data words, which block of data words is stored in the data buffer (CDB) portion of the cache memory, is to be stored or written-back is error corrected upon readout. This error correction of the address word ensures that correctable errors in the address buffer provided address words do not generate a Miss signal by the storage interface unit (SIU) which, in turn, requires a MSU reference even though the desired address word and the associated data word are available in the cache memory system.

Abstract: A method of and an apparatus for performing, in a Cache memory system, the Priority determination of what Requestor, of R Requestors, is to be granted priority by the Priority Network while simultaneously comparing, in parallel, all of the R Requestors' addresses for a Match condition in R Cache memories. The Cache memory system incorporates a separate Cache memory or associative memory for each Requestor, each of which Cache memories is comprised of an Address Buffer or Search memory, in which the associated Requestors' addresses are stored, and a Data Buffer or Associated memory, in which the data that are associated with each of the Requestors' addresses are stored. Thus, while the Priority Request signals from all of the requesting Requestors are being coupled to the single Priority Network, each of the requesting Requestors' addresses is coupled to each of the requesting Requestor separately associated Cache memory.