Patents by Inventor James A. Lavigne
James A. Lavigne has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20170327855Abstract: The present invention provides process for treating crop kernels, comprising the steps of a) soaking kernels in water to produce soaked kernels; b) grinding the soaked kernels; c) treating the soaked kernels in the presence of an effective amount of an enzyme composition comprising: i) a protease, and ii) a cellulolytic composition, wherein step c) is performed before, during or after step b).Type: ApplicationFiled: November 26, 2015Publication date: November 16, 2017Applicant: Novozymes A/SInventors: Zhen Long, James Lavigne, JR., Bernardo Vidal, JR., Brian R. Scott, Randall Scott Deinhammer, Thomas Patrick Gibbons, Chee-Leong Soong, Yi Cao, Yu Zhang
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Publication number: 20170101635Abstract: The present invention relates to GH Family 11 xylanase variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.Type: ApplicationFiled: May 22, 2015Publication date: April 13, 2017Applicant: NOVOZYMES A/SInventors: James Lavigne, Brian R. Scott, Daniel Sebastian Kolczynski
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Publication number: 20170096687Abstract: The present invention relates to xylanase variants, comprising an alteration at least at one position corresponding to position 87 of the polypeptide of SEQ ID NO: 3, wherein the variant has xylanase activity and has increased xylanase inhibitor tolerance compared to the xylanase of SEQ ID NO: 3; and i) wherein the variant has at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the polypeptide of SEQ ID NO: 3; or ii) wherein the number of alterations is 1-20, e.g., 1-10 such as 1, 2, 3, 4, 5, 6, 7, 8, 9 or 10 alterations. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.Type: ApplicationFiled: June 25, 2015Publication date: April 6, 2017Applicant: NOVOZYMES A/SInventors: Brian R. Scott, Mark David Wogulis, Sven Pedersen, James Lavigne
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Publication number: 20160326507Abstract: The present invention relates to GH Family 5 endoglucanase variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.Type: ApplicationFiled: January 22, 2015Publication date: November 10, 2016Applicant: NOVOZYMES A/SInventors: James Lavigne, Daniel Sebastian Kolczynski, Brian R. Scott
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Publication number: 20160201043Abstract: Provided are modified cellulase enzymes exhibiting increase cellulose-hydrolyzing activity in the presence of lignin and/or reduced binding to lignin comprising modified linker peptides comprising one or more amino acid substitutions, insertions, or deletions that result in (a) a decrease in the calculated isoelectric point of the linker peptide and/or (b) an increase in the ratio of threonine:serine in the linker peptide relative to a parental linker peptide from which said modified linker peptide is derived. Also provided are genetic constructs comprising nucleic acid sequences encoding for modified cellulase enzymes, methods for the production of the modified cellulase enzymes from host strains and a process for hydrolysing cellulose with the modified cellulases in the presence of lignin.Type: ApplicationFiled: January 25, 2016Publication date: July 14, 2016Inventors: Brian R. Scott, Patrick St-Pierre, James Lavigne, Nabil Masri, Theresa C. White, John J. Tomashek
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Patent number: 9279112Abstract: Provided are modified cellulase enzymes exhibiting increase cellulose-hydrolyzing activity in the presence of lignin and/or reduced binding to lignin comprising modified linker peptides comprising one or more amino acid substitutions, insertions, or deletions that result in (a) a decrease in the calculated isoelectric point of the linker peptide and/or (b) an increase in the ratio of threonine:serine in the linker peptide relative to a parental linker peptide from which said modified linker peptide is derived. Also provided are genetic constructs comprising nucleic acid sequences encoding for modified cellulase enzymes, methods for the production of the modified cellulase enzymes from host strains and a process for hydrolyzing cellulose with the modified cellulases in the presence of lignin.Type: GrantFiled: February 26, 2010Date of Patent: March 8, 2016Assignee: IOGEN ENERGY CORPORATIONInventors: Brian R. Scott, Patrick St-Pierre, James Lavigne, Nabil Masri, Theresa C. White, John J. Tomashek
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Patent number: 9279163Abstract: Provided are isolated cellobiohydrolases comprising a modified Glycoside Hydrolase (GH) Family 7 catalytic domain, a GH Family 7 catalytic domain and a modified Family 1 carbohydrate binding module (CBM), or both a modified Family 7 catalytic domain and a modified Family 1 CBM. Such isolated cellobiohydrolases exhibit from 45% to about 99.9% amino acid sequence identity to amino acids 1-436 of SEQ ID NO: 1 or to amino acids 1-438 of SEQ ID NO: 2 and improved activity on process substrates. Also provided are genetic constructs and genetically modified microbes for expressing the isolated cellobiohydrolases, a process for producing the isolated cellobiohydrolases, cellulase enzyme mixtures comprising the isolated cellobiohydrolase and a process for hydrolyzing a cellulosic substrate with such cellulase enzyme mixtures.Type: GrantFiled: August 24, 2012Date of Patent: March 8, 2016Assignee: IOGEN ENERGY CORPORATIONInventors: Jacqueline Montalibet, Loreta Gudynaite-Savitch, Christopher Hill, Christopher D. Hindle, James A. Lavigne, Nabil Masri, Fuad Tahna, John J. Tomashek
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Publication number: 20150329841Abstract: The present invention relates to a cellulose-degrading enzyme composition comprising one or more cellobiohydrolase and/or endoglucanase enzyme, and an effective amount of an isolated GH16 polypeptide, where the presence of the isolated GH16 polypeptide in the enzyme composition increases the rate or extent of degradation of a cellulosic substrate compared to an equivalent dosage of a corresponding cellulose-degrading enzyme composition lacking the at least one isolated GH16 polypeptide. The present invention also relates to a method for producing fermentable sugars from a cellulosic substrate using the above cellulose-degrading enzyme composition and to genetically modified microbes for produced the above cellulose-degrading enzyme composition.Type: ApplicationFiled: May 31, 2013Publication date: November 19, 2015Inventors: Christopher M. D. Hill, John J. Tomashek, James A. Lavigne, Sandra Mortimer, Reginald Storms, Adrian Tsang, Greg Butler, Justin Powlowski
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Patent number: 8486683Abstract: Provided are modified beta-glucosidase enzymes, derived from the Trichoderma reesei Cel3A beta-glucosidase, that exhibit improvements in one or more kinetic parameters (KG, KG2, kcat) comprising amino acid substitutions at one or more of positions 43, 101, 260 and 543. Also provided are genetic constructs comprising nucleotide sequences encoding for modified beta-glucosidase enzymes, methods for the production of modified beta-glucosidase enzymes from host strains and the use of the modified beta-glucosidase enzymes in the hydrolysis of cellulose.Type: GrantFiled: May 28, 2010Date of Patent: July 16, 2013Assignee: Iogen Energy CorporationInventors: Brian R. Scott, Chengsong Liu, James Lavigne, John J. Tomashek
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Publication number: 20130052694Abstract: Provided are isolated cellobiohydrolases comprising a modified Glycoside Hydrolase (GH) Family 7 catalytic domain, a GH Family 7 catalytic domain and a modified Family 1 carbohydrate binding module (CBM), or both a modified Family 7 catalytic domain and a modified Family 1 CBM. Such isolated cellobiohydrolases exhibit from 45% to about 99.9% amino acid sequence identity to amino acids 1-436 of SEQ ID NO: 1 or to amino acids 1-438 of SEQ ID NO: 2 and improved activity on process substrates. Also provided are genetic constructs and genetically modified microbes for expressing the isolated cellobiohydrolases, a process for producing the isolated cellobiohydrolases, cellulase enzyme mixtures comprising the isolated cellobiohydrolase and a process for hydrolysing a cellulosic substrate with such cellulase enzyme mixtures.Type: ApplicationFiled: August 24, 2012Publication date: February 28, 2013Applicant: Iogen Energy CorporationInventors: Jacqueline Montalibet, Loreta Gudynaite-Savitch, Christopher Hill, Christopher D. Hindle, James A. Lavigne, Nabil Masri, Fuad Tahna, John J. Tomashek
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Patent number: 8263379Abstract: A modified Family 6 glycosidase enzyme comprising amino acid substitutions at one or more positions selected from the group 182, 367, 399, 400 and 427 is provided (the position determined form alignment of a parental Family 6 glycosidase with SEQ ID NO: 1). Genetic constructs and genetically modified microbes comprising nucleic sequences encoding the modified Family 6 glycosidase are also provided. Family 6 glycosidase of the invention display decreased hydrolysis activity of beta 1-4 linked polysaccharides and increased hydrolysis activity of beta 1-3, 1-4 linked polysaccharides compared with a parental Family 6 glycosidase. Such glycosidases find use in a variety of applications in industry, e.g., in hydrolysis of beta 1-3, 1-4 linked polysaccharides during the processing of cereal grains or the production of alcohol, animal feed or food products.Type: GrantFiled: July 16, 2009Date of Patent: September 11, 2012Assignee: Iogen Energy CorporationInventors: John Tomashek, James Lavigne, Annie Tremblay, Patrick St-Pierre
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Patent number: 8143049Abstract: Provided are modified beta-glucosidase enzymes, derived from the Trichoderma reesei Cel3A beta-glucosidase, that exhibit improved stability at low pH, low pH and high aeration, low pH and high agitation, or low pH and elevated temperature. Also provided are genetic constructs comprising nucleotide sequences encoding for modified beta-glucosidase enzymes, methods for the production of modified beta-glucosidase enzymes from host strains and the use of the modified beta-glucosidase enzymes in the hydrolysis of cellulose.Type: GrantFiled: August 31, 2009Date of Patent: March 27, 2012Assignee: Iogen Energy CorporationInventors: Christopher Hill, James Lavigne, Martine Whissel, John J. Tomashek
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Patent number: 8110389Abstract: A modified Trichoderma reesei Family 6 (TrCel6A) cellulase enzyme comprising amino acid substitutions at one or more positions selected from the group consisting of 129, 322, 363 and 410 of SEQ ID NO: 1 is provided. Genetic constructs and genetically modified microbes comprising nucleic sequences encoding the modified TrCel6a cellulase are also provided. The modified TrCel6A cellulase of the invention display at least a 15% decrease in inactivation by lignin relative to a parental TrCel6A cellulase from which the modified TrCel6A is derived. Such cellulases find use in a variety of applications in industry requiring enzymatic hydrolysis of cellulose in the presence of lignin, e.g., the hydrolysis of pretreated lignocellulosic feedstocks for the production of fermentable sugars, sugar alcohols and fuel alcohols.Type: GrantFiled: July 30, 2009Date of Patent: February 7, 2012Assignee: Iogen Energy CorporationInventors: James A. Lavigne, Brian R. Scott, Martine Whissel, John J. Tomashek
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Patent number: 8012734Abstract: A Family 6 cellulase variant enzyme comprising one or more than one amino acid substitution selected from a basic, polar or non-polar amino acid at position 103, a valine or isoleucine at position 136, a tyrosine at position 186, a glutamic acid or glutamine at position 365 and a glutamine at position 410 is provided (said position determined form alignment of the parental Family 6 with SEQ ID NO: 1). Genetic constructs and genetically modified microbes comprising DNA sequences encoding the Family 6 cellulase variant are also provided. Family 6 cellulases of the invention display reduced inhibition by glucose relative to the parent Family 6 cellulases. Such cellulases find use in a variety of applications in industry, e.g., in the hydrolysis of pretreated lignocellulosic feedstock, that require cellulose activity in the presence glucose concentrations that would otherwise inhibit the activity of the parental enzyme.Type: GrantFiled: January 16, 2009Date of Patent: September 6, 2011Assignee: Iogen Energy CorporationInventors: James A. Lavigne, Christopher M. D. Hill, Annie Tremblay, Patrick St-Pierre, John J. Tomashek
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Publication number: 20100304438Abstract: Provided are modified beta-glucosidase enzymes, derived from the Trichoderma reesei Cel3A beta-glucosidase, that exhibit improvements in one or more kinetic parameters (KG, KG2, kcat) comprising amino acid substitutions at one or more of positions 43, 101, 260 and 543. Also provided are genetic constructs comprising nucleotide sequences encoding for modified beta-glucosidase enzymes, methods for the production of modified beta-glucosidase enzymes from host strains and the use of the modified beta-glucosidase enzymes in the hydrolysis of cellulose.Type: ApplicationFiled: May 28, 2010Publication date: December 2, 2010Applicant: Iogen Energy CorporationInventors: Brian R. Scott, Chengsong Liu, James Lavigne, John J. Tomashek
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Publication number: 20100221778Abstract: Provided are modified cellulase enzymes exhibiting increase cellulose-hydrolyzing activity in the presence of lignin and/or reduced binding to lignin comprising modified linker peptides comprising one or more amino acid substitutions, insertions, or deletions that result in (a) a decrease in the calculated isoelectric point of the linker peptide and/or (b) an increase in the ratio of threonine:serine in the linker peptide relative to a parental linker peptide from which said modified linker peptide is derived. Also provided are genetic constructs comprising nucleic acidsequences encoding for modified cellulase enzymes, methods for the production of the modified cellulase enzymes from host strains and a process for hydrolysing cellulose with the modified cellulases in the presence of lignin.Type: ApplicationFiled: February 26, 2010Publication date: September 2, 2010Applicant: IOGEN ENERGY CORPORATIONInventors: Brian R. Scott, Patrick St-Pierre, James Lavigne, Nabil Masri, Theresa C. White, John J. Tomashek
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Publication number: 20100093040Abstract: Provided are modified beta-glucosidase enzymes, derived from the Trichoderma reesei Cel3A beta-glucosidase, that exhibit improved stability at low pH, low pH and high aeration, low pH and high agitation, or low pH and elevated temperature. Also provided are genetic constructs comprising nucleotide sequences encoding for modified beta-glucosidase enzymes, methods for the production of modified beta-glucosidase enzymes from host strains and the use of the modified beta-glucosidase enzymes in the hydrolysis of cellulose.Type: ApplicationFiled: August 31, 2009Publication date: April 15, 2010Applicant: IOGEN ENERGY CORPORATIONInventors: Christopher Hill, James Lavigne, Martine Whissel, John J. Tomashek
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Publication number: 20100041100Abstract: A modified Trichoderma reesei Family 6 (TrCel6A) cellulase enzyme comprising amino acid substitutions at one or more positions selected from the group consisting of 129, 322, 363 and 410 of SEQ ID NO: 1 is provided. Genetic constructs and genetically modified microbes comprising nucleic sequences encoding the modified TrCel6a cellulase are also provided. The modified TrCel6A cellulase of the invention display at least a 15% decrease in inactivation by lignin relative to a parental TrCel6A cellulase from which the modified TrCel6A is derived. Such cellulases find use in a variety of applications in industry requiring enzymatic hydrolysis of cellulose in the presence of lignin, e.g., the hydrolysis of pretreated lignocellulosic feedstocks for the production of fermentable sugars, sugar alcohols and fuel alcohols.Type: ApplicationFiled: July 30, 2009Publication date: February 18, 2010Applicant: IOGEN ENERGY CORPORATIONInventors: James A. Lavigne, Brian R. Scott, Martine Whissel, John J. Tomashek
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Publication number: 20100016570Abstract: A modified Family 6 glycosidase enzyme comprising amino acid substitutions at one or more positions selected from the group 182, 367, 399, 400 and 427 is provided (the position determined form alignment of a parental Family 6 glycosidase with SEQ ID NO: 1). Genetic constructs and genetically modified microbes comprising nucleic sequences encoding the modified Family 6 glycosidase are also provided. Family 6 glycosidase of the invention display decreased hydrolysis activity of beta 1-4 linked polysaccharides and increased hydrolysis activity of beta 1-3, 1-4 linked polysaccharides compared with a parental Family 6 glycosidase. Such glycosidases find use in a variety of applications in industry, e.g., in hydrolysis of beta 1-3, 1-4 linked polysaccharides during the processing of cereal grains or the production of alcohol, animal feed or food products.Type: ApplicationFiled: July 16, 2009Publication date: January 21, 2010Applicant: IOGEN ENERGY CORPORATIONInventors: John Tomashek, James Lavigne, Annie Tremblay, Patrick St-Pierre
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Publication number: 20090186381Abstract: A Family 6 cellulase variant enzyme comprising one or more than one amino acid substitution selected from a basic, polar or non-polar amino acid at position 103, a valine or isoleucine at position 136, a tyrosine at position 186, a glutamic acid or glutamine at position 365 and a glutamine at position 410 is provided (said position determined form alignment of the parental Family 6 with SEQ ID NO: 1). Genetic constructs and genetically modified microbes comprising DNA sequences encoding the Family 6 cellulase variant are also provided. Family 6 cellulases of the invention display reduced inhibition by glucose relative to the parent Family 6 cellulases. Such cellulases find use in a variety of applications in industry, e.g., in the hydrolysis of pretreated lignocellulosic feedstock, that require cellulose activity in the presence glucose concentrations that would otherwise inhibit the activity of the parental enzyme.Type: ApplicationFiled: January 16, 2009Publication date: July 23, 2009Applicant: IOGEN ENERGY CORPORATIONInventors: James A. Lavigne, Christopher M.D. Hill, Annie Tremblay, Patrick St-Pierre, John J. Tomashek