Abstract: The present application provides engineered polypeptides having imine reductase activity, polynucleotides encoding the engineered polypeptides, host cells capable of expressing the engineered polypeptides, and methods of using these engineered polypeptides with a range of ketone and amine substrate compounds to prepare secondary and tertiary amine product compounds.

Abstract: The present application provides engineered glucose dehydrogenase polypeptides having imine reductase activity, polynucleotides encoding the engineered polypeptides, host cells capable of expressing the engineered polypeptides, and methods of using these engineered polypeptides with a range of ketone and amine substrate compounds to prepare secondary and tertiary amine product compounds.

Abstract: Systems and methods are provided to generate an access code specific to a user of an online marketplace to use to enter a first accommodation during a first reservation time frame and a second accommodation during a second reservation time frame and to send the access code specific to the user to a computing device at the first accommodation and a computing device at the second accommodation to use to enter the first accommodation during the first reservation time period and the second accommodation during the second reservation time period. The access code is automatically removed from the computing device at the first accommodation and the computing device at the second accommodation at the end of the first reservation time period and at the end of the second reservation time period, respectively.

Abstract: A vehicle determines that a vehicle alarm system has been engaged and determines a local context applicable to the vehicle alarm system. The vehicle obtains an alarm system configuration mapping applicable in light of the local context. The vehicle changes sensitivity of one or more sensors associated with the alarm system based on the configuration mapping.

Abstract: According to one aspect, an apparatus includes a first set of sensors, a second set of sensors, a sensor data aggregator that includes a first assembly and a second assembly, and a computing system. The first assembly is configured to obtain and to process a first set of sensor data from the first set of sensors, and the second assembly is configured to obtain and to process a second set of sensor data from the second set of sensors. The computing system is configured to obtain the first and second sets of sensor data processed by the sensor data aggregator, and to implement primary autonomy functionalities based on the first set of sensor data and the second set of sensor data, wherein the sensor data aggregator is configured to implement backup autonomy functionalities concurrently with the primary autonomy functionalities being implemented.

Abstract: The techniques and systems herein enable robot management in populated areas. Specifically, a robot management system receives, from a robot system, a requested route and time for the robot system to travel. A dynamic map that includes dynamic and temporal information about an area of the requested route is then used to determine whether the requested route and time are approved, approved with a modified route or time, or disapproved. An approval message, a modified approval message, or a disapproval message is then generated and sent to the robot system. By using the described techniques, municipalities can monitor, manage, and regulate robots across various operators and locations. Doing so may prevent a high density of robots along various routes, disruptions to the services of such robots, and/or disruptions to surrounding pedestrians and vehicles.

Abstract: The present application provides engineered polypeptides having imine reductase activity, polynucleotides encoding the engineered polypeptides, host cells capable of expressing the engineered polypeptides, and methods of using these engineered polypeptides with a range of ketone and amine substrate compounds to prepare secondary and tertiary amine product compounds.

Abstract: The present application provides engineered glucose dehydrogenase polypeptides having imine reductase activity, polynucleotides encoding the engineered polypeptides, host cells capable of expressing the engineered polypeptides, and methods of using these engineered polypeptides with a range of ketone and amine substrate compounds to prepare secondary and tertiary amine product compounds.

Abstract: This document describes techniques for localizing a vehicle to map data, for example, to enable assisted driving and automated driving within a road. A road model builder is described that uses vehicle location data to determine its position relative to map data obtained from a provider, which can be different than the location data's source. Using the vehicle position, the road model builder maintains a current lane segment for the vehicle and its relative position within the current lane segment. Based on its relative position, the road model builder determines whether a transition to another lane segment is appropriate before setting the current lane segment to be the other lane segment. The current lane segment, when provided as an input to an assisted-driving or automated-driving system, can enable safer operation of the vehicle and use fewer computing resources than other systems.

Abstract: A method of processing a transaction between a first user associated with a first user device and a second user, associated with a second user device within a transaction system. The method may include establishing a communication channel between the first and second user devices; sending, from the first user device to the second user device over the communication channel, a transaction message to initiate the transaction between the first and second users, the transaction message including transaction data; receiving the transaction message at the second user device; validating the transaction data within the received transaction message; sending, from the second user device to the first user device over the communication channel, a confirmation transaction message, the confirmation transaction message confirming that the transaction has been approved by the first user and second user; sending the confirmation transaction message to the transaction system.

Abstract: The present invention provides engineered RNA polymerase variants and compositions comprising these variants. The present invention further provides engineered T7 RNA polymerase variants and compositions comprising these variants. These variants have been evolved for selective incorporation of the m7G(5?)ppp(5?)m7G cap analog over GTP at the initiation of in vitro transcription. The present invention also provides methods for selective capping of RNA transcripts.

Abstract: Aspects described herein relate to a method comprising: receiving a request to write data to a persistent storage device, the request comprising data; determining an affinity of the data; writing the request to a cache line of a cache; associating the cache line with the affinity of the data; and reporting the data as having been written to the persistent storage device.

Abstract: Aspects of the disclosure provide for pre-deal and in-deal or live demand and allocation forecasting. For instance, historical data including information about prior completed offers may be accessed and used to generate a plurality of training input features and a plurality of training output features. These may be used to train a pre-deal demand model configured to generate a demand prediction for a new offer based on a plurality of input features for the new offer. In addition, these may be used to train a live demand model configured to generate a demand prediction for an offer while the offer is open based on a plurality of input features for the offer. Still further, a plurality of potential allocation policies to the demand prediction to generate an allocation in real time while the offer is open.

Abstract: An aftertreatment system for treating exhaust gas includes: an exhaust conduit configured to receive the exhaust gas; a preheating oxidation catalyst disposed in the exhaust conduit; a primary oxidation catalyst disposed in the exhaust conduit downstream of the preheating oxidation catalyst; a selective catalytic reduction system disposed in the exhaust conduit downstream of the primary oxidation catalyst; and a controller configured to: determine a temperature of the exhaust gas at an inlet of the selective catalytic reduction system, and in response to the temperature of the exhaust gas at the inlet of the selective catalytic reduction system being below a threshold temperature, cause hydrocarbons to be provided to the preheating oxidation catalyst or to the exhaust conduit at a location upstream of the preheating oxidation catalyst. The preheating oxidation catalyst catalyzes combustion of the hydrocarbons so as to increase the temperature of the exhaust gas to above the threshold temperature.

Abstract: The present invention provides engineered penicillin G acylase (PGA) enzymes, polynucleotides encoding the enzymes, compositions comprising the enzymes, and methods of using the engineered PGA enzymes.

Abstract: Techniques are described for learning an unknown virtual network information, such as an virtual Internet Protocol (IP) address, of a pod in a virtual network. In some examples, a virtual router executing at a computing device may receive an Address Resolution Protocol (ARP) packet from a virtual execution element in the virtual network, the virtual execution element executing at the computing device. The virtual router may determine, based at least in part on the ARP packet, whether virtual network information for the virtual execution element in a virtual network is known to the virtual router. The virtual router may, in response to determining that the virtual network information of the virtual execution element in the virtual network is not known to the virtual router, perform learning of the virtual network information for the virtual execution element.

Abstract: The present invention provides engineered RNA polymerase variants and compositions comprising these variants. The present invention further provides engineered T7 RNA polymerase variants and compositions comprising these variants. These variants have been evolved for selective incorporation of the m7G(5?)ppp(5?)m7G cap analog over GTP at the initiation of in vitro transcription. The present invention also provides methods for selective capping of RNA transcripts.

Abstract: A method for real-time invoice updating and account-to-account payment includes: receiving invoice data based on presentation of an invoice by a distributor to a recipient at a delivery location, modification of the invoice by the recipient, and acceptance of the invoice by the recipient; generating a request for payment (RFP) message based on the invoice data; transmitting the RFP message to a financial institution associated with the recipient via a financial institution of the distributor; receiving, from the financial institution associated with the distributor, a payment confirmation for the invoice based on a real-time payment from the financial institution associated with the recipient; generating reconciliation data based on the payment confirmation and the invoice data; and transmitting the reconciliation data to a computing device of the distributor and a computing device of the recipient.

Abstract: This disclosure describes one or more embodiments of methods, non-transitory computer-readable media, and systems that utilize computing models to intelligently match provider devices and requester devices in response to event-trigger transportation requests. In particular, in one or more embodiments, the disclosed systems utilize global positioning information to monitor requester device proximity to pickup locations and generate timely transportation matches for event-trigger transportation requests. For example, the event-trigger transportation matching system matches requester devices and provider devices based on the satisfaction of a location trigger; or, conversely, the event-trigger transportation matching system delays matching processes for provider devices based on a location trigger remaining unsatisfied.