Abstract: A method of assessing the risk in female patients of having neurocognitive developmentally disabled children. The method including measuring the toxin concentrations in the urine of a female subject wishing to become pregnant, or of a subject that is pregnant. Quantify the risk of neurocognitive developmentally disabled children born to the subject by comparing the toxin concentrations in the subject to reference concentrations utilizing a weighting algorithm. Advise the patient of the quantified risk of having neurocognitive developmentally disabled children. The concentrations of dialkyl phosphates, endocrine disruptors, and xenoestrogens compounds are evaluated. Additionally, the level of PON1 activity may be included in the risk assessment weighting algorithm.

Abstract: Disclosed are methods for monitoring the gastrointestinal tract of the human gastrointestinal system. The method includes: 1) grouping microbes into specific operational taxonomic units (OTU); 2) using oligonucleotide probes and PCR primers to detect and quantify specific microbes (bacteria, fungi/yeast, protozoans and parasitic worms) in human fecal material. The inventions also discloses a kit that includes: a DNA isolation step; 2) accumulation of specific operational taxonomic units (OTU); 3) identification and quantifying of sequences internal to the OTU; 4) reporting changes the indigenous population of the human gastrointestinal system.

Abstract: Nutritional supplements and methods for formulating and customizing such nutritional supplements are included. Also included are methods for formulating and customizing nutritional supplements to subject deficiencies for preventing disease and protecting against nutritional losses and deficiencies.

Abstract: This invention particularly discloses an improved immunoassay method for the sensitive and specific detection of food specific human IgG4 antibodies. A sample diluent comprising a chaotrophic agent is used to reduce the occurrence of nonspecific antibody-dietary antigen interactions. To reduce competition between IgE and IgG4 antibodies for specific epitopes on dietary antigens a heat denaturing step is included to inactivate IgE antibodies. Finally, a signal amplification step is included in the assay to reduce the amount of sample required to perform the assay.

Abstract: A method for the separation and simultaneous determination of urinary D- and L-lactic acid enantiomers by high performance liquid chromatography tandem mass spectrometry (HPLC/MS/MS). The chiral separation was done using a chirobiotic teicoplanin aglycone column varying mobile phase parameters such as the acetic acid/triethylamine content and the aqueous/organic ratio.