Patents by Inventor James Anthony Clarke

James Anthony Clarke has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20220208427
    Abstract: There is provided a multipole magnet for deflecting a beam of charged particles. The multipole magnet comprises a plurality of ferromagnetic poles and a plurality of permanent magnet assemblies to supply a magnetomotive force to the ferromagnetic poles. At least one of the permanent magnet assemblies has a plurality of discrete permanent magnet positions and a plurality of permanent magnets each fixed in one of the permanent magnet positions.
    Type: Application
    Filed: March 18, 2020
    Publication date: June 30, 2022
    Inventors: James Anthony CLARKE, Benjamin John Arthur SHEPHERD, Norbert COLLOMB, Alexander Robert BAINBRIDGE
  • Patent number: 11261487
    Abstract: The claimed invention relates to a method of processing a polynucleotide, by obtaining a sense polynucleotide strand comprising a homopolymeric region that is longer that the reading section of a nanopore; synthesizing an antisense polynucleotide strand under conditions in which a nucleotide analog is incorporated at random in a reverse complement of the homopolymer region, such that the length of the homopolymer region in the antisense polynucleotide strand is shorter than the reading section of the nanopore; and moving the antisense polynucleotide strand through the nanopore such that a proportion of the antisense polynucleotide strand interacts with the nanopore.
    Type: Grant
    Filed: February 5, 2020
    Date of Patent: March 1, 2022
    Assignee: Oxford Nanopore Technologies PLC
    Inventors: Clive Gavin Brown, James Anthony Clarke, Graham Hall, Gavin Harper, Andrew John Heron, James White
  • Publication number: 20210363577
    Abstract: Methods of characterizing an analyte using a detector such as a nanopore and an enzyme are provided. One aspect features methods for characterizing a double-stranded polynucleotide using a detector, e.g., without using a hairpin connecting a template and a complement of the double-stranded polynucleotide. Another aspect features methods for characterizing an analyte using a tag-modified nanopore with increased sensitivity and/or higher throughput. Compositions and systems including, e.g., adaptors for attachment to double-stranded polynucleotides and tag-modified nanopores, which can be used in the methods are also provided.
    Type: Application
    Filed: June 6, 2019
    Publication date: November 25, 2021
    Applicant: Oxford Nanopore Technologies Limited
    Inventors: James Anthony Clarke, James White, Richard Muscat, Jessica Mary May Johnson, Ramiz Iqbal Nathani, Andrew John Heron, Mark John Bruce, Lakmal Nishantha Jayasinghe, Domenico Caprotti, David Jackson Stoddart, Rebecca Victoria Bowen, Christopher James Wright, Paul Richard Moody
  • Patent number: 11168363
    Abstract: The invention relates to a new method of sequencing a double stranded target polynucleotide. The two strands of the double stranded target polynucleotide are linked by a bridging moiety. The two strands of the target polynucleotide are separated using a polynucleotide binding protein and the target polynucleotide is sequenced using a transmembrane pore.
    Type: Grant
    Filed: May 31, 2019
    Date of Patent: November 9, 2021
    Assignee: Oxford Nanopore Technologies Ltd.
    Inventors: Clive Gavin Brown, James Anthony Clarke, Graham Hall, Gavin Harper, Andrew John Heron, James White
  • Publication number: 20210324020
    Abstract: The invention relates to mutant forms of lysenin. The invention also relates to analyte characterisation using lysenin.
    Type: Application
    Filed: December 1, 2020
    Publication date: October 21, 2021
    Applicant: Oxford Nanopore Technologies Limited
    Inventors: Mark Bruce, James Anthony Clarke, Andrew John Heron, Lakmal Jayasinghe, Elizabeth Jayne Wallace
  • Patent number: 11136623
    Abstract: The invention relates to a new method of determining the presence, absence or characteristics of an analyte. The analyte is coupled to a membrane. The invention also relates to nucleic acid sequencing.
    Type: Grant
    Filed: May 31, 2019
    Date of Patent: October 5, 2021
    Assignee: Oxford Nanopore Technologies Limited
    Inventors: James Anthony Clarke, James White, John Milton, Clive Gavin Brown
  • Patent number: 11078530
    Abstract: The invention relates to constructs comprising a transmembrane protein pore subunit and a nucleic acid handling enzyme. The pore subunit is covalently attached to the enzyme such that both the subunit and enzyme retain their activity. The constructs can be used to generate transmembrane protein pores having a nucleic acid handling enzyme attached thereto. Such pores are particularly useful for sequencing nucleic acids. The enzyme handles the nucleic acid in such a way that the pore can detect its component nucleotides by stochastic sensing.
    Type: Grant
    Filed: September 12, 2018
    Date of Patent: August 3, 2021
    Assignee: Oxford Nanopore Technologies Ltd.
    Inventors: Lakmal Jayasinghe, John Hagan Pryce Bayley, Stephen Cheley, Brian McKeown, James White, James Anthony Clarke
  • Patent number: 11041194
    Abstract: The invention relates to a new method of determining the presence, absence or characteristics of an analyte. The analyte is coupled to a membrane. The invention also relates to nucleic acid sequencing.
    Type: Grant
    Filed: January 9, 2019
    Date of Patent: June 22, 2021
    Assignee: Oxford Nanopore Technologies Ltd.
    Inventors: James Anthony Clarke, James White, John Milton, Clive Gavin Brown
  • Publication number: 20210180124
    Abstract: The invention relates to a new method of determining the presence, absence or characteristics of an analyte. The analyte is coupled to a membrane. The invention also relates to nucleic acid sequencing.
    Type: Application
    Filed: November 10, 2020
    Publication date: June 17, 2021
    Applicant: Oxford Nanopore Technologies Limited
    Inventors: James Anthony Clarke, James White, John Milton, Clive Gavin Brown
  • Patent number: 11034734
    Abstract: The invention relates to mutant forms of CsgG. The invention also related to analyte detection and characterisation using CsgG.
    Type: Grant
    Filed: July 25, 2019
    Date of Patent: June 15, 2021
    Assignees: Oxford Nanopore Technologies Ltd., VIB VZW, Vrije Universiteit Brussel
    Inventors: Stefan Howorka, Han Remaut, Lakmal Jayasinghe, Elizabeth Jayne Wallace, James Anthony Clarke, Richard George Hambley, Jonathan Bankes Pugh
  • Publication number: 20210095337
    Abstract: The invention relates to a new method of determining the presence, absence or characteristics of an analyte. The analyte is coupled to a membrane. The invention also relates to nucleic acid sequencing.
    Type: Application
    Filed: October 7, 2020
    Publication date: April 1, 2021
    Applicant: Oxford Nanopore Technologies Limited
    Inventors: James Anthony Clarke, James White, John Milton, Clive Gavin Brown
  • Publication number: 20210087623
    Abstract: The invention relates to a new method of determining the presence, absence or characteristics of an analyte. The analyte is coupled to a membrane. The invention also relates to nucleic acid sequencing.
    Type: Application
    Filed: October 7, 2020
    Publication date: March 25, 2021
    Applicant: Oxford Nanopore Technologies Limited
    Inventors: James Anthony Clarke, James White, John Milton, Clive Gavin Brown
  • Publication number: 20210087621
    Abstract: The invention relates to a new method of determining the presence, absence or one or more characteristics of multiple analytes. The invention concerns coupling a first analyte to a membrane containing a detector and investigating the first analyte using the detector. The invention also concerns coupling a second analyte to the membrane and investigating the second analyte. The first analyte is uncoupled form the membrane prior to investigating the second analyte. The invention also relates to polynucleotide sequencing.
    Type: Application
    Filed: August 14, 2020
    Publication date: March 25, 2021
    Applicant: Oxford Nanopore Technologies Ltd.
    Inventors: James Anthony Clarke, Marion Louise Crawford, James White
  • Publication number: 20210018500
    Abstract: The invention relates to a new method of determining in a sample the presence or absence of one or more analyte members of a group of two or more analytes. The invention therefore relates to a multiplex assay for determining the presence or absence of each analyte in a group of multiple analytes. The assay uses aptamers and transmembrane pores.
    Type: Application
    Filed: June 30, 2020
    Publication date: January 21, 2021
    Applicant: Oxford Nanopore Technologies Limited
    Inventors: Daniel John Turner, Daniel George Fordham, Roger Charles Gill, Clive Gavin Brown, Stuart Reid, James Anthony Clarke, James White
  • Publication number: 20210008557
    Abstract: Droplet interfaces are formed between droplets in an electro-wetting device comprising an array of actuation electrodes. Actuation signals are applied to selected actuation electrodes to place the droplets into an energised state in which the shape of the droplets is modified compared to a shape of the droplets in a lower energy state and to bring the two droplets into proximity. The actuation signals are then changed to lower the energy of the droplets into the lower energy state so that the droplets relax into the gap and the two droplets contact each other thereby forming a droplet interface. The use of sensing electrodes in the device permit electrical current measurements across the droplet interface. The sensing electrodes can be used for either (i) applying a reference signal during droplet actuation or (ii) recording electrical current measurements.
    Type: Application
    Filed: December 21, 2018
    Publication date: January 14, 2021
    Applicants: Oxford Nanopore Technologies Ltd., Sharp Life Science (EU) Ltd
    Inventors: Matthew HOLDEN, James WHITE, Andrew John HERON, James Anthony CLARKE, Jason Robert HYDE, Benjamin James HADWEN, Sally ANDERSON
  • Patent number: 10882889
    Abstract: The invention relates to mutant forms of lysenin. The invention also relates to analyte characterisation using lysenin.
    Type: Grant
    Filed: August 31, 2017
    Date of Patent: January 5, 2021
    Assignee: Oxford Nanopore Technologies Ltd.
    Inventors: Mark Bruce, James Anthony Clarke, Andrew John Heron, Lakmal Jayasinghe, Elizabeth Jayne Wallace
  • Patent number: 10851409
    Abstract: The invention relates to a new method of sequencing a double stranded target polynucleotide. The two strands of the double stranded target polynucleotide are linked by a bridging moiety. The two strands of the target polynucleotide are separated using a polynucleotide binding protein and the target polynucleotide is sequenced using a transmembrane pore.
    Type: Grant
    Filed: June 10, 2016
    Date of Patent: December 1, 2020
    Assignee: Oxford Nanopore Technologies Ltd.
    Inventors: Clive Gavin Brown, James Anthony Clarke, Graham Hall, Gavin Harper, Andrew John Heron, James White
  • Publication number: 20200318179
    Abstract: The invention relates to an improved method for characterising a template polynucleotide. The method involves using a polymerase to prepare a modified polynucleotide which makes it easier to characterise than the template polynucleotide.
    Type: Application
    Filed: April 22, 2020
    Publication date: October 8, 2020
    Applicant: Oxford Nanopore Technologies Ltd.
    Inventors: James Anthony Clarke, Marion Louise Crawford, James White
  • Patent number: 10774378
    Abstract: The invention relates to a new method of determining the presence, absence or one or more characteristics of multiple analytes. The invention concerns coupling a first analyte to a membrane containing a detector and investigating the first analyte using the detector. The invention also concerns coupling a second analyte to the membrane and investigating the second analyte. The first analyte is uncoupled from the membrane prior to investigating the second analyte. The invention also relates to polynucleotide sequencing.
    Type: Grant
    Filed: March 31, 2015
    Date of Patent: September 15, 2020
    Assignee: Oxford Nanopore Technologies Ltd.
    Inventors: James Anthony Clarke, Marion Louise Crawford, James White
  • Patent number: 10739341
    Abstract: The invention relates to a new method of determining in a sample the presence or absence of one or more analyte members of a group of two or more analytes. The invention therefore relates to a multiplex assay for determining the presence or absence of each analyte in a group of multiple analytes. The assay uses aptamers and transmembrane pores.
    Type: Grant
    Filed: February 14, 2013
    Date of Patent: August 11, 2020
    Assignee: Oxford Nanopore Technologies Limited
    Inventors: Daniel John Turner, Daniel George Fordham, Roger Charles Gill, Clive Gavin Brown, Stuart Reid, James Anthony Clarke, James White