Abstract: To form a layer separating two volumes of aqueous solution, there is used an apparatus comprising elements defining a chamber, the elements including a body of non-conductive material having formed therein at least one recess opening into the chamber, the recess containing an electrode. A pre-treatment coating of a hydrophobic fluid is applied to the body across the recess. Aqueous solution, having amphiphilic molecules added thereto, is flowed across the body to cover the recess so that aqueous solution is introduced into the recess from the chamber and a layer of the amphiphilic molecules forms across the recess separating a volume of aqueous solution introduced into the recess from the remaining volume of aqueous solution.

Abstract: A multipole magnet for deflecting a beam of charged particles, comprising: a plurality of ferromagnetic poles arranged in a pole plane; a plurality of permanent magnets each having a magnetisation direction, and each being arranged to supply magnetomotive force to the plurality of ferromagnetic poles to produce a magnetic field along the pole plane in a beamline space between the poles; and a plurality of ferromagnetic flux conducting members arranged to channel magnetic flux from at least one of the plurality of permanent magnets; wherein the multipole magnet comprises an even number of ferromagnetic poles, each pole being arranged to diametrically oppose another of the poles in the pole plane along a pole axis, wherein each of the plurality of permanent magnets is associated with at least one of the plurality of poles and the magnetisation direction of each permanent magnet isorientated in the pole plane at an angle of at least 45° relative to the pole axis of the associated pole.

Abstract: The invention relates to constructs comprising a transmembrane protein pore subunit and a nucleic acid handling enzyme. The pore subunit is covalently attached to the enzyme such that both the subunit and enzyme retain their activity. The constructs can be used to generate transmembrane protein pores having a nucleic acid handling enzyme attached thereto. Such pores are particularly useful for sequencing nucleic acids. The enzyme handles the nucleic acid in such a way that the pore can detect its component nucleotides by stochastic sensing.

Abstract: A multipole magnet for deflecting a beam of charged particles, comprising: a plurality of ferromagnetic poles arranged in a pole plane; a plurality of permanent magnets each having a magnetisation direction, and each being arranged to supply magnetomotive force to the plurality of ferromagnetic poles to produce a magnetic field along the pole plane in a beamline space between the poles; and a plurality of ferromagnetic flux conducting members arranged to channel magnetic flux from at least one of the plurality of permanent magnets; wherein the multipole magnet comprises an even number of ferromagnetic poles, each pole being arranged to diametrically oppose another of the poles in the pole plane along a pole axis, wherein each of the plurality of permanent magnets is associated with at least one of the plurality of poles and the magnetisation direction of each permanent magnet isorientated in the pole plane at an angle of at least 45° relative to the pole axis of the associated pole.

Abstract: To form a layer separating two volumes of aqueous solution, there is used an apparatus comprising elements defining a chamber, the elements including a body of non-conductive material having formed therein at least one recess opening into the chamber, the recess containing an electrode. A pre-treatment coating of a hydrophobic fluid is applied to the body across the recess. Aqueous solution, having amphiphilic molecules added thereto, is flowed across the body to cover the recess so that aqueous solution is introduced into the recess from the chamber and a layer of the amphiphilic molecules forms across the recess separating a volume of aqueous solution introduced into the recess from the remaining volume of aqueous solution.

Abstract: To form a layer separating two volumes of aqueous solution, there is used an apparatus comprising elements defining a chamber, the elements including a body of non-conductive material having formed therein at least one recess opening into the chamber, the recess containing an electrode. A pre-treatment coating of a hydrophobic fluid is applied to the body across the recess. Aqueous solution, having amphiphilic molecules added thereto, is flowed across the body to cover the recess so that aqueous solution is introduced into the recess from the chamber and a layer of the amphiphilic molecules forms across the recess separating a volume of aqueous solution introduced into the recess from the remaining volume of aqueous solution.