Patents by Inventor James Apgar
James Apgar has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20200392205Abstract: The present disclosure provides muteins of FVIII to which a biocompatible polymer may be attached to increase the circulatory half-life of the muteins, as well as conjugates of such muteins and biocompatible polymers.Type: ApplicationFiled: February 24, 2020Publication date: December 17, 2020Inventors: James Apgar, Debra Pittman, Mark Stahl, Laura Lin, Justin Cohen
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Patent number: 10570189Abstract: The present disclosure provides muteins of FVIII to which a biocompatible polymer may be attached to increase the circulatory half-life of the muteins, as well as conjugates of such muteins and biocompatible polymers.Type: GrantFiled: March 4, 2015Date of Patent: February 25, 2020Assignee: PFIZER INC.Inventors: James Apgar, Debra Pittman, Mark Stahl, Laura Lin, Justin Cohen
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Patent number: 10407742Abstract: Intein modified proteins, isolated nucleic acids encoding intein modified proteins, fragments of intein modified proteins, isolated nucleic acids encoding fragments of intein modified proteins, transgenic plants containing any of the foregoing, and antibodies recognizing epitopes on intein modified proteins are provided.Type: GrantFiled: November 5, 2010Date of Patent: September 10, 2019Assignee: Agrivida, Inc.Inventors: Binzhang Shen, Gabor Lazar, Humberto de la Vega, Philip Lessard, R. Michael Raab, James Apgar
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Patent number: 10196623Abstract: A method of predicting an intein insertion site in a protein that will lead to a switching phenotype is provided. The method includes identifying a plurality of C/T/S sites within the protein; selecting from the plurality of C/T/S/sites those that are ranked 0.75 or higher by a support vector machine, within ten angstroms of the active site of the protein, and at or near a loop-?-sheet junction or a loop-?-helix junction. A method of controlling protein activity and hosts including proteins with controlled activity are also provided. Also, intein modified proteins and plants containing intein modified proteins are provided.Type: GrantFiled: January 20, 2016Date of Patent: February 5, 2019Assignee: AGRIVIDA, INC.Inventors: Binzhang Shen, Gabor Lazar, Humberto de la Vega, James Apgar, Philip Lessard, R. Michael Raab
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Patent number: 9464333Abstract: A method of predicting an intein insertion site in a protein that will lead to a switching phenotype is provided. The method includes identifying a plurality of C/T/S sites within the protein; selecting from the plurality of C/T/S/ sites those that are ranked 0.75 or higher by a support vector machine, within ten angstroms of the active site of the protein, and at or near a loop-?-sheet junction or a loop-?-helix junction. A method of controlling protein activity and hosts including proteins with controlled activity are also provided. Also, intein modified proteins and plants containing intein modified proteins are provided.Type: GrantFiled: November 5, 2010Date of Patent: October 11, 2016Assignee: Agrivida, Inc.Inventors: James Apgar, Philip Lessard, R. Michael Raab, Binzhang Shen, Gabor Lazar, Humberto de la Vega
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Patent number: 9388422Abstract: In planta consolidated bioprocessing has the advantages of decreasing biomass pretreatment costs, utilizing excess plant protein production capacity for enzyme production, and decreasing mass transfer resistance of enzyme diffusion to its substrate. However, in planta expression of cell wall degrading (CWD) enzymes often leads to detrimental plant phenotypes that impact crop yield. To provide in planta CWD enzyme activity without any adverse phenotype, a thermostable xylanase, XynB (EC 3.2.1.8), was engineered with a thermoregulated intein, Tth-HB27 DnaE-1 (Tth intein), that controls its hydrolytic activity through conditional intein splicing. Maize plants expressing the heat inducible Tth intein-modified XynB developed normally, yet possessed enhanced post harvest glucose production from dried corn stover. Expression of CWD enzymes as dormant, intein-modified proteins that can be activated by heat treatment after harvest provides the basis for developing a novel cellulosic processing trait in plants.Type: GrantFiled: August 23, 2011Date of Patent: July 12, 2016Assignee: Agrivida, Inc.Inventors: Binzhang Shen, James Apgar, Oleg Bougri, R. Michael Raab
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Publication number: 20160186157Abstract: A method of predicting an intein insertion site in a protein that will lead to a switching phenotype is provided. The method includes identifying a plurality of C/T/S sites within the protein; selecting from the plurality of C/T/S/sites those that are ranked 0.75 or higher by a support vector machine, within ten angstroms of the active site of the protein, and at or near a loop-?-sheet junction or a loop-?-helix junction. A method of controlling protein activity and hosts including proteins with controlled activity are also provided. Also, intein modified proteins and plants containing intein modified proteins are provided.Type: ApplicationFiled: January 20, 2016Publication date: June 30, 2016Applicant: AGRIVIDA, INC.Inventors: BINZHANG SHEN, GABOR LAZAR, HUMBERTO DE LA VEGA, JAMES APGAR, PHILIP LESSARD, R. MICHAEL RAAB
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Patent number: 9303250Abstract: A method of predicting an intein insertion site in a protein that will lead to a switching phenotype is provided. The method includes identifying a plurality of C/T/S sites within the protein; selecting from the plurality of C/T/S/ sites those that are ranked 0.75 or higher by a support vector machine, within ten angstroms of the active site of the protein, and at or near a loop-?-sheet junction or a loop-?-helix junction. A method of controlling protein activity and hosts including proteins with controlled activity are also provided. Also, intein modified proteins and plants containing intein modified proteins are provided.Type: GrantFiled: February 26, 2013Date of Patent: April 5, 2016Assignee: Agrivida, Inc.Inventors: Binzhang Shen, Gabor Lazar, Humberto de la Vega, James Apgar, Philip Lessard, R. Michael Raab
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Publication number: 20150306234Abstract: The present disclosure provides muteins of FVIII to which a biocompatible polymer may be attached to increase the circulatory half-life of the muteins, as well as conjugates of such muteins and biocompatible polymers.Type: ApplicationFiled: March 4, 2015Publication date: October 29, 2015Inventors: James Apgar, Debra Pittman, Mark Stahl, Laura Lin, Justin Cohen
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Patent number: 8722727Abstract: Novel derivatives of enfumafungin are disclosed herein, along with' their pharmaceutically acceptable salts, hydrates and prodrugs. Also disclosed are compositions comprising such compounds, methods of preparing such compounds and method of using such compounds as antifungal agents and/or inhibitors of (1,3)-?-D-glucan synthase. The disclosed compounds, their pharmaceutically acceptable salts, hydrates and prodrugs, as well as compositions comprising such compounds, salts, hydrates and prodrugs, are useful for treating and/or preventing fungal infections and associated diseases and conditions.Type: GrantFiled: August 10, 2009Date of Patent: May 13, 2014Assignee: Seynix, Inc.Inventors: Mark L. Greenlee, Robert Wilkening, James Apgar, Donald Sperbeck, Kenneth J. Wildonger, Dongfang Meng, Dann L. Parker, Jr., Ahmed Mamai
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Publication number: 20130247251Abstract: A method of predicting an intein insertion site in a protein that will lead to a switching phenotype is provided. The method includes identifying a plurality of C/T/S sites within the protein; selecting from the plurality of C/T/S/ sites those that are ranked 0.75 or higher by a support vector machine, within ten angstroms of the active site of the protein, and at or near a loop-?-sheet junction or a loop-?-helix junction. A method of controlling protein activity and hosts including proteins with controlled activity are also provided. Also, intein modified proteins and plants containing intein modified proteins are provided.Type: ApplicationFiled: February 26, 2013Publication date: September 19, 2013Applicant: AGRIVIDA, INC.Inventors: Binzhang Shen, Gabor Lazar, Humberto de la Vega, James Apgar, Philip Lessard, R. Michael Raab
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Publication number: 20130203125Abstract: In planta consolidated bioprocessing has the advantages of decreasing biomass pretreatment costs, utilizing excess plant protein production capacity for enzyme production, and decreasing mass transfer resistance of enzyme diffusion to its substrate. However, in planta expression of cell wall degrading (CWD) enzymes often leads to detrimental plant phenotypes that impact crop yield. To provide in planta CWD enzyme activity without any adverse phenotype, a thermostable xylanase, XynB (EC 3.2.1.8), was engineered with a thermoregulated intein, Tth-HB27 DnaE-1 (Tth intein), that controls its hydrolytic activity through conditional intein splicing. Maize plants expressing the heat inducible Tth intein-modified XynB developed normally, yet possessed enhanced post harvest glucose production from dried corn stover. Expression of CWD enzymes as dormant, intein-modified proteins that can be activated by heat treatment after harvest provides the basis for developing a novel cellulosic processing trait in plants.Type: ApplicationFiled: August 23, 2011Publication date: August 8, 2013Applicant: AGRIVIDA, INC.Inventors: Binzhang Shen, James Apgar, Oleg Bougri, R. Michael Raab
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Patent number: 8420387Abstract: A method of predicting an intein insertion site in a protein that will lead to a switching phenotype is provided. The method includes identifying a plurality of C/T/S sites within the protein; selecting from the plurality of C/T/S/ sites those that are ranked 0.75 or higher by a support vector machine, within ten angstroms of the active site of the protein, and at or near a loop-?-sheet junction or a loop-?-helix junction. A method of controlling protein activity and hosts including proteins with controlled activity are also provided. Also, intein modified proteins and plants containing intein modified proteins are provided.Type: GrantFiled: November 6, 2009Date of Patent: April 16, 2013Assignee: Agrivida, Inc.Inventors: Binzhang Shen, Gabor Lazar, Humberto de la Vega, James Apgar, Philip Lessard, R. Michael Raab
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Publication number: 20130036517Abstract: A method of predicting an intein insertion site in a protein that will lead to a switching phenotype is provided. The method includes identifying a plurality of C/T/S sites within the protein; selecting from the plurality of C/T/S/ sites those that are ranked 0.75 or higher by a support vector machine, within ten angstroms of the active site of the protein, and at or near a loop-?-sheet junction or a loop-?-helix junction. A method of controlling protein activity and hosts including proteins with controlled activity are also provided. Also, intein modified proteins and plants containing intein modified proteins are provided.Type: ApplicationFiled: November 5, 2010Publication date: February 7, 2013Applicant: AGRIVIDA, INC.Inventors: James Apgar, Philip Lessard, R. Michael Raab, Binzhang Shen, Gabor Lazar, Humberto de la Vega
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Publication number: 20130007919Abstract: Intein modified proteins, isolated nucleic acids encoding intein modified proteins, fragments of intein modified proteins, isolated nucleic acids encoding fragments of intein modified proteins, transgenic plants containing any of the foregoing, and antibodies recognizing epitopes on intein modified proteins are providedType: ApplicationFiled: November 5, 2010Publication date: January 3, 2013Applicant: AGRIVIDA, INC.Inventors: Binzhang Shen, Gabor Lazar, Humberto de la Vega, Philip Lessard, R. Michael Raab, James Apgar
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Patent number: 8188085Abstract: Novel derivatives of enfumafungin are disclosed herein, along with their pharmaceutically acceptable salts, hydrates and prodrugs. Also disclosed are compositions comprising such compounds, methods of preparing such compounds and methods of using such compounds as antifungal agents and/or inhibitors of (1,3)-?-D-glucan synthase. The disclosed compounds, their pharmaceutically acceptable salts, hydrates and prodrugs, as well as compositions comprising such compounds, salts, hydrates and prodrugs, are useful for treating and/or preventing fungal infections and associated diseases and conditions.Type: GrantFiled: August 7, 2009Date of Patent: May 29, 2012Assignees: Merck Sharp & Dohme Corp., Seynexis, Inc.Inventors: Mark L. Greenlee, Robert Wilkening, James Apgar, Donald Sperbeck, Kenneth J. Wildonger, Dongfang Meng, Dann L. Parker, Jr., Gregory J. Pacofsky, Brian H. Heasley, Ahmed Mamai, Kingsley Nelson
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Publication number: 20110224228Abstract: Novel derivatives of enfumafungin are disclosed herein, along with' their pharmaceutically acceptable salts, hydrates and prodrugs. Also disclosed are compositions comprising such compounds, methods of preparing such compounds and method of using such compounds as antifungal agents and/or inhibitors of (1,3)-?-D-glucan synthase. The disclosed compounds, their pharmaceutically acceptable salts, hydrates and prodrugs, as well as compositions comprising such compounds, salts, hydrates and prodrugs, are useful for treating and/or preventing fungal infections and associated diseases and conditions.Type: ApplicationFiled: August 10, 2009Publication date: September 15, 2011Applicants: Merck Sharp & Dohme Corp., Scynexis, Inc.Inventors: Mark L Greenlee, Robert Wilkening, James Apgar, Donald Sperbeck, Kenneth J. Wildonger, Dongfang Meng, Dann L. Parker, JR., Ahmed Mamai
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Publication number: 20110111442Abstract: A method of predicting an intein insertion site in a protein that will lead to a switching phenotype is provided. The method includes identifying a plurality of C/T/S sites within the protein; selecting from the plurality of C/T/S/ sites those that are ranked 0.75 or higher by a support vector machine, within ten angstroms of the active site of the protein, and at or near a loop-?-sheet junction or a loop-?-helix junction. A method of controlling protein activity and hosts including proteins with controlled activity are also provided. Also, intein modified proteins and plants containing intein modified proteins are provided.Type: ApplicationFiled: November 6, 2009Publication date: May 12, 2011Applicant: Agrivida, Inc.Inventors: Binzhang Shen, Gabor Lazar, Humberto de la Vega, James Apgar, Philip Lessard, R. Michael Raab
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Publication number: 20100113439Abstract: Novel derivatives of enfumafungin are disclosed herein, along with their pharmaceutically acceptable salts, hydrates and prodrugs. Also disclosed are compositions comprising such compounds, methods of preparing such compounds and methods of using such compounds as antifungal agents and/or inhibitors of (1,3)-?-D-glucan synthase. The disclosed compounds, their pharmaceutically acceptable salts, hydrates and prodrugs, as well as compositions comprising such compounds, salts, hydrates and prodrugs, are useful for treating and/or preventing fungal infections and associated diseases and conditions.Type: ApplicationFiled: August 7, 2009Publication date: May 6, 2010Applicants: Merck & Co., Inc., Scynexis, Inc.Inventors: Mark L. Greenlee, Robert Wilkening, James Apgar, Donald Sperbeck, Kenneth J. Wildonger, Dongfang Meng, Dann L. Parker, JR., Gregory J. Pacofsky, Brian H. Heasley, Ahmed Mamai, Kingsley Nelson